Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical Applications
The paper presents the results of the synthesis, a detailed kinetics study, and an investigation of the biological activity of silver nanoparticles (AgNPs) in aqueous solutions of N-reacetylated oligochitosan hydrochloride. UV–visible spectrophotometry and dynamic light scattering were employed to c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/12/2690 |
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| author | Ekaterina K. Urodkova Ol’ga Ya. Uryupina Vladimir E. Tikhonov Natalia E. Grammatikova Anastasia V. Bol’shakova Anna A. Sinelshchikova Alexandra I. Zvyagina Dmitry N. Khmelenin Elena S. Zhavoronok Ivan N. Senchikhin |
| author_facet | Ekaterina K. Urodkova Ol’ga Ya. Uryupina Vladimir E. Tikhonov Natalia E. Grammatikova Anastasia V. Bol’shakova Anna A. Sinelshchikova Alexandra I. Zvyagina Dmitry N. Khmelenin Elena S. Zhavoronok Ivan N. Senchikhin |
| author_sort | Ekaterina K. Urodkova |
| collection | DOAJ |
| description | The paper presents the results of the synthesis, a detailed kinetics study, and an investigation of the biological activity of silver nanoparticles (AgNPs) in aqueous solutions of N-reacetylated oligochitosan hydrochloride. UV–visible spectrophotometry and dynamic light scattering were employed to control silver ion reduction. The process was observed to follow a pseudo-first-order law. Transmission and scanning electron microscopy demonstrated that AgNPs ranging in size from 10 to 25 nm formed aggregates measuring 60 to 90 nm, with the aggregate surface coated by a 2–4 nm chitosan shell. X-ray microanalysis and powder X-ray diffractometry were used to study the phase composition, identifying two crystalline phases, nanocrystalline silver and AgCl, present in the dispersions. The antibacterial effect was assessed using the serial dilution method for dispersions with varying degrees of Ag<sup>+</sup> conversion. Nanodispersions exhibited significant activity against <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Bacillus cereus</i>, and <i>Staphylococcus aureus</i>. Interestingly, the activity did not appear to be heavily influenced by the presence of the AgCl phase or the concentration of Ag<sup>+</sup> ions. These synthesized dispersions hold promise for the development of materials tailored for biomedical applications. |
| first_indexed | 2024-03-08T20:27:35Z |
| format | Article |
| id | doaj.art-f5294b8ec6da442995e278e149dccabe |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-08T20:27:35Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-f5294b8ec6da442995e278e149dccabe2023-12-22T14:32:00ZengMDPI AGPharmaceutics1999-49232023-11-011512269010.3390/pharmaceutics15122690Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical ApplicationsEkaterina K. Urodkova0Ol’ga Ya. Uryupina1Vladimir E. Tikhonov2Natalia E. Grammatikova3Anastasia V. Bol’shakova4Anna A. Sinelshchikova5Alexandra I. Zvyagina6Dmitry N. Khmelenin7Elena S. Zhavoronok8Ivan N. Senchikhin9A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, 119991 Moscow, RussiaG.F. Gause Institute of New Antibiotics, 119021 Moscow, RussiaA.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, RussiaA.V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, 119333 Moscow, RussiaLomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaA.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, RussiaThe paper presents the results of the synthesis, a detailed kinetics study, and an investigation of the biological activity of silver nanoparticles (AgNPs) in aqueous solutions of N-reacetylated oligochitosan hydrochloride. UV–visible spectrophotometry and dynamic light scattering were employed to control silver ion reduction. The process was observed to follow a pseudo-first-order law. Transmission and scanning electron microscopy demonstrated that AgNPs ranging in size from 10 to 25 nm formed aggregates measuring 60 to 90 nm, with the aggregate surface coated by a 2–4 nm chitosan shell. X-ray microanalysis and powder X-ray diffractometry were used to study the phase composition, identifying two crystalline phases, nanocrystalline silver and AgCl, present in the dispersions. The antibacterial effect was assessed using the serial dilution method for dispersions with varying degrees of Ag<sup>+</sup> conversion. Nanodispersions exhibited significant activity against <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Bacillus cereus</i>, and <i>Staphylococcus aureus</i>. Interestingly, the activity did not appear to be heavily influenced by the presence of the AgCl phase or the concentration of Ag<sup>+</sup> ions. These synthesized dispersions hold promise for the development of materials tailored for biomedical applications.https://www.mdpi.com/1999-4923/15/12/2690chitosanoligochitosanhydrochloridesilver nanoparticlessurface functionalizationantimicrobial activity |
| spellingShingle | Ekaterina K. Urodkova Ol’ga Ya. Uryupina Vladimir E. Tikhonov Natalia E. Grammatikova Anastasia V. Bol’shakova Anna A. Sinelshchikova Alexandra I. Zvyagina Dmitry N. Khmelenin Elena S. Zhavoronok Ivan N. Senchikhin Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical Applications Pharmaceutics chitosan oligochitosan hydrochloride silver nanoparticles surface functionalization antimicrobial activity |
| title | Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical Applications |
| title_full | Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical Applications |
| title_fullStr | Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical Applications |
| title_full_unstemmed | Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical Applications |
| title_short | Formation Kinetics and Antimicrobial Activity of Silver Nanoparticle Dispersions Based on N-Reacetylated Oligochitosan Solutions for Biomedical Applications |
| title_sort | formation kinetics and antimicrobial activity of silver nanoparticle dispersions based on n reacetylated oligochitosan solutions for biomedical applications |
| topic | chitosan oligochitosan hydrochloride silver nanoparticles surface functionalization antimicrobial activity |
| url | https://www.mdpi.com/1999-4923/15/12/2690 |
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