Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.)
Lead (Pb) is one of the most important toxic heavy metals that reduce plant growth and development. Therefore, finding compounds that can alleviate the toxic effects of Pb is necessary. Nitric oxide (NO) is a signaling molecule that plays important roles in different physiological processes...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500139S.pdf |
| _version_ | 1818519850048290816 |
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| author | Sadeghipour Omid |
| author_facet | Sadeghipour Omid |
| author_sort | Sadeghipour Omid |
| collection | DOAJ |
| description | Lead (Pb) is one of the most important toxic heavy metals that reduce plant
growth and development. Therefore, finding compounds that can alleviate the
toxic effects of Pb is necessary. Nitric oxide (NO) is a signaling molecule
that plays important roles in different physiological processes in plants,
such as the response to biotic and abiotic stresses. A pot experiment was
conducted to study the effects of exogenously applied NO on Pb stress
tolerance in cowpea. Seeds were soaked at various concentrations of sodium
nitroprusside (SNP; NO donor) (0, 0.5 and 1 mM) for 20 h, and then sown in
pots containing 0 or 200 mg kg-1 Pb (NO3)2. The obtained results showed that
Pb stress significantly reduced the chlorophyll value, relative water
content (RWC) and net photosynthetic rate, but increased lipid peroxidation,
proline content and superoxide dismutase (SOD), catalase (CAT), ascorbate
peroxidase (APX) and glutathione reductase (GR) activities. Nonetheless,
different levels of NO significantly lowered Pb toxicity by further
increasing SOD, CAT, APX and GR activities as well as proline accumulation.
NO pretreatment also raised the chlorophyll value, RWC and net
photosynthetic rate but reduced lipid peroxidation. Both NO levels (0.5 and
1 mM) were effective in Pb stress tolerance; however, 0.5 mM was more
effective. These results indicate that NO pretreatment plays an important
role in protecting cowpea plants against Pb toxicity by enhancing
antioxidant enzyme activities and proline accumulation. |
| first_indexed | 2024-12-11T01:29:34Z |
| format | Article |
| id | doaj.art-f51a219357ef4ac2bc4d74d5c3a16ed4 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-11T01:29:34Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-f51a219357ef4ac2bc4d74d5c3a16ed42022-12-22T01:25:25ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168116517510.2298/ABS150325139S0354-46641500139SPretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.)Sadeghipour Omid0Islamic Azad University, Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Department of Agronomy, Tehran, IranLead (Pb) is one of the most important toxic heavy metals that reduce plant growth and development. Therefore, finding compounds that can alleviate the toxic effects of Pb is necessary. Nitric oxide (NO) is a signaling molecule that plays important roles in different physiological processes in plants, such as the response to biotic and abiotic stresses. A pot experiment was conducted to study the effects of exogenously applied NO on Pb stress tolerance in cowpea. Seeds were soaked at various concentrations of sodium nitroprusside (SNP; NO donor) (0, 0.5 and 1 mM) for 20 h, and then sown in pots containing 0 or 200 mg kg-1 Pb (NO3)2. The obtained results showed that Pb stress significantly reduced the chlorophyll value, relative water content (RWC) and net photosynthetic rate, but increased lipid peroxidation, proline content and superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) activities. Nonetheless, different levels of NO significantly lowered Pb toxicity by further increasing SOD, CAT, APX and GR activities as well as proline accumulation. NO pretreatment also raised the chlorophyll value, RWC and net photosynthetic rate but reduced lipid peroxidation. Both NO levels (0.5 and 1 mM) were effective in Pb stress tolerance; however, 0.5 mM was more effective. These results indicate that NO pretreatment plays an important role in protecting cowpea plants against Pb toxicity by enhancing antioxidant enzyme activities and proline accumulation.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500139S.pdfantioxidant enzymesnitric oxidePb stressphotosynthesisproline |
| spellingShingle | Sadeghipour Omid Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.) Archives of Biological Sciences antioxidant enzymes nitric oxide Pb stress photosynthesis proline |
| title | Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.) |
| title_full | Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.) |
| title_fullStr | Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.) |
| title_full_unstemmed | Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.) |
| title_short | Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.) |
| title_sort | pretreatment with nitric oxide reduces lead toxicity in cowpea vigna unguiculata l walp |
| topic | antioxidant enzymes nitric oxide Pb stress photosynthesis proline |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500139S.pdf |
| work_keys_str_mv | AT sadeghipouromid pretreatmentwithnitricoxidereducesleadtoxicityincowpeavignaunguiculatalwalp |