POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASE
<p><strong>Aim. </strong>To evaluate the functional activity of platelets by the optical standard aggregatometry and induced flow cytofluorometry during antiplatelet therapy with clopidogrel and acetylsalicylic acid (ASA).</p><p><strong>Material and methods. </...
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| Format: | Article |
| Language: | English |
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Столичная издательская компания
2015-09-01
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| Series: | Рациональная фармакотерапия в кардиологии |
| Subjects: | |
| Online Access: | http://www.rpcardio.ru/jour/article/view/38 |
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| author | O. V. Sirotkina A. B. Laskovets A. S. Lipunova L. B. Gaykovaya S. A. Boldueva T. V. Vavilova |
| author_facet | O. V. Sirotkina A. B. Laskovets A. S. Lipunova L. B. Gaykovaya S. A. Boldueva T. V. Vavilova |
| author_sort | O. V. Sirotkina |
| collection | DOAJ |
| description | <p><strong>Aim. </strong>To evaluate the functional activity of platelets by the optical standard aggregatometry and induced flow cytofluorometry during antiplatelet therapy with clopidogrel and acetylsalicylic acid (ASA).</p><p><strong>Material and methods. </strong>The evaluation of platelet function in 30 patients with ischemic heart disease treated with dual antiplatelet therapy (ASA and clopidogrel) was performed by two methods: the standard photometric method with the assessment of degree of light transmission at the maximum point and by the original method of induced flow cytofluorometry with the platelet activity evaluation by changing of glycoprotein (GP) IIb/IIIa receptor level and the expression of P-selectin before and after ADP induction.</p><p><strong>Results. </strong>Increase in platelet functional activity was detected in patients with ASA monotherapy at initial evaluation by both induced flow cytofluorometry and standard photometric method. After one month dual antiplatelet therapy platelet function significantly decreased according to standard photometric method (from 61.4Ѓ}3.6 to 45.9Ѓ}3.7; p<0.05; the induction of 2.5 mM ADP), as well as according to flow cytofluorometry with changing of GP IIb/IIIa receptor level (from 12.2Ѓ}0.8% to 5.2Ѓ}0.8%; p<0.05) and the expression of P-selectin (from 70.5Ѓ}5.9% to 57.4Ѓ}5.9%; p<0.05).</p><p><strong>Conclusion. </strong>The combined use of laboratory methods to assess platelet function (traditional and high-tech) provides cardiologist with additional tool for assessing the effectiveness of antiplatelet therapy in patients with ischemic heart disease.</p> |
| first_indexed | 2024-03-12T08:27:20Z |
| format | Article |
| id | doaj.art-f52b9afaaff64fdea64aede5b117d18a |
| institution | Directory Open Access Journal |
| issn | 1819-6446 2225-3653 |
| language | English |
| last_indexed | 2024-03-12T08:27:20Z |
| publishDate | 2015-09-01 |
| publisher | Столичная издательская компания |
| record_format | Article |
| series | Рациональная фармакотерапия в кардиологии |
| spelling | doaj.art-f52b9afaaff64fdea64aede5b117d18a2023-09-02T18:03:46ZengСтоличная издательская компанияРациональная фармакотерапия в кардиологии1819-64462225-36532015-09-0191313410.1234/1819-6446-2013-1-31-3438POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASEO. V. Sirotkina0A. B. Laskovets1A. S. Lipunova2L. B. Gaykovaya3S. A. Boldueva4T. V. Vavilova5Северо-Западный государственный медицинский университет им. И.И.Мечникова, Санкт-Петербург; Петербургский институт ядерной физики им. Б.П.Константинова, Гатчина, Ленинградская обл.Северо-Западный государственный медицинский университет им. И.И.Мечникова, Санкт-ПетербургСеверо-Западный государственный медицинский университет им. И.И.Мечникова, Санкт-ПетербургСеверо-Западный государственный медицинский университет им. И.И.Мечникова, Санкт-ПетербургСеверо-Западный государственный медицинский университет им. И.И.Мечникова, Санкт-ПетербургСеверо-Западный государственный медицинский университет им. И.И.Мечникова, Санкт-Петербург<p><strong>Aim. </strong>To evaluate the functional activity of platelets by the optical standard aggregatometry and induced flow cytofluorometry during antiplatelet therapy with clopidogrel and acetylsalicylic acid (ASA).</p><p><strong>Material and methods. </strong>The evaluation of platelet function in 30 patients with ischemic heart disease treated with dual antiplatelet therapy (ASA and clopidogrel) was performed by two methods: the standard photometric method with the assessment of degree of light transmission at the maximum point and by the original method of induced flow cytofluorometry with the platelet activity evaluation by changing of glycoprotein (GP) IIb/IIIa receptor level and the expression of P-selectin before and after ADP induction.</p><p><strong>Results. </strong>Increase in platelet functional activity was detected in patients with ASA monotherapy at initial evaluation by both induced flow cytofluorometry and standard photometric method. After one month dual antiplatelet therapy platelet function significantly decreased according to standard photometric method (from 61.4Ѓ}3.6 to 45.9Ѓ}3.7; p<0.05; the induction of 2.5 mM ADP), as well as according to flow cytofluorometry with changing of GP IIb/IIIa receptor level (from 12.2Ѓ}0.8% to 5.2Ѓ}0.8%; p<0.05) and the expression of P-selectin (from 70.5Ѓ}5.9% to 57.4Ѓ}5.9%; p<0.05).</p><p><strong>Conclusion. </strong>The combined use of laboratory methods to assess platelet function (traditional and high-tech) provides cardiologist with additional tool for assessing the effectiveness of antiplatelet therapy in patients with ischemic heart disease.</p>http://www.rpcardio.ru/jour/article/view/38клопидогрелантиагрегантная терапияGP IIb/IIIa рецепторыР-селектининдуцированная проточная тромбоцитометрия |
| spellingShingle | O. V. Sirotkina A. B. Laskovets A. S. Lipunova L. B. Gaykovaya S. A. Boldueva T. V. Vavilova POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASE Рациональная фармакотерапия в кардиологии клопидогрел антиагрегантная терапия GP IIb/IIIa рецепторы Р-селектин индуцированная проточная тромбоцитометрия |
| title | POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASE |
| title_full | POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASE |
| title_fullStr | POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASE |
| title_full_unstemmed | POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASE |
| title_short | POSSIBILITIES OF CLINICAL LABORATORY EVALUATION OF ANTIPLATELET THERAPY EFFECTIVENESS IN PATIENTS WITH ISCHEMIC HEART DISEASE |
| title_sort | possibilities of clinical laboratory evaluation of antiplatelet therapy effectiveness in patients with ischemic heart disease |
| topic | клопидогрел антиагрегантная терапия GP IIb/IIIa рецепторы Р-селектин индуцированная проточная тромбоцитометрия |
| url | http://www.rpcardio.ru/jour/article/view/38 |
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