lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells
Abstract Background Anoikis resistance plays a critical role in the tumor metastasis by allowing survival of cancer cells in the systemic circulation. We previously showed that long non-coding RNAs APOC1P1-3 (lncRNA APOC1P1-3) inhibit apoptosis of breast cancer cells. In this study, we explored its...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-021-01916-w |
| _version_ | 1818937898652663808 |
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| author | Qi Lu Li Wang Yabiao Gao Ping Zhu Luying Li Xue Wang Youping Jin Xiuling Zhi Jerry Yu Xin Li Xingjun Qin Ping Zhou |
| author_facet | Qi Lu Li Wang Yabiao Gao Ping Zhu Luying Li Xue Wang Youping Jin Xiuling Zhi Jerry Yu Xin Li Xingjun Qin Ping Zhou |
| author_sort | Qi Lu |
| collection | DOAJ |
| description | Abstract Background Anoikis resistance plays a critical role in the tumor metastasis by allowing survival of cancer cells in the systemic circulation. We previously showed that long non-coding RNAs APOC1P1-3 (lncRNA APOC1P1-3) inhibit apoptosis of breast cancer cells. In this study, we explored its role in anoikis resistance. Methods We induced anoikis resistance in two breast cancer cell lines (MCF-7 and MDA-MB-231) under anchorage-independent culture conditions and studied lncRNA APOC1P1-3 effects on apoptosis. Using Dual-Luciferase activity assay, we determined whether it specifically binds to miRNA-188-3P. We further explored its role in lung metastasis by injecting MDA-MB-231 and MDA-MB-231-APOC1P1-3-knock-down cells in female BALB/c nude mice. Results We found that lncRNA APOC1P1-3 suppressed early apoptosis of these cells (demonstrated by gain or loss of their function, respectively) and promoted anoikis resistance via reducing activated- Caspase 3, 8, 9 and PARP. Moreover, it specifically binds to the target miRNA-188-3p acting as a “sponge” to block the inhibition of Bcl-2 (an anti-apoptosis protein). Conclusions Our study supports a theory that lncRNA APOC1P1-3 can promote development of breast cancer metastasis via anoikis resistance by specifically binding to miRNA-188-3p to block the inhibition of Bcl-2. |
| first_indexed | 2024-12-20T05:59:17Z |
| format | Article |
| id | doaj.art-f4fb366a9827498c9280a30aaeb2293a |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-20T05:59:17Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-f4fb366a9827498c9280a30aaeb2293a2022-12-21T19:50:56ZengBMCCancer Cell International1475-28672021-04-0121111310.1186/s12935-021-01916-wlncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cellsQi Lu0Li Wang1Yabiao Gao2Ping Zhu3Luying Li4Xue Wang5Youping Jin6Xiuling Zhi7Jerry Yu8Xin Li9Xingjun Qin10Ping Zhou11Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityInstitutes of Biomedical Sciences, Fudan UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityDepartment of Medicine, University of LouisvilleDepartment of Oral & Maxillofacial – Head & Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral & Maxillofacial – Head & Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan UniversityAbstract Background Anoikis resistance plays a critical role in the tumor metastasis by allowing survival of cancer cells in the systemic circulation. We previously showed that long non-coding RNAs APOC1P1-3 (lncRNA APOC1P1-3) inhibit apoptosis of breast cancer cells. In this study, we explored its role in anoikis resistance. Methods We induced anoikis resistance in two breast cancer cell lines (MCF-7 and MDA-MB-231) under anchorage-independent culture conditions and studied lncRNA APOC1P1-3 effects on apoptosis. Using Dual-Luciferase activity assay, we determined whether it specifically binds to miRNA-188-3P. We further explored its role in lung metastasis by injecting MDA-MB-231 and MDA-MB-231-APOC1P1-3-knock-down cells in female BALB/c nude mice. Results We found that lncRNA APOC1P1-3 suppressed early apoptosis of these cells (demonstrated by gain or loss of their function, respectively) and promoted anoikis resistance via reducing activated- Caspase 3, 8, 9 and PARP. Moreover, it specifically binds to the target miRNA-188-3p acting as a “sponge” to block the inhibition of Bcl-2 (an anti-apoptosis protein). Conclusions Our study supports a theory that lncRNA APOC1P1-3 can promote development of breast cancer metastasis via anoikis resistance by specifically binding to miRNA-188-3p to block the inhibition of Bcl-2.https://doi.org/10.1186/s12935-021-01916-wBreast cancerLncRNA APOC1P1-3Anoikis resistanceMiRNA-188-3pTumor metastasis |
| spellingShingle | Qi Lu Li Wang Yabiao Gao Ping Zhu Luying Li Xue Wang Youping Jin Xiuling Zhi Jerry Yu Xin Li Xingjun Qin Ping Zhou lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells Cancer Cell International Breast cancer LncRNA APOC1P1-3 Anoikis resistance MiRNA-188-3p Tumor metastasis |
| title | lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells |
| title_full | lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells |
| title_fullStr | lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells |
| title_full_unstemmed | lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells |
| title_short | lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells |
| title_sort | lncrna apoc1p1 3 promoting anoikis resistance of breast cancer cells |
| topic | Breast cancer LncRNA APOC1P1-3 Anoikis resistance MiRNA-188-3p Tumor metastasis |
| url | https://doi.org/10.1186/s12935-021-01916-w |
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