Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological Pathways
Introduction: Corynebacteria represent often-neglected etiological agents of post-traumatic and/or post-operative bone and joint infection (BJI). We describe here clinical characteristics and bacteriological determinants of this condition.Methods: A retrospective cohort study described characteristi...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2020.539501/full |
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| author | Pierre Chauvelot Pierre Chauvelot Pierre Chauvelot Tristan Ferry Tristan Ferry Tristan Ferry Virginie Tafani Alan Diot Jason Tasse Jason Tasse Anne Conrad Anne Conrad Anne Conrad Christian Chidiac Christian Chidiac Christian Chidiac Evelyne Braun Evelyne Braun Sébastien Lustig Sébastien Lustig Frédéric Laurent Frédéric Laurent Frédéric Laurent Florent Valour Florent Valour Florent Valour |
| author_facet | Pierre Chauvelot Pierre Chauvelot Pierre Chauvelot Tristan Ferry Tristan Ferry Tristan Ferry Virginie Tafani Alan Diot Jason Tasse Jason Tasse Anne Conrad Anne Conrad Anne Conrad Christian Chidiac Christian Chidiac Christian Chidiac Evelyne Braun Evelyne Braun Sébastien Lustig Sébastien Lustig Frédéric Laurent Frédéric Laurent Frédéric Laurent Florent Valour Florent Valour Florent Valour |
| author_sort | Pierre Chauvelot |
| collection | DOAJ |
| description | Introduction: Corynebacteria represent often-neglected etiological agents of post-traumatic and/or post-operative bone and joint infection (BJI). We describe here clinical characteristics and bacteriological determinants of this condition.Methods: A retrospective cohort study described characteristics, outcome and determinants of treatment failure of all patients with proven Corynebacterium spp. BJI (i.e., ≥2 culture-positive gold-standard samples). Available strains were further characterized regarding their antibiotic susceptibilies, abilities to form early (BioFilm Ring Test®) and mature (crystal violet staining method) biofilms and to invade osteoblasts (gentamicin protection assay).Results: The 51 included BJI were mostly chronic (88.2%), orthopedic device-related (74.5%) and polymicrobial (78.4%). After a follow-up of 60.7 weeks (IQR, 30.1–115.1), 20 (39.2%) treatment failures were observed, including 4 Corynebacterium-documented relapses, mostly associated with non-optimal surgical management (OR 7.291; p = 0.039). Internalization rate within MG63 human osteoblasts was higher for strains isolated from delayed (>3 months) BJI (p < 0.001). Infection of murine osteoblasts deleted for the β1-integrin resulted in a drastic reduction in the internalization rate. No difference was observed regarding biofilm formation.Conclusions: Surgical management plays a crucial role in outcome of BJI involving corynebacteria, as often chronic and device-associated infections. Sanctuarisation within osteoblasts, implicating the β1 cellular integrin, may represent a pivotal virulence factor associated with BJI chronicity. |
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| id | doaj.art-f503091ebe6e49d0a97f8a177baa5a5c |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-17T03:29:53Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-f503091ebe6e49d0a97f8a177baa5a5c2022-12-21T22:05:18ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2021-01-01710.3389/fmed.2020.539501539501Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological PathwaysPierre Chauvelot0Pierre Chauvelot1Pierre Chauvelot2Tristan Ferry3Tristan Ferry4Tristan Ferry5Virginie Tafani6Alan Diot7Jason Tasse8Jason Tasse9Anne Conrad10Anne Conrad11Anne Conrad12Christian Chidiac13Christian Chidiac14Christian Chidiac15Evelyne Braun16Evelyne Braun17Sébastien Lustig18Sébastien Lustig19Frédéric Laurent20Frédéric Laurent21Frédéric Laurent22Florent Valour23Florent Valour24Florent Valour25Departement of Infectious Diseases, Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceDepartement of Infectious Diseases, Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceBioFilm Control, Saint-Beauzire, FranceDepartement of Infectious Diseases, Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceDepartement of Infectious Diseases, Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceDepartement of Infectious Diseases, Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceOrthopedic Surgery Unit, Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceLaboratory of bacteriology, French National Reference Centre for Staphylococci, Hospices Civils de Lyon, Lyon, FranceDepartement of Infectious Diseases, Hospices Civils de Lyon, Lyon, FranceFrench Regional Reference Center for Complex Bone and Joint Infection (CRIOAc), Hospices Civils de Lyon, Lyon, FranceInternational Centre for Research in Infectiology, INSERM U1111, Claude Bernard Lyon 1 University, Lyon, FranceIntroduction: Corynebacteria represent often-neglected etiological agents of post-traumatic and/or post-operative bone and joint infection (BJI). We describe here clinical characteristics and bacteriological determinants of this condition.Methods: A retrospective cohort study described characteristics, outcome and determinants of treatment failure of all patients with proven Corynebacterium spp. BJI (i.e., ≥2 culture-positive gold-standard samples). Available strains were further characterized regarding their antibiotic susceptibilies, abilities to form early (BioFilm Ring Test®) and mature (crystal violet staining method) biofilms and to invade osteoblasts (gentamicin protection assay).Results: The 51 included BJI were mostly chronic (88.2%), orthopedic device-related (74.5%) and polymicrobial (78.4%). After a follow-up of 60.7 weeks (IQR, 30.1–115.1), 20 (39.2%) treatment failures were observed, including 4 Corynebacterium-documented relapses, mostly associated with non-optimal surgical management (OR 7.291; p = 0.039). Internalization rate within MG63 human osteoblasts was higher for strains isolated from delayed (>3 months) BJI (p < 0.001). Infection of murine osteoblasts deleted for the β1-integrin resulted in a drastic reduction in the internalization rate. No difference was observed regarding biofilm formation.Conclusions: Surgical management plays a crucial role in outcome of BJI involving corynebacteria, as often chronic and device-associated infections. Sanctuarisation within osteoblasts, implicating the β1 cellular integrin, may represent a pivotal virulence factor associated with BJI chronicity.https://www.frontiersin.org/articles/10.3389/fmed.2020.539501/fullCorynebacteriumosteoblastsbiofilmbone and joint infectionintracellular |
| spellingShingle | Pierre Chauvelot Pierre Chauvelot Pierre Chauvelot Tristan Ferry Tristan Ferry Tristan Ferry Virginie Tafani Alan Diot Jason Tasse Jason Tasse Anne Conrad Anne Conrad Anne Conrad Christian Chidiac Christian Chidiac Christian Chidiac Evelyne Braun Evelyne Braun Sébastien Lustig Sébastien Lustig Frédéric Laurent Frédéric Laurent Frédéric Laurent Florent Valour Florent Valour Florent Valour Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological Pathways Frontiers in Medicine Corynebacterium osteoblasts biofilm bone and joint infection intracellular |
| title | Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological Pathways |
| title_full | Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological Pathways |
| title_fullStr | Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological Pathways |
| title_full_unstemmed | Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological Pathways |
| title_short | Bone and Joint Infection Involving Corynebacterium spp.: From Clinical Features to Pathophysiological Pathways |
| title_sort | bone and joint infection involving corynebacterium spp from clinical features to pathophysiological pathways |
| topic | Corynebacterium osteoblasts biofilm bone and joint infection intracellular |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2020.539501/full |
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