Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New
Members of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct management. Here, we present an overview of current st...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/7/1518 |
| _version_ | 1797526559702122496 |
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| author | Nina Lackner Wilfried Posch Cornelia Lass-Flörl |
| author_facet | Nina Lackner Wilfried Posch Cornelia Lass-Flörl |
| author_sort | Nina Lackner |
| collection | DOAJ |
| description | Members of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct management. Here, we present an overview of current state-of-the-art mucormycosis diagnoses, with a focus on recent developments in the molecular field. Classical diagnostic methods comprise histology/microscopy as well as culture and are still the gold standard. Newer molecular methods are evolving quickly and display great potential in early diagnosis, although standardization is still missing. Among them, quantitative PCR assays with or without melt curve analysis are most widely used to detect fungal DNA in clinical samples. Depending on the respective assay, sequencing of the resulting PCR product can be necessary for genus or even species identification. Further, DNA-based methods include microarrays and PCR-ESI-MS. However, general laboratory standards are still in development, meaning that molecular methods are currently limited to add-on analytics to culture and microscopy. |
| first_indexed | 2024-03-10T09:32:00Z |
| format | Article |
| id | doaj.art-f5165d16b7ec41b09195675bd9ce9045 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T09:32:00Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f5165d16b7ec41b09195675bd9ce90452023-11-22T04:26:58ZengMDPI AGMicroorganisms2076-26072021-07-0197151810.3390/microorganisms9071518Microbiological and Molecular Diagnosis of Mucormycosis: From Old to NewNina Lackner0Wilfried Posch1Cornelia Lass-Flörl2Department of Hygiene and Medical Microbiology, Medical University Innsbruck, 6020 Innsbruck, AustriaDepartment of Hygiene and Medical Microbiology, Medical University Innsbruck, 6020 Innsbruck, AustriaDepartment of Hygiene and Medical Microbiology, Medical University Innsbruck, 6020 Innsbruck, AustriaMembers of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct management. Here, we present an overview of current state-of-the-art mucormycosis diagnoses, with a focus on recent developments in the molecular field. Classical diagnostic methods comprise histology/microscopy as well as culture and are still the gold standard. Newer molecular methods are evolving quickly and display great potential in early diagnosis, although standardization is still missing. Among them, quantitative PCR assays with or without melt curve analysis are most widely used to detect fungal DNA in clinical samples. Depending on the respective assay, sequencing of the resulting PCR product can be necessary for genus or even species identification. Further, DNA-based methods include microarrays and PCR-ESI-MS. However, general laboratory standards are still in development, meaning that molecular methods are currently limited to add-on analytics to culture and microscopy.https://www.mdpi.com/2076-2607/9/7/1518MucoralesdiagnosisDNAPCRspecificitysensitivity |
| spellingShingle | Nina Lackner Wilfried Posch Cornelia Lass-Flörl Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New Microorganisms Mucorales diagnosis DNA PCR specificity sensitivity |
| title | Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New |
| title_full | Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New |
| title_fullStr | Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New |
| title_full_unstemmed | Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New |
| title_short | Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New |
| title_sort | microbiological and molecular diagnosis of mucormycosis from old to new |
| topic | Mucorales diagnosis DNA PCR specificity sensitivity |
| url | https://www.mdpi.com/2076-2607/9/7/1518 |
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