Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it>
<p>Abstract</p> <p>Background</p> <p>Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects <it>in planta</it> remain poorly understood. H...
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| Format: | Article |
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BMC
2012-11-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://www.biomedcentral.com/1471-2229/12/198 |
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| author | Munger Aurélie Coenen Karine Cantin Line Goulet Charles Vaillancourt Louis-Philippe Goulet Marie-Claire Tweddell Russell Sainsbury Frank Michaud Dominique |
| author_facet | Munger Aurélie Coenen Karine Cantin Line Goulet Charles Vaillancourt Louis-Philippe Goulet Marie-Claire Tweddell Russell Sainsbury Frank Michaud Dominique |
| author_sort | Munger Aurélie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects <it>in planta</it> remain poorly understood. Here we assessed the effects of corn cystatin II (CCII), a potent inhibitor of C1A cysteine (Cys) proteases considered for insect and pathogen control, on the leaf proteome and pathogen resistance status of potato lines constitutively expressing this protein.</p> <p>Results</p> <p>The leaf proteome of lines accumulating CCII at different levels was resolved by 2-dimensional gel electrophoresis and compared with the leaf proteome of a control (parental) line. Out of <it>ca.</it> 700 proteins monitored on 2-D gels, 23 were significantly up- or downregulated in CCII-expressing leaves, including 14 proteins detected <it>de novo</it> or up-regulated by more than five-fold compared to the control. Most up-regulated proteins were abiotic or biotic stress-responsive proteins, including different secretory peroxidases, wound inducible protease inhibitors and pathogenesis-related proteins. Accordingly, infection of leaf tissues by the fungal necrotroph <it>Botryris cinerea</it> was prevented in CCII-expressing plants, despite a null impact of CCII on growth of this pathogen and the absence of extracellular Cys protease targets for the inhibitor.</p> <p>Conclusions</p> <p>These data point to the onset of pleiotropic effects altering the leaf proteome in transgenic plants expressing recombinant protease inhibitors. They also show the potential of these proteins as ectopic modulators of stress responses <it>in planta</it>, useful to engineer biotic or abiotic stress tolerance in crop plants of economic significance.</p> |
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| language | English |
| last_indexed | 2024-12-10T18:30:59Z |
| publishDate | 2012-11-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-f51849e6b8514f09856d5b9cc310b31e2022-12-22T01:37:56ZengBMCBMC Plant Biology1471-22292012-11-0112119810.1186/1471-2229-12-198Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it>Munger AurélieCoenen KarineCantin LineGoulet CharlesVaillancourt Louis-PhilippeGoulet Marie-ClaireTweddell RussellSainsbury FrankMichaud Dominique<p>Abstract</p> <p>Background</p> <p>Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects <it>in planta</it> remain poorly understood. Here we assessed the effects of corn cystatin II (CCII), a potent inhibitor of C1A cysteine (Cys) proteases considered for insect and pathogen control, on the leaf proteome and pathogen resistance status of potato lines constitutively expressing this protein.</p> <p>Results</p> <p>The leaf proteome of lines accumulating CCII at different levels was resolved by 2-dimensional gel electrophoresis and compared with the leaf proteome of a control (parental) line. Out of <it>ca.</it> 700 proteins monitored on 2-D gels, 23 were significantly up- or downregulated in CCII-expressing leaves, including 14 proteins detected <it>de novo</it> or up-regulated by more than five-fold compared to the control. Most up-regulated proteins were abiotic or biotic stress-responsive proteins, including different secretory peroxidases, wound inducible protease inhibitors and pathogenesis-related proteins. Accordingly, infection of leaf tissues by the fungal necrotroph <it>Botryris cinerea</it> was prevented in CCII-expressing plants, despite a null impact of CCII on growth of this pathogen and the absence of extracellular Cys protease targets for the inhibitor.</p> <p>Conclusions</p> <p>These data point to the onset of pleiotropic effects altering the leaf proteome in transgenic plants expressing recombinant protease inhibitors. They also show the potential of these proteins as ectopic modulators of stress responses <it>in planta</it>, useful to engineer biotic or abiotic stress tolerance in crop plants of economic significance.</p>http://www.biomedcentral.com/1471-2229/12/198Transgenic cropsTransgene pleiotropyUnintended effectsStress/defense-related proteomeCorn cystatinPotato (<it>Solanum tuberosum</it>) |
| spellingShingle | Munger Aurélie Coenen Karine Cantin Line Goulet Charles Vaillancourt Louis-Philippe Goulet Marie-Claire Tweddell Russell Sainsbury Frank Michaud Dominique Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it> BMC Plant Biology Transgenic crops Transgene pleiotropy Unintended effects Stress/defense-related proteome Corn cystatin Potato (<it>Solanum tuberosum</it>) |
| title | Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it> |
| title_full | Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it> |
| title_fullStr | Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it> |
| title_full_unstemmed | Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it> |
| title_short | Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, <it>Solanum tuberosum</it> |
| title_sort | beneficial unintended effects of a cereal cystatin in transgenic lines of potato it solanum tuberosum it |
| topic | Transgenic crops Transgene pleiotropy Unintended effects Stress/defense-related proteome Corn cystatin Potato (<it>Solanum tuberosum</it>) |
| url | http://www.biomedcentral.com/1471-2229/12/198 |
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