The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro

Red spinach (Amaranthus tricolor L.) is a commercially valuable vegetable known for its high nutritional content and immune-boosting properties, making it popular among the Indonesian population. However, red spinach cultivation in Indonesia is still relatively low and inconsistent, necessitating ef...

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Main Authors: Kishy Dhea Herlanda, Endang Nurcahyani, Mahfut Mahfut, Tundjung Tripeni Handayani
Format: Article
Language:Indonesian
Published: Muhammadiyah University Press 2023-11-01
Series:Bioeksperimen: Jurnal Penelitian Biologi
Subjects:
Online Access:https://journals.ums.ac.id/index.php/bioeksperimen/article/view/22948
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author Kishy Dhea Herlanda
Endang Nurcahyani
Mahfut Mahfut
Tundjung Tripeni Handayani
author_facet Kishy Dhea Herlanda
Endang Nurcahyani
Mahfut Mahfut
Tundjung Tripeni Handayani
author_sort Kishy Dhea Herlanda
collection DOAJ
description Red spinach (Amaranthus tricolor L.) is a commercially valuable vegetable known for its high nutritional content and immune-boosting properties, making it popular among the Indonesian population. However, red spinach cultivation in Indonesia is still relatively low and inconsistent, necessitating efforts to enhance its cultivation. One potential approach is through in vitro culture techniques. This study aimed to investigate the impact of applying mung bean sprout [Vigna radiata (L.) R. Wilczek] extract on Murashige and Skoog medium, focusing on identifying the most effective concentration for in vitro growth of red spinach explants. The experimental design employed a Completely Randomized Design (CRD) with five concentration levels: 0%, 5%, 10%, 15%, and 20%. Each concentration was replicated five times, with each replication consisting of five red spinach seeds in individual culture bottles. Data analysis involved Levene's test, one-way ANOVA, and Tukey's Honestly Significant Difference (HSD) test at a significance level of 5%. The findings revealed that the application of mung bean sprout extract significantly influenced the height of plantlets, leaf count, root length, fresh weight, and soluble carbohydrate content of red spinach. The most effective concentration for promoting red spinach explant growth was determined to be 5%.
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spelling doaj.art-f51552c9e25540a7a7b367934ff594e02024-03-27T02:00:25ZindMuhammadiyah University PressBioeksperimen: Jurnal Penelitian Biologi2460-13652527-27992023-11-019211612610.23917/bioeksperimen.v9i2.229488228The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In VitroKishy Dhea Herlanda0Endang Nurcahyani1Mahfut Mahfut2Tundjung Tripeni Handayani3Biology Department, Faculty of Science and Mathematic, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1, Gedong Meneng, Rajabasa, Bandar Lampung, 35145Biology Department, Faculty of Science and Mathematic, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1, Gedong Meneng, Rajabasa, Bandar Lampung, 35145Biology Department, Faculty of Science and Mathematic, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1, Gedong Meneng, Rajabasa, Bandar Lampung, 35145Biology Department, Faculty of Science and Mathematic, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1, Gedong Meneng, Rajabasa, Bandar Lampung, 35145Red spinach (Amaranthus tricolor L.) is a commercially valuable vegetable known for its high nutritional content and immune-boosting properties, making it popular among the Indonesian population. However, red spinach cultivation in Indonesia is still relatively low and inconsistent, necessitating efforts to enhance its cultivation. One potential approach is through in vitro culture techniques. This study aimed to investigate the impact of applying mung bean sprout [Vigna radiata (L.) R. Wilczek] extract on Murashige and Skoog medium, focusing on identifying the most effective concentration for in vitro growth of red spinach explants. The experimental design employed a Completely Randomized Design (CRD) with five concentration levels: 0%, 5%, 10%, 15%, and 20%. Each concentration was replicated five times, with each replication consisting of five red spinach seeds in individual culture bottles. Data analysis involved Levene's test, one-way ANOVA, and Tukey's Honestly Significant Difference (HSD) test at a significance level of 5%. The findings revealed that the application of mung bean sprout extract significantly influenced the height of plantlets, leaf count, root length, fresh weight, and soluble carbohydrate content of red spinach. The most effective concentration for promoting red spinach explant growth was determined to be 5%.https://journals.ums.ac.id/index.php/bioeksperimen/article/view/22948cultivation, growth, in vitro, mung bean sprout extract, red spinach
spellingShingle Kishy Dhea Herlanda
Endang Nurcahyani
Mahfut Mahfut
Tundjung Tripeni Handayani
The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro
Bioeksperimen: Jurnal Penelitian Biologi
cultivation, growth, in vitro, mung bean sprout extract, red spinach
title The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro
title_full The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro
title_fullStr The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro
title_full_unstemmed The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro
title_short The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro
title_sort growth of red spinach amaranthus tricolor l after application of bean sprouts extract vigna radiata l r wilczek on murashige and skoog medium in vitro
topic cultivation, growth, in vitro, mung bean sprout extract, red spinach
url https://journals.ums.ac.id/index.php/bioeksperimen/article/view/22948
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