8-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine Model
The effects of 8-Methoxypsoralen plus ultraviolet A (PUVA) or ultraviolet B (UVB) alone on imiquimod-induced psoriasis were examined in a mouse model. Mouse skin was treated with repetitive sub-phototoxic doses of PUVA or UVB before or during the induction of toll-like receptor 7/8 activation and ps...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Medical Journals Sweden
2018-03-01
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| Series: | Acta Dermato-Venereologica |
| Subjects: | |
| Online Access: |
https://www.medicaljournals.se/acta/content/html/10.2340/00015555-2905
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| _version_ | 1818907159218356224 |
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| author | Nitesh Shirsath Karin Wagner Simone Tangermann Michaela Schlederer Christian Ringel Lukas Kenner Bernhard Brüne Peter Wolf |
| author_facet | Nitesh Shirsath Karin Wagner Simone Tangermann Michaela Schlederer Christian Ringel Lukas Kenner Bernhard Brüne Peter Wolf |
| author_sort | Nitesh Shirsath |
| collection | DOAJ |
| description | The effects of 8-Methoxypsoralen plus ultraviolet A (PUVA) or ultraviolet B (UVB) alone on imiquimod-induced psoriasis were examined in a mouse model. Mouse skin was treated with repetitive sub-phototoxic doses of PUVA or UVB before or during the induction of toll-like receptor 7/8 activation and psoriasis through the application of imiquimod. PUVA, to a greater degree than UVB, suppressed the established imiquimod-induced psoriatic phenotype, but pretreatment with PUVA prior to administration of imiquimod also reduced the susceptibility of murine skin to respond to imiquimod to a greater degree than did pretreatment with UVB. PUVA downregulated baseline levels of miRNA27a and 29a, as well as interferon-γ, interleukin-17 and -9, cytokines, which drive psoriatic inflammation. Microarray analysis showed enrichment of senescence pathway genes linked to upregulation of p16/p21 proteins after PUVA pretreatment. However, the anti-psoriatic effect of PUVA was lost when there was an interval of 7 days between final exposure to PUVA and the start of administration of imiquimod. This indicated that (UVB and) PUVA diminished imiquimod-induced established psoriatic inflammation, but also primed the skin in favour of a reduced responsiveness to toll-like receptor activation. |
| first_indexed | 2024-12-19T21:50:41Z |
| format | Article |
| id | doaj.art-f52ce1cad1b3494f993bb480f16be59c |
| institution | Directory Open Access Journal |
| issn | 0001-5555 1651-2057 |
| language | English |
| last_indexed | 2024-12-19T21:50:41Z |
| publishDate | 2018-03-01 |
| publisher | Medical Journals Sweden |
| record_format | Article |
| series | Acta Dermato-Venereologica |
| spelling | doaj.art-f52ce1cad1b3494f993bb480f16be59c2022-12-21T20:04:25ZengMedical Journals SwedenActa Dermato-Venereologica0001-55551651-20572018-03-0198657658410.2340/00015555-290551778-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine ModelNitesh Shirsath0Karin WagnerSimone TangermannMichaela SchledererChristian RingelLukas KennerBernhard BrünePeter Wolf Research Unit for Photodermatology, Department of Dermatology and Venereology, Medical University of Graz, AT-8036 Graz, Austria. The effects of 8-Methoxypsoralen plus ultraviolet A (PUVA) or ultraviolet B (UVB) alone on imiquimod-induced psoriasis were examined in a mouse model. Mouse skin was treated with repetitive sub-phototoxic doses of PUVA or UVB before or during the induction of toll-like receptor 7/8 activation and psoriasis through the application of imiquimod. PUVA, to a greater degree than UVB, suppressed the established imiquimod-induced psoriatic phenotype, but pretreatment with PUVA prior to administration of imiquimod also reduced the susceptibility of murine skin to respond to imiquimod to a greater degree than did pretreatment with UVB. PUVA downregulated baseline levels of miRNA27a and 29a, as well as interferon-γ, interleukin-17 and -9, cytokines, which drive psoriatic inflammation. Microarray analysis showed enrichment of senescence pathway genes linked to upregulation of p16/p21 proteins after PUVA pretreatment. However, the anti-psoriatic effect of PUVA was lost when there was an interval of 7 days between final exposure to PUVA and the start of administration of imiquimod. This indicated that (UVB and) PUVA diminished imiquimod-induced established psoriatic inflammation, but also primed the skin in favour of a reduced responsiveness to toll-like receptor activation. https://www.medicaljournals.se/acta/content/html/10.2340/00015555-2905 psoriasisPUVAUVBimiquimodinterleukin-9,senescence |
| spellingShingle | Nitesh Shirsath Karin Wagner Simone Tangermann Michaela Schlederer Christian Ringel Lukas Kenner Bernhard Brüne Peter Wolf 8-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine Model Acta Dermato-Venereologica psoriasis PUVA UVB imiquimod interleukin-9,senescence |
| title | 8-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine Model |
| title_full | 8-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine Model |
| title_fullStr | 8-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine Model |
| title_full_unstemmed | 8-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine Model |
| title_short | 8-Methoxypsoralen Plus Ultraviolet A Reduces the Psoriatic Response to Imiquimod in a Murine Model |
| title_sort | 8 methoxypsoralen plus ultraviolet a reduces the psoriatic response to imiquimod in a murine model |
| topic | psoriasis PUVA UVB imiquimod interleukin-9,senescence |
| url |
https://www.medicaljournals.se/acta/content/html/10.2340/00015555-2905
|
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