Ligand-competent fractalkine receptor is expressed on exosomes
Expression of chemokine receptor CX3CR1 is reportedly restricted to several cell types including natural killer cells, cytotoxic T cells, monocytes, and macrophages. However, its expression and function on exosomes, which are nanosized extracellular vesicles known to act as mediators of intercellula...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-07-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580821000261 |
| _version_ | 1818865289529393152 |
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| author | Eun Jeong Park Phyoe Kyawe Myint Michael G. Appiah Patsorn Worawattananutai Janjira Inprasit Onmanee Prajuabjinda Zay Yar Soe Arong Gaowa Eiji Kawamoto Motomu Shimaoka |
| author_facet | Eun Jeong Park Phyoe Kyawe Myint Michael G. Appiah Patsorn Worawattananutai Janjira Inprasit Onmanee Prajuabjinda Zay Yar Soe Arong Gaowa Eiji Kawamoto Motomu Shimaoka |
| author_sort | Eun Jeong Park |
| collection | DOAJ |
| description | Expression of chemokine receptor CX3CR1 is reportedly restricted to several cell types including natural killer cells, cytotoxic T cells, monocytes, and macrophages. However, its expression and function on exosomes, which are nanosized extracellular vesicles known to act as mediators of intercellular communications, remain unclear. Here, we investigated CX3CR1 expression on exosomes isolated from various cell types. Although we found that all the exosomes tested in our study highly expressed CX3CR1, this chemokine receptor was expressed only inside, but barely on, their source cells. Moreover, exosomal CX3CR1 was capable of binding soluble CX3CL1. Therefore, our study suggests that CX3CR1 is a novel and ligand-competent exosome receptor. |
| first_indexed | 2024-12-19T10:45:11Z |
| format | Article |
| id | doaj.art-f52d51bb3b4944babdcd6a387db3b48b |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-19T10:45:11Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-f52d51bb3b4944babdcd6a387db3b48b2022-12-21T20:25:18ZengElsevierBiochemistry and Biophysics Reports2405-58082021-07-0126100932Ligand-competent fractalkine receptor is expressed on exosomesEun Jeong Park0Phyoe Kyawe Myint1Michael G. Appiah2Patsorn Worawattananutai3Janjira Inprasit4Onmanee Prajuabjinda5Zay Yar Soe6Arong Gaowa7Eiji Kawamoto8Motomu Shimaoka9Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan; Corresponding author.Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan; Department of Emergency and Disaster Medicine, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan; Corresponding author.Expression of chemokine receptor CX3CR1 is reportedly restricted to several cell types including natural killer cells, cytotoxic T cells, monocytes, and macrophages. However, its expression and function on exosomes, which are nanosized extracellular vesicles known to act as mediators of intercellular communications, remain unclear. Here, we investigated CX3CR1 expression on exosomes isolated from various cell types. Although we found that all the exosomes tested in our study highly expressed CX3CR1, this chemokine receptor was expressed only inside, but barely on, their source cells. Moreover, exosomal CX3CR1 was capable of binding soluble CX3CL1. Therefore, our study suggests that CX3CR1 is a novel and ligand-competent exosome receptor.http://www.sciencedirect.com/science/article/pii/S2405580821000261ExosomeChemokineFractalkineChemokine receptorCX3CR1 |
| spellingShingle | Eun Jeong Park Phyoe Kyawe Myint Michael G. Appiah Patsorn Worawattananutai Janjira Inprasit Onmanee Prajuabjinda Zay Yar Soe Arong Gaowa Eiji Kawamoto Motomu Shimaoka Ligand-competent fractalkine receptor is expressed on exosomes Biochemistry and Biophysics Reports Exosome Chemokine Fractalkine Chemokine receptor CX3CR1 |
| title | Ligand-competent fractalkine receptor is expressed on exosomes |
| title_full | Ligand-competent fractalkine receptor is expressed on exosomes |
| title_fullStr | Ligand-competent fractalkine receptor is expressed on exosomes |
| title_full_unstemmed | Ligand-competent fractalkine receptor is expressed on exosomes |
| title_short | Ligand-competent fractalkine receptor is expressed on exosomes |
| title_sort | ligand competent fractalkine receptor is expressed on exosomes |
| topic | Exosome Chemokine Fractalkine Chemokine receptor CX3CR1 |
| url | http://www.sciencedirect.com/science/article/pii/S2405580821000261 |
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