Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models.
BACKGROUND: A strategy to combat infectious diseases, including neglected tropical diseases (NTDs), will depend on the development of reliable epidemiological surveillance methods. To establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoa...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC4117437?pdf=render |
| _version_ | 1819092086115270656 |
|---|---|
| author | Yoshito Fujii Satoshi Kaneko Samson Muuo Nzou Matilu Mwau Sammy M Njenga Chihiro Tanigawa James Kimotho Anne Wanjiru Mwangi Ibrahim Kiche Sohkichi Matsumoto Mamiko Niki Mayuko Osada-Oka Yoshio Ichinose Manabu Inoue Makoto Itoh Hiroshi Tachibana Kazunari Ishii Takafumi Tsuboi Lay Myint Yoshida Dinesh Mondal Rashidul Haque Shinjiro Hamano Mwatasa Changoma Tomonori Hoshi Ken-Ichi Kamo Mohamed Karama Masashi Miura Kenji Hirayama |
| author_facet | Yoshito Fujii Satoshi Kaneko Samson Muuo Nzou Matilu Mwau Sammy M Njenga Chihiro Tanigawa James Kimotho Anne Wanjiru Mwangi Ibrahim Kiche Sohkichi Matsumoto Mamiko Niki Mayuko Osada-Oka Yoshio Ichinose Manabu Inoue Makoto Itoh Hiroshi Tachibana Kazunari Ishii Takafumi Tsuboi Lay Myint Yoshida Dinesh Mondal Rashidul Haque Shinjiro Hamano Mwatasa Changoma Tomonori Hoshi Ken-Ichi Kamo Mohamed Karama Masashi Miura Kenji Hirayama |
| author_sort | Yoshito Fujii |
| collection | DOAJ |
| description | BACKGROUND: A strategy to combat infectious diseases, including neglected tropical diseases (NTDs), will depend on the development of reliable epidemiological surveillance methods. To establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in Kenya. METHODS: We developed a microsphere-based immuno-assay system to simultaneously measure the individual levels of plasma antibody (IgG) against 8 antigens derived from 6 pathogens: Entamoeba histolytica (C-IgL), Leishmania donovani (KRP42), Toxoplasma gondii (SAG1), Wuchereria bancrofti (SXP1), HIV (gag, gp120 and gp41), and Vibrio cholerae (cholera toxin). The assay system was validated using appropriate control samples. The assay system was applied for 3411 blood samples collected from the general population randomly selected from two health and demographic surveillance system (HDSS) cohorts in the coastal and western regions of Kenya. The immunoassay values distribution for each antigen was mathematically defined by a finite mixture model, and cut-off values were optimized. FINDINGS: Sensitivities and specificities for each antigen ranged between 71 and 100%. Seroprevalences for each pathogen from the Kwale and Mbita HDSS sites (respectively) were as follows: HIV, 3.0% and 20.1%; L. donovani, 12.6% and 17.3%; E. histolytica, 12.8% and 16.6%; and T. gondii, 30.9% and 28.2%. Seroprevalences of W. bancrofti and V. cholerae showed relatively high figures, especially among children. The results might be affected by immunological cross reactions between W. bancrofti-SXP1 and other parasitic infections; and cholera toxin and the enterotoxigenic E. coli (ETEC), respectively. INTERPRETATION: A microsphere-based multi-serological assay system can provide an opportunity to comprehensively grasp epidemiological features for NTDs. By adding pathogens and antigens of interest, optimized made-to-order high-quality programs can be established to utilize limited resources to effectively control NTDs in Africa. |
| first_indexed | 2024-12-21T22:50:01Z |
| format | Article |
| id | doaj.art-f50be232602147ef8dff2664a15e6fe1 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-21T22:50:01Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-f50be232602147ef8dff2664a15e6fe12022-12-21T18:47:36ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352014-01-0187e304010.1371/journal.pntd.0003040Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models.Yoshito FujiiSatoshi KanekoSamson Muuo NzouMatilu MwauSammy M NjengaChihiro TanigawaJames KimothoAnne Wanjiru MwangiIbrahim KicheSohkichi MatsumotoMamiko NikiMayuko Osada-OkaYoshio IchinoseManabu InoueMakoto ItohHiroshi TachibanaKazunari IshiiTakafumi TsuboiLay Myint YoshidaDinesh MondalRashidul HaqueShinjiro HamanoMwatasa ChangomaTomonori HoshiKen-Ichi KamoMohamed KaramaMasashi MiuraKenji HirayamaBACKGROUND: A strategy to combat infectious diseases, including neglected tropical diseases (NTDs), will depend on the development of reliable epidemiological surveillance methods. To establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in Kenya. METHODS: We developed a microsphere-based immuno-assay system to simultaneously measure the individual levels of plasma antibody (IgG) against 8 antigens derived from 6 pathogens: Entamoeba histolytica (C-IgL), Leishmania donovani (KRP42), Toxoplasma gondii (SAG1), Wuchereria bancrofti (SXP1), HIV (gag, gp120 and gp41), and Vibrio cholerae (cholera toxin). The assay system was validated using appropriate control samples. The assay system was applied for 3411 blood samples collected from the general population randomly selected from two health and demographic surveillance system (HDSS) cohorts in the coastal and western regions of Kenya. The immunoassay values distribution for each antigen was mathematically defined by a finite mixture model, and cut-off values were optimized. FINDINGS: Sensitivities and specificities for each antigen ranged between 71 and 100%. Seroprevalences for each pathogen from the Kwale and Mbita HDSS sites (respectively) were as follows: HIV, 3.0% and 20.1%; L. donovani, 12.6% and 17.3%; E. histolytica, 12.8% and 16.6%; and T. gondii, 30.9% and 28.2%. Seroprevalences of W. bancrofti and V. cholerae showed relatively high figures, especially among children. The results might be affected by immunological cross reactions between W. bancrofti-SXP1 and other parasitic infections; and cholera toxin and the enterotoxigenic E. coli (ETEC), respectively. INTERPRETATION: A microsphere-based multi-serological assay system can provide an opportunity to comprehensively grasp epidemiological features for NTDs. By adding pathogens and antigens of interest, optimized made-to-order high-quality programs can be established to utilize limited resources to effectively control NTDs in Africa.http://europepmc.org/articles/PMC4117437?pdf=render |
| spellingShingle | Yoshito Fujii Satoshi Kaneko Samson Muuo Nzou Matilu Mwau Sammy M Njenga Chihiro Tanigawa James Kimotho Anne Wanjiru Mwangi Ibrahim Kiche Sohkichi Matsumoto Mamiko Niki Mayuko Osada-Oka Yoshio Ichinose Manabu Inoue Makoto Itoh Hiroshi Tachibana Kazunari Ishii Takafumi Tsuboi Lay Myint Yoshida Dinesh Mondal Rashidul Haque Shinjiro Hamano Mwatasa Changoma Tomonori Hoshi Ken-Ichi Kamo Mohamed Karama Masashi Miura Kenji Hirayama Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. PLoS Neglected Tropical Diseases |
| title | Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. |
| title_full | Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. |
| title_fullStr | Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. |
| title_full_unstemmed | Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. |
| title_short | Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. |
| title_sort | serological surveillance development for tropical infectious diseases using simultaneous microsphere based multiplex assays and finite mixture models |
| url | http://europepmc.org/articles/PMC4117437?pdf=render |
| work_keys_str_mv | AT yoshitofujii serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT satoshikaneko serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT samsonmuuonzou serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT matilumwau serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT sammymnjenga serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT chihirotanigawa serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT jameskimotho serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT annewanjirumwangi serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT ibrahimkiche serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT sohkichimatsumoto serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT mamikoniki serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT mayukoosadaoka serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT yoshioichinose serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT manabuinoue serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT makotoitoh serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT hiroshitachibana serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT kazunariishii serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT takafumitsuboi serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT laymyintyoshida serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT dineshmondal serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT rashidulhaque serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT shinjirohamano serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT mwatasachangoma serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT tomonorihoshi serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT kenichikamo serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT mohamedkarama serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT masashimiura serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels AT kenjihirayama serologicalsurveillancedevelopmentfortropicalinfectiousdiseasesusingsimultaneousmicrospherebasedmultiplexassaysandfinitemixturemodels |