Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/8868 |
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| author | Mako Kobayashi Naoki Ishida Yoshihide Hashimoto Jun Negishi Hideki Saga Yoshihiro Sasaki Kazunari Akiyoshi Tsuyoshi Kimura Akio Kishida |
| author_facet | Mako Kobayashi Naoki Ishida Yoshihide Hashimoto Jun Negishi Hideki Saga Yoshihiro Sasaki Kazunari Akiyoshi Tsuyoshi Kimura Akio Kishida |
| author_sort | Mako Kobayashi |
| collection | DOAJ |
| description | Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling. |
| first_indexed | 2024-03-09T09:56:51Z |
| format | Article |
| id | doaj.art-f50ddaa59c2443f6a173e39fa3da8365 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:56:51Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f50ddaa59c2443f6a173e39fa3da83652023-12-01T23:46:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316886810.3390/ijms23168868Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized TissuesMako Kobayashi0Naoki Ishida1Yoshihide Hashimoto2Jun Negishi3Hideki Saga4Yoshihiro Sasaki5Kazunari Akiyoshi6Tsuyoshi Kimura7Akio Kishida8Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, JapanInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, JapanInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, JapanDepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, JapanKM Biologics Co., Ltd., 1314-1 Kyokushi Kawabe, Kikuchi-shi 869-1298, JapanDepartment of Polymer Chemistry, Graduate School of Engineering, A3-317, Kyoto University, Katsura, Nishikyo-ku 615-8510, JapanDepartment of Polymer Chemistry, Graduate School of Engineering, A3-317, Kyoto University, Katsura, Nishikyo-ku 615-8510, JapanInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, JapanInstitute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku 101-0062, JapanDecellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling.https://www.mdpi.com/1422-0067/23/16/8868decellularized tissuematrix-bound nanovesicleshigh-hydrostatic pressure |
| spellingShingle | Mako Kobayashi Naoki Ishida Yoshihide Hashimoto Jun Negishi Hideki Saga Yoshihiro Sasaki Kazunari Akiyoshi Tsuyoshi Kimura Akio Kishida Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues International Journal of Molecular Sciences decellularized tissue matrix-bound nanovesicles high-hydrostatic pressure |
| title | Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues |
| title_full | Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues |
| title_fullStr | Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues |
| title_full_unstemmed | Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues |
| title_short | Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues |
| title_sort | extraction and biological evaluation of matrix bound nanovesicles mbvs from high hydrostatic pressure decellularized tissues |
| topic | decellularized tissue matrix-bound nanovesicles high-hydrostatic pressure |
| url | https://www.mdpi.com/1422-0067/23/16/8868 |
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