The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathways
One of the serious consequences of brain stroke is depression, which affects a large number of patients. Finding new compounds that have antidepressant properties in these conditions can be very important. Therefore, in the present study, the antidepressant effects of apple pectin (AP) and citrus pe...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522300326X |
| _version_ | 1797804032524288000 |
|---|---|
| author | Zhibing Ai Yong Liu Xueyu Shi Wei Hu Yueliang Zhang Ran An Gongwun Lei |
| author_facet | Zhibing Ai Yong Liu Xueyu Shi Wei Hu Yueliang Zhang Ran An Gongwun Lei |
| author_sort | Zhibing Ai |
| collection | DOAJ |
| description | One of the serious consequences of brain stroke is depression, which affects a large number of patients. Finding new compounds that have antidepressant properties in these conditions can be very important. Therefore, in the present study, the antidepressant effects of apple pectin (AP) and citrus pectin (CP) in mice after induction of cerebral ischemia were studied. Seven days before transient middle cerebral artery occlusion (tMCAO) and three days thereafter, mice were given orally AP and CP by gavage (50 and 100 mg/kg). Forced swim test (FST), inclined beam-walking test and open field test were used to evaluate the behaviors of mice. Levels of IL-1β, INF-γ, TNF-α, IL-6 NF-κB-p65 and pSTAT3 were studied using enzyme-linked immunosorbent assay (ELISA) and the gene expressions of NF-κB-p65 and pSTAT3 were studied by qRT-PCR. Induction of cerebral I/R injury resulted in depressive-like behaviors in mice, which were confirmed by FST, inclined beam-walking test and OFT. However, beneficial effects of AP and CP were observed in reducing depressive-like behaviors in mice. The downregulation of proinflammatory cytokines and NF-κB-p65 and pSTAT3 proteins were observed as a result of oral AP and CP administration in cerebral I/R mice. AP and CP have antidepressant-like effects on cerebral I/R- induced depression due to reduced inflammation in the brain hippocampus. |
| first_indexed | 2024-03-13T05:30:02Z |
| format | Article |
| id | doaj.art-f5201cae9a94460481dae48ad8ccc409 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-13T05:30:02Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-f5201cae9a94460481dae48ad8ccc4092023-06-15T04:54:45ZengElsevierArabian Journal of Chemistry1878-53522023-08-01168104864The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathwaysZhibing Ai0Yong Liu1Xueyu Shi2Wei Hu3Yueliang Zhang4Ran An5Gongwun Lei6Institute of Neurobiogy, Xi'an Jiaotong University Basic Medical School, Xi’an 710061, China; Department of Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaInstitute of Neurobiogy, Xi'an Jiaotong University Basic Medical School, Xi’an 710061, China; Corresponding authors at: NO.76, Yanta West Road, Institute of Neurobiogy, Xi'an Jiaotong University Basic Medical School, Xi'an 710061, China.Department of Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaDepartment of Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaDepartment of Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaDepartment of Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaDepartment of Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, ChinaOne of the serious consequences of brain stroke is depression, which affects a large number of patients. Finding new compounds that have antidepressant properties in these conditions can be very important. Therefore, in the present study, the antidepressant effects of apple pectin (AP) and citrus pectin (CP) in mice after induction of cerebral ischemia were studied. Seven days before transient middle cerebral artery occlusion (tMCAO) and three days thereafter, mice were given orally AP and CP by gavage (50 and 100 mg/kg). Forced swim test (FST), inclined beam-walking test and open field test were used to evaluate the behaviors of mice. Levels of IL-1β, INF-γ, TNF-α, IL-6 NF-κB-p65 and pSTAT3 were studied using enzyme-linked immunosorbent assay (ELISA) and the gene expressions of NF-κB-p65 and pSTAT3 were studied by qRT-PCR. Induction of cerebral I/R injury resulted in depressive-like behaviors in mice, which were confirmed by FST, inclined beam-walking test and OFT. However, beneficial effects of AP and CP were observed in reducing depressive-like behaviors in mice. The downregulation of proinflammatory cytokines and NF-κB-p65 and pSTAT3 proteins were observed as a result of oral AP and CP administration in cerebral I/R mice. AP and CP have antidepressant-like effects on cerebral I/R- induced depression due to reduced inflammation in the brain hippocampus.http://www.sciencedirect.com/science/article/pii/S187853522300326XPost-Stroke DepressionInflammationCytokineMice |
| spellingShingle | Zhibing Ai Yong Liu Xueyu Shi Wei Hu Yueliang Zhang Ran An Gongwun Lei The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathways Arabian Journal of Chemistry Post-Stroke Depression Inflammation Cytokine Mice |
| title | The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathways |
| title_full | The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathways |
| title_fullStr | The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathways |
| title_full_unstemmed | The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathways |
| title_short | The protective effects of apple pectin and citrus pectins on post-cerebral I/R depression in mice: The role of NF-κB-p65 and pSTAT3 pathways |
| title_sort | protective effects of apple pectin and citrus pectins on post cerebral i r depression in mice the role of nf κb p65 and pstat3 pathways |
| topic | Post-Stroke Depression Inflammation Cytokine Mice |
| url | http://www.sciencedirect.com/science/article/pii/S187853522300326X |
| work_keys_str_mv | AT zhibingai theprotectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT yongliu theprotectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT xueyushi theprotectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT weihu theprotectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT yueliangzhang theprotectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT ranan theprotectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT gongwunlei theprotectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT zhibingai protectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT yongliu protectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT xueyushi protectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT weihu protectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT yueliangzhang protectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT ranan protectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways AT gongwunlei protectiveeffectsofapplepectinandcitruspectinsonpostcerebralirdepressioninmicetheroleofnfkbp65andpstat3pathways |