Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium
The benefits of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new Lactobacillus plantarum strains isolated from different dairy...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2017-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/262745 |
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| author | Mia Potočnjak Petra Pušić Jadranka Frece Maja Abram Tamara Janković Ivana Gobin |
| author_facet | Mia Potočnjak Petra Pušić Jadranka Frece Maja Abram Tamara Janković Ivana Gobin |
| author_sort | Mia Potočnjak |
| collection | DOAJ |
| description | The benefits of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new Lactobacillus plantarum strains isolated from different dairy products: cow′s cheese, sheep′s cheese and whey. In order to determine the antibacterial activity of yet unexplored L. plantarum strains against Salmonella enterica serotype Typhimurium, in vitro competition and co-culture tests were done. Furthermore, adhesion of these strains to Caco-2 cells and their influence on the adhesion of Salmonella were tested. Results showed the potential probiotic activity of isolated strains. L. plantarum strains survived in the presence of 1 % bile salts, they possessed acidification ability, antibacterial activity and significantly attenuated the growth of S. Typhimurium in brain heart infusion broth. All tested L. plantarum strains were able to adhere to Caco-2 cells and significantly impair the adhesion of S. Typhimurium. All three L. plantarum strains exhibited signifi cant probiotic potential and anti-Salmonella activity; therefore, further testing on in vivo models should follow. |
| first_indexed | 2024-04-24T09:30:31Z |
| format | Article |
| id | doaj.art-f523d3b1fc0f4cd180a19ad09ef72b37 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:30:31Z |
| publishDate | 2017-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-f523d3b1fc0f4cd180a19ad09ef72b372024-04-15T14:06:48ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062017-01-01551485410.17113/ftb.55.01.17.4693Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype TyphimuriumMia Potočnjak0Petra Pušić1Jadranka Frece2Maja Abram3Tamara Janković4Ivana Gobin5University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, CroatiaUniversity of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, CroatiaUniversity of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for General Microbiology and Food Microbiology, Pierottijeva 6, HR-10000 Zagreb, CroatiaUniversity of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, CroatiaUniversity of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, CroatiaUniversity of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, CroatiaThe benefits of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new Lactobacillus plantarum strains isolated from different dairy products: cow′s cheese, sheep′s cheese and whey. In order to determine the antibacterial activity of yet unexplored L. plantarum strains against Salmonella enterica serotype Typhimurium, in vitro competition and co-culture tests were done. Furthermore, adhesion of these strains to Caco-2 cells and their influence on the adhesion of Salmonella were tested. Results showed the potential probiotic activity of isolated strains. L. plantarum strains survived in the presence of 1 % bile salts, they possessed acidification ability, antibacterial activity and significantly attenuated the growth of S. Typhimurium in brain heart infusion broth. All tested L. plantarum strains were able to adhere to Caco-2 cells and significantly impair the adhesion of S. Typhimurium. All three L. plantarum strains exhibited signifi cant probiotic potential and anti-Salmonella activity; therefore, further testing on in vivo models should follow.https://hrcak.srce.hr/file/262745probiotic bacteriaantagonistic activitySalmonellamechanism of action |
| spellingShingle | Mia Potočnjak Petra Pušić Jadranka Frece Maja Abram Tamara Janković Ivana Gobin Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium Food Technology and Biotechnology probiotic bacteria antagonistic activity Salmonella mechanism of action |
| title | Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium |
| title_full | Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium |
| title_fullStr | Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium |
| title_full_unstemmed | Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium |
| title_short | Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium |
| title_sort | three new lactobacillus plantarum strains in the probiotic toolbox against gut pathogen salmonella enterica serotype typhimurium |
| topic | probiotic bacteria antagonistic activity Salmonella mechanism of action |
| url | https://hrcak.srce.hr/file/262745 |
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