JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in Drosophila
The fusion of epithelial sheets is an essential and conserved morphogenetic event that requires the maintenance of tissue continuity. This is secured by membrane-bound or diffusible signals that instruct the epithelial cells, in a coordinated fashion, to change shapes and adhesive properties and whe...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1034484/full |
| _version_ | 1797361402860535808 |
|---|---|
| author | Katerina Karkali Jose Carlos Pastor-Pareja Enrique Martin-Blanco |
| author_facet | Katerina Karkali Jose Carlos Pastor-Pareja Enrique Martin-Blanco |
| author_sort | Katerina Karkali |
| collection | DOAJ |
| description | The fusion of epithelial sheets is an essential and conserved morphogenetic event that requires the maintenance of tissue continuity. This is secured by membrane-bound or diffusible signals that instruct the epithelial cells, in a coordinated fashion, to change shapes and adhesive properties and when, how and where to move. Here we show that during Dorsal Closure (DC) in Drosophila, the Jun kinase (JNK) signaling pathway modulates integrins expression and ensures tissue endurance. An excess of JNK activity, as an outcome of a failure in the negative feedback implemented by the dual-specificity phosphatase Puckered (Puc), promotes the loss of integrins [the ß-subunit Myospheroid (Mys)] and amnioserosa detachment. Likewise, integrins signal back to the pathway to regulate the duration and strength of JNK activity. Mys is necessary for the regulation of JNK activity levels and in its absence, puc expression is downregulated and JNK activity increases. |
| first_indexed | 2024-03-08T15:53:11Z |
| format | Article |
| id | doaj.art-f52aa850997845a6bbcdb984e58237ea |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-08T15:53:11Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-f52aa850997845a6bbcdb984e58237ea2024-01-09T04:11:24ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2024-01-011110.3389/fcell.2023.10344841034484JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in DrosophilaKaterina Karkali0Jose Carlos Pastor-Pareja1Enrique Martin-Blanco2Instituto de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas (IBMB-CSIC), Barcelona, SpainInstituto de Neurociencias, Consejo Superior de Investigaciones Científicas (IN-CSIC), Alicante, SpainInstituto de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas (IBMB-CSIC), Barcelona, SpainThe fusion of epithelial sheets is an essential and conserved morphogenetic event that requires the maintenance of tissue continuity. This is secured by membrane-bound or diffusible signals that instruct the epithelial cells, in a coordinated fashion, to change shapes and adhesive properties and when, how and where to move. Here we show that during Dorsal Closure (DC) in Drosophila, the Jun kinase (JNK) signaling pathway modulates integrins expression and ensures tissue endurance. An excess of JNK activity, as an outcome of a failure in the negative feedback implemented by the dual-specificity phosphatase Puckered (Puc), promotes the loss of integrins [the ß-subunit Myospheroid (Mys)] and amnioserosa detachment. Likewise, integrins signal back to the pathway to regulate the duration and strength of JNK activity. Mys is necessary for the regulation of JNK activity levels and in its absence, puc expression is downregulated and JNK activity increases.https://www.frontiersin.org/articles/10.3389/fcell.2023.1034484/fullDrosophilaJNKpuckeredintegrindorsal closureJNK sensor |
| spellingShingle | Katerina Karkali Jose Carlos Pastor-Pareja Enrique Martin-Blanco JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in Drosophila Frontiers in Cell and Developmental Biology Drosophila JNK puckered integrin dorsal closure JNK sensor |
| title | JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in Drosophila |
| title_full | JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in Drosophila |
| title_fullStr | JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in Drosophila |
| title_full_unstemmed | JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in Drosophila |
| title_short | JNK signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in Drosophila |
| title_sort | jnk signaling and integrins cooperate to maintain cell adhesion during epithelial fusion in drosophila |
| topic | Drosophila JNK puckered integrin dorsal closure JNK sensor |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1034484/full |
| work_keys_str_mv | AT katerinakarkali jnksignalingandintegrinscooperatetomaintaincelladhesionduringepithelialfusionindrosophila AT josecarlospastorpareja jnksignalingandintegrinscooperatetomaintaincelladhesionduringepithelialfusionindrosophila AT enriquemartinblanco jnksignalingandintegrinscooperatetomaintaincelladhesionduringepithelialfusionindrosophila |