Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens
Studied was dissemination of avian influenza virus (AIV) in the organism of chickens after intranasal challenge with 10-100 LD50. The primary organ of accumulation of AIV A/H5N1 (A/Chicken/Kurgan/05/2005strain) is the respiratory tract (nasal mucosa), where the virus is registered in 18 hours after...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2012-04-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/799 |
| _version_ | 1797220977962123264 |
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| author | O. K. Demina Ar. A. Sergeev O. V. P'yankov A. N. Shikov Al. A. Sergeev S. A. Berillo E. I. Sergeeva A. P. Agafonov A. N. Sergeev |
| author_facet | O. K. Demina Ar. A. Sergeev O. V. P'yankov A. N. Shikov Al. A. Sergeev S. A. Berillo E. I. Sergeeva A. P. Agafonov A. N. Sergeev |
| author_sort | O. K. Demina |
| collection | DOAJ |
| description | Studied was dissemination of avian influenza virus (AIV) in the organism of chickens after intranasal challenge with 10-100 LD50. The primary organ of accumulation of AIV A/H5N1 (A/Chicken/Kurgan/05/2005strain) is the respiratory tract (nasal mucosa), where the virus is registered in 18 hours after challenge. The accumulation of pathogen is observed in many organs and serum of chicken in 30-32 hours after challenge. The animals die in 54 hours, the concentration of virus reaches critical value in all studied samples. The highest AIV loads (7 lg of chicken embryo infective dose - EID50/g or ml) are registered in lungs, blood serum and kidneys of chicken. The results of AIV loads measuring using titration and real time RT-PCR show high degree of correlation (r=0.89). |
| first_indexed | 2024-03-08T13:43:59Z |
| format | Article |
| id | doaj.art-f51a337a141842c3ad9a1910e8477c90 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:58:07Z |
| publishDate | 2012-04-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-f51a337a141842c3ad9a1910e8477c902024-04-05T16:47:08ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2012-04-0102(112)656810.21055/0370-1069-2012-2(112)-65-68783Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of ChickensO. K. Demina0Ar. A. Sergeev1O. V. P'yankov2A. N. Shikov3Al. A. Sergeev4S. A. Berillo5E. I. Sergeeva6A. P. Agafonov7A. N. Sergeev8State Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorState Research Centre of Virology and Biotechnology VectorStudied was dissemination of avian influenza virus (AIV) in the organism of chickens after intranasal challenge with 10-100 LD50. The primary organ of accumulation of AIV A/H5N1 (A/Chicken/Kurgan/05/2005strain) is the respiratory tract (nasal mucosa), where the virus is registered in 18 hours after challenge. The accumulation of pathogen is observed in many organs and serum of chicken in 30-32 hours after challenge. The animals die in 54 hours, the concentration of virus reaches critical value in all studied samples. The highest AIV loads (7 lg of chicken embryo infective dose - EID50/g or ml) are registered in lungs, blood serum and kidneys of chicken. The results of AIV loads measuring using titration and real time RT-PCR show high degree of correlation (r=0.89).https://journal.microbe.ru/jour/article/view/799avian influenza a/h5n1 viruschickenintranasal infectiondynamics of virus accumulationrt-pcr in real time |
| spellingShingle | O. K. Demina Ar. A. Sergeev O. V. P'yankov A. N. Shikov Al. A. Sergeev S. A. Berillo E. I. Sergeeva A. P. Agafonov A. N. Sergeev Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens Проблемы особо опасных инфекций avian influenza a/h5n1 virus chicken intranasal infection dynamics of virus accumulation rt-pcr in real time |
| title | Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens |
| title_full | Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens |
| title_fullStr | Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens |
| title_full_unstemmed | Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens |
| title_short | Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens |
| title_sort | dissemination of influenza a h5n1 virus after intranasal inoculation of chickens |
| topic | avian influenza a/h5n1 virus chicken intranasal infection dynamics of virus accumulation rt-pcr in real time |
| url | https://journal.microbe.ru/jour/article/view/799 |
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