Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary
Abstract Background Hunting constitutes an important industry in Europe. However, data on the prevalence of vector-borne bacteria in large game animal species are lacking from several countries. Blood or spleen samples (239 and 270, respectively) were taken from red, fallow and roe deer, as well as...
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1403-6 |
| _version_ | 1818315639864950784 |
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| author | Sándor Hornok László Sugár Isabel G. Fernández de Mera José de la Fuente Gábor Horváth Tibor Kovács Attila Micsutka Enikő Gönczi Barbara Flaisz Nóra Takács Róbert Farkas Marina L. Meli Regina Hofmann-Lehmann |
| author_facet | Sándor Hornok László Sugár Isabel G. Fernández de Mera José de la Fuente Gábor Horváth Tibor Kovács Attila Micsutka Enikő Gönczi Barbara Flaisz Nóra Takács Róbert Farkas Marina L. Meli Regina Hofmann-Lehmann |
| author_sort | Sándor Hornok |
| collection | DOAJ |
| description | Abstract Background Hunting constitutes an important industry in Europe. However, data on the prevalence of vector-borne bacteria in large game animal species are lacking from several countries. Blood or spleen samples (239 and 270, respectively) were taken from red, fallow and roe deer, as well as from water buffaloes, mouflons and wild boars in Hungary, followed by DNA extraction and molecular analyses for Anaplasma phagocytophilum, haemoplasmas and rickettsiae. Results Based on blood samples, the prevalence rate of A. phagocytophilum infection was significantly higher in red deer (97.9%) than in fallow deer (72.7%) and roe deer (60%), and in all these compared to mouflons (6.3%). In addition, 39.2% of the spleen samples from wild boars were PCR positive for A. phagocytophilum, but none of the buffalos. Based on blood samples, the prevalence rates of both Mycoplasma wenyonii (Mw) and ‘Candidatus M. haemobos’ (CMh) infections were significantly higher in buffaloes (Mw: 91.2%; CMh: 73.3%) than in red deer (Mw: 64.6%; CMh: 45.8%), and in both of them compared to fallow deer (Mw: 30.3%; CMh: 9.1%) and roe deer (Mw: 20%; CMh: 1.5%). The prevalence of Mw and CMh infection significantly correlated with the body sizes of these hosts. Furthermore, Mw was significantly more prevalent than CMh in buffaloes, red and roe deer. Mycoplasma ovis was detected in mouflons, M. suis in wild boars, R. helvetica in one fallow deer and one mouflon, and an unidentified Rickettsia sp. in a fallow deer. Conclusions Forest-dwelling game animal species were found to be important carriers of A. phagocytophilum. In contrast, animals grazing grassland (i.e. buffaloes) were less likely to get infected with this Ixodes ricinus-borne pathogen. Water buffaloes, deer species, mouflons and wild boars harbored haemoplasmas that may affect domestic ungulates. Evaluated animals with larger body size had significantly higher prevalence of infection with haemoplasmas compared to smaller deer species. The above host species rarely carried rickettsiae. |
| first_indexed | 2024-12-13T09:08:44Z |
| format | Article |
| id | doaj.art-f5255e2023ea46ffa853db00017f528f |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-13T09:08:44Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-f5255e2023ea46ffa853db00017f528f2022-12-21T23:53:00ZengBMCBMC Veterinary Research1746-61482018-03-011411710.1186/s12917-018-1403-6Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, HungarySándor Hornok0László Sugár1Isabel G. Fernández de Mera2José de la Fuente3Gábor Horváth4Tibor Kovács5Attila Micsutka6Enikő Gönczi7Barbara Flaisz8Nóra Takács9Róbert Farkas10Marina L. Meli11Regina Hofmann-Lehmann12Department of Parasitology and Zoology, University of Veterinary MedicineDepartment of Game Management and Ethology, Faculty of Agricultural and Environmental Sciences, University of KaposvárSaBio. Instituto de Investigación en Recursos Cinegéticos (IREC-CSIC-UCLM-JCCLM)SaBio. Instituto de Investigación en Recursos Cinegéticos (IREC-CSIC-UCLM-JCCLM)Veterinary AuthorityVeterinary AuthorityFood Chain Safety and Veterinary AuthorityClinical Laboratory and Center for Clinical Studies, Vetsuisse Faculty, University of ZurichDepartment of Parasitology and Zoology, University of Veterinary MedicineDepartment of Parasitology and Zoology, University of Veterinary MedicineDepartment of Parasitology and Zoology, University of Veterinary MedicineClinical Laboratory and Center for Clinical Studies, Vetsuisse Faculty, University of ZurichClinical Laboratory and Center for Clinical Studies, Vetsuisse Faculty, University of ZurichAbstract Background Hunting constitutes an important industry in Europe. However, data on the prevalence of vector-borne bacteria in large game animal species are lacking from several countries. Blood or spleen samples (239 and 270, respectively) were taken from red, fallow and roe deer, as well as from water buffaloes, mouflons and wild boars in Hungary, followed by DNA extraction and molecular analyses for Anaplasma phagocytophilum, haemoplasmas and rickettsiae. Results Based on blood samples, the prevalence rate of A. phagocytophilum infection was significantly higher in red deer (97.9%) than in fallow deer (72.7%) and roe deer (60%), and in all these compared to mouflons (6.3%). In addition, 39.2% of the spleen samples from wild boars were PCR positive for A. phagocytophilum, but none of the buffalos. Based on blood samples, the prevalence rates of both Mycoplasma wenyonii (Mw) and ‘Candidatus M. haemobos’ (CMh) infections were significantly higher in buffaloes (Mw: 91.2%; CMh: 73.3%) than in red deer (Mw: 64.6%; CMh: 45.8%), and in both of them compared to fallow deer (Mw: 30.3%; CMh: 9.1%) and roe deer (Mw: 20%; CMh: 1.5%). The prevalence of Mw and CMh infection significantly correlated with the body sizes of these hosts. Furthermore, Mw was significantly more prevalent than CMh in buffaloes, red and roe deer. Mycoplasma ovis was detected in mouflons, M. suis in wild boars, R. helvetica in one fallow deer and one mouflon, and an unidentified Rickettsia sp. in a fallow deer. Conclusions Forest-dwelling game animal species were found to be important carriers of A. phagocytophilum. In contrast, animals grazing grassland (i.e. buffaloes) were less likely to get infected with this Ixodes ricinus-borne pathogen. Water buffaloes, deer species, mouflons and wild boars harbored haemoplasmas that may affect domestic ungulates. Evaluated animals with larger body size had significantly higher prevalence of infection with haemoplasmas compared to smaller deer species. The above host species rarely carried rickettsiae.http://link.springer.com/article/10.1186/s12917-018-1403-6Anaplasma phagocytophilumMycoplasma wenyonii‘Candidatus Mycoplasma haemobos’Mycoplasma suisRickettsia helvetica |
| spellingShingle | Sándor Hornok László Sugár Isabel G. Fernández de Mera José de la Fuente Gábor Horváth Tibor Kovács Attila Micsutka Enikő Gönczi Barbara Flaisz Nóra Takács Róbert Farkas Marina L. Meli Regina Hofmann-Lehmann Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary BMC Veterinary Research Anaplasma phagocytophilum Mycoplasma wenyonii ‘Candidatus Mycoplasma haemobos’ Mycoplasma suis Rickettsia helvetica |
| title | Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary |
| title_full | Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary |
| title_fullStr | Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary |
| title_full_unstemmed | Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary |
| title_short | Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary |
| title_sort | tick and fly borne bacteria in ungulates the prevalence of anaplasma phagocytophilum haemoplasmas and rickettsiae in water buffalo and deer species in central europe hungary |
| topic | Anaplasma phagocytophilum Mycoplasma wenyonii ‘Candidatus Mycoplasma haemobos’ Mycoplasma suis Rickettsia helvetica |
| url | http://link.springer.com/article/10.1186/s12917-018-1403-6 |
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