MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer
Abstract Background Ovarian cancer is the leading lethal, gynecological malignancy in the United States. No doubt, the continued morbidity and mortality of ovarian cancer reflects a poor understanding of invasive mechanisms. Recent studies reveal that ovarian cancers express aberrant microRNAs (miRN...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-017-0328-1 |
| _version_ | 1797969724575842304 |
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| author | Jun Wei Lahong Zhang Jennifer Li Shuguang Zhu Minghui Tai Clifford W. Mason Julia A. Chapman Evelyn A. Reynolds Carl P Weiner Helen H Zhou |
| author_facet | Jun Wei Lahong Zhang Jennifer Li Shuguang Zhu Minghui Tai Clifford W. Mason Julia A. Chapman Evelyn A. Reynolds Carl P Weiner Helen H Zhou |
| author_sort | Jun Wei |
| collection | DOAJ |
| description | Abstract Background Ovarian cancer is the leading lethal, gynecological malignancy in the United States. No doubt, the continued morbidity and mortality of ovarian cancer reflects a poor understanding of invasive mechanisms. Recent studies reveal that ovarian cancers express aberrant microRNAs (miRNAs or miRs), some of which have oncogenic or tumor suppressor properties. Several studies suggested that miR-205 is involved in tumorigenesis. Presently, we investigate the molecular mechanisms and target of miR-205 in ovarian cancer. Methods Quantitative real-time polymerase chain reaction and western blot were performed to assess miR-205 and transcription factor 21 (TCF21) expression in ovarian cancer and normal ovary samples. The effect of miR-205 on TCF21 was determined by luciferase reporter assay and western blot. The effect of miR-205 and TCF21 on cell invasion was quantitated using transwell invasion assay. Result miR-205 expression was increased in ovarian cancer and it promoted the invasive behavior of ovarian cancer cell lines (OVCAR-5, OVCAR-8 and SKOV-3). miR-205 directly targeted TCF21, which was significantly decreased in ovarian cancer tissue. miR-205 inhibited TCF21 expression and as a consequence blunted the inhibitory effect of TCF21 on cell invasion. Matrix Metalloproteinases (MMPs) play an important role in cancer invasion and metastasis. TCF21 inhibited MMP-2 and MMP-10 and decreased ovarian cancer cell invasion. Co-transfection of TCF21 expression plasmid with miR-205 mimic diminished the inhibitory effect of TCF21 on MMP-2 and MMP-10 in ovarian cancer cells. Conclusion miR-205 appears to have an important role in the spread of ovarian cancer by targeting TCF21. These findings offer a new mechanism of ovarian cancer tumorigenesis, which could be useful for the development of new therapeutic approaches to ovarian cancer treatment. |
| first_indexed | 2024-04-11T03:06:45Z |
| format | Article |
| id | doaj.art-f514a38c42c442668adbd87966957372 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:06:45Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-f514a38c42c442668adbd879669573722023-01-02T13:12:54ZengBMCJournal of Ovarian Research1757-22152017-05-0110111110.1186/s13048-017-0328-1MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancerJun Wei0Lahong Zhang1Jennifer Li2Shuguang Zhu3Minghui Tai4Clifford W. Mason5Julia A. Chapman6Evelyn A. Reynolds7Carl P Weiner8Helen H Zhou9Department of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Clinical Laboratory, The Affiliated Hospital of Hangzhou Normal UniversityDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterDepartment of Obstetrics and Gynecology, University of Kansas Medical CenterAbstract Background Ovarian cancer is the leading lethal, gynecological malignancy in the United States. No doubt, the continued morbidity and mortality of ovarian cancer reflects a poor understanding of invasive mechanisms. Recent studies reveal that ovarian cancers express aberrant microRNAs (miRNAs or miRs), some of which have oncogenic or tumor suppressor properties. Several studies suggested that miR-205 is involved in tumorigenesis. Presently, we investigate the molecular mechanisms and target of miR-205 in ovarian cancer. Methods Quantitative real-time polymerase chain reaction and western blot were performed to assess miR-205 and transcription factor 21 (TCF21) expression in ovarian cancer and normal ovary samples. The effect of miR-205 on TCF21 was determined by luciferase reporter assay and western blot. The effect of miR-205 and TCF21 on cell invasion was quantitated using transwell invasion assay. Result miR-205 expression was increased in ovarian cancer and it promoted the invasive behavior of ovarian cancer cell lines (OVCAR-5, OVCAR-8 and SKOV-3). miR-205 directly targeted TCF21, which was significantly decreased in ovarian cancer tissue. miR-205 inhibited TCF21 expression and as a consequence blunted the inhibitory effect of TCF21 on cell invasion. Matrix Metalloproteinases (MMPs) play an important role in cancer invasion and metastasis. TCF21 inhibited MMP-2 and MMP-10 and decreased ovarian cancer cell invasion. Co-transfection of TCF21 expression plasmid with miR-205 mimic diminished the inhibitory effect of TCF21 on MMP-2 and MMP-10 in ovarian cancer cells. Conclusion miR-205 appears to have an important role in the spread of ovarian cancer by targeting TCF21. These findings offer a new mechanism of ovarian cancer tumorigenesis, which could be useful for the development of new therapeutic approaches to ovarian cancer treatment.http://link.springer.com/article/10.1186/s13048-017-0328-1microRNA(miR)-205TCF21Ovarian Cancer |
| spellingShingle | Jun Wei Lahong Zhang Jennifer Li Shuguang Zhu Minghui Tai Clifford W. Mason Julia A. Chapman Evelyn A. Reynolds Carl P Weiner Helen H Zhou MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer Journal of Ovarian Research microRNA(miR)-205 TCF21 Ovarian Cancer |
| title | MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer |
| title_full | MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer |
| title_fullStr | MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer |
| title_full_unstemmed | MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer |
| title_short | MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer |
| title_sort | microrna 205 promotes cell invasion by repressing tcf21 in human ovarian cancer |
| topic | microRNA(miR)-205 TCF21 Ovarian Cancer |
| url | http://link.springer.com/article/10.1186/s13048-017-0328-1 |
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