Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish
Okadaic acid (OA) and the analogs of dinophysistoxin (DTX) are important members of diarrhetic shellfish poisoning (DSP) toxins. In this study, five OA-specific mAbs (monoclonal antibodies) were developed from five stable cells of hybridoma. The anti-OA mAb-2D7 showed high sensitivity to OA, the IC5...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
|
| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/09540105.2022.2115466 |
| _version_ | 1818472382386405376 |
|---|---|
| author | Ruirui Wang Hongli Qi Miao Liang Guoxiang Liao Fan Yang |
| author_facet | Ruirui Wang Hongli Qi Miao Liang Guoxiang Liao Fan Yang |
| author_sort | Ruirui Wang |
| collection | DOAJ |
| description | Okadaic acid (OA) and the analogs of dinophysistoxin (DTX) are important members of diarrhetic shellfish poisoning (DSP) toxins. In this study, five OA-specific mAbs (monoclonal antibodies) were developed from five stable cells of hybridoma. The anti-OA mAb-2D7 showed high sensitivity to OA, the IC50 of the antibody was 0.24 ng/mL, and its cross-reactivity was 91.6% with DTX-1 and 110.5% with DTX-2., In dcELISA, the IC50 was set at 0.182 ng/mL, and the detection limit was set at 0.023 ng/mL by using the anti-OA mAb-2D7. The level of OA recovered from spiked mussel samples was 2–50 ng/g, ranging from 97.6 ± 7.2% to 106.4 ± 9.8%. In contrast, the immunostrip assay with a limit of 5 ng/mL, conducted for detecting OA, was completed in 10 min. The mAb-based dcELISA and immunostrip assay developed were precise and sensitive enough to quickly assess OA, DTX-1, and DTX-2 in shellfish specimens. |
| first_indexed | 2024-04-14T04:06:38Z |
| format | Article |
| id | doaj.art-f527bf85c2e64138ad044c871089d984 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-04-14T04:06:38Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-f527bf85c2e64138ad044c871089d9842022-12-22T02:13:20ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432022-12-0133167769110.1080/09540105.2022.2115466Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfishRuirui Wang0Hongli Qi1Miao Liang2Guoxiang Liao3Fan Yang4National Marine Environmental Monitoring Center, Dalian, People’s Republic of ChinaFisheries College, The key lab of Tianjin aquatic ecology and aquaculture, Tianjin Agricultural University, Tianjin, People’s Republic of ChinaNational Marine Environmental Monitoring Center, Dalian, People’s Republic of ChinaNational Marine Environmental Monitoring Center, Dalian, People’s Republic of ChinaNational Marine Environmental Monitoring Center, Dalian, People’s Republic of ChinaOkadaic acid (OA) and the analogs of dinophysistoxin (DTX) are important members of diarrhetic shellfish poisoning (DSP) toxins. In this study, five OA-specific mAbs (monoclonal antibodies) were developed from five stable cells of hybridoma. The anti-OA mAb-2D7 showed high sensitivity to OA, the IC50 of the antibody was 0.24 ng/mL, and its cross-reactivity was 91.6% with DTX-1 and 110.5% with DTX-2., In dcELISA, the IC50 was set at 0.182 ng/mL, and the detection limit was set at 0.023 ng/mL by using the anti-OA mAb-2D7. The level of OA recovered from spiked mussel samples was 2–50 ng/g, ranging from 97.6 ± 7.2% to 106.4 ± 9.8%. In contrast, the immunostrip assay with a limit of 5 ng/mL, conducted for detecting OA, was completed in 10 min. The mAb-based dcELISA and immunostrip assay developed were precise and sensitive enough to quickly assess OA, DTX-1, and DTX-2 in shellfish specimens.https://www.tandfonline.com/doi/10.1080/09540105.2022.2115466Okadaic acidDcELISAcolloidal gold immunoassayshellfish |
| spellingShingle | Ruirui Wang Hongli Qi Miao Liang Guoxiang Liao Fan Yang Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish Food and Agricultural Immunology Okadaic acid DcELISA colloidal gold immunoassay shellfish |
| title | Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish |
| title_full | Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish |
| title_fullStr | Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish |
| title_full_unstemmed | Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish |
| title_short | Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish |
| title_sort | rapid enzyme linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish |
| topic | Okadaic acid DcELISA colloidal gold immunoassay shellfish |
| url | https://www.tandfonline.com/doi/10.1080/09540105.2022.2115466 |
| work_keys_str_mv | AT ruiruiwang rapidenzymelinkedimmunosorbentassayandcolloidalgoldimmunoassayforassessingokadaicacidanditsderivativesinshellfish AT hongliqi rapidenzymelinkedimmunosorbentassayandcolloidalgoldimmunoassayforassessingokadaicacidanditsderivativesinshellfish AT miaoliang rapidenzymelinkedimmunosorbentassayandcolloidalgoldimmunoassayforassessingokadaicacidanditsderivativesinshellfish AT guoxiangliao rapidenzymelinkedimmunosorbentassayandcolloidalgoldimmunoassayforassessingokadaicacidanditsderivativesinshellfish AT fanyang rapidenzymelinkedimmunosorbentassayandcolloidalgoldimmunoassayforassessingokadaicacidanditsderivativesinshellfish |