Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus
ABSTRACT The objective of this work was to characterize reference bacteria strains, belonging to the genus Bacillus and species of correlated genera, by simplified morphological and biochemical methods. The morphological characterization is based on the aspects of the colonies, as well as cytomorpho...
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| Format: | Article |
| Language: | English |
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Universidade Federal De Viçosa
2023-06-01
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| Series: | Revista Ceres |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2023000300091&tlng=en |
| _version_ | 1797791557040996352 |
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| author | Gabriela Teodoro Rocha Sandro Coelho Linhares Montalvão Paulo Roberto Martins Queiroz Marcelo Rodrigues Berçot Ana Cristina Meneses Mendes Gomes Rose Gomes Monnerat |
| author_facet | Gabriela Teodoro Rocha Sandro Coelho Linhares Montalvão Paulo Roberto Martins Queiroz Marcelo Rodrigues Berçot Ana Cristina Meneses Mendes Gomes Rose Gomes Monnerat |
| author_sort | Gabriela Teodoro Rocha |
| collection | DOAJ |
| description | ABSTRACT The objective of this work was to characterize reference bacteria strains, belonging to the genus Bacillus and species of correlated genera, by simplified morphological and biochemical methods. The morphological characterization is based on the aspects of the colonies, as well as cytomorphology of the species, by optical and scanning microscopy. For biochemical characterization, the sensitivity test to antimicrobials by disk-diffusion is performed. Moreover, the strains were characterized by extracting intracellular proteins. Characteristics such as shape, color, and consistency of the colonies, in addition to the type of spore and production of protein crystals were determinants for the morphological characterization of these species. The antibiogram revealed high resistance to β-lactam group antibiotics, in species of Bacillus cereus s.l group. In Bacillus subtilis s.l. group there was high susceptibility to antibiograms, mainly for species of B. subtilis. The protein profile provided specific protein patterns for some species, mainly bands of 130 e 65 kDa for B. thuringiensis, 140 e 130 kDa for Lysinibacillus sphaericus, and 115 kDa for Brevibacillus laterosporus. Our results showed that the morphological and biochemical characterizations, provided a simple identification, with easy interpretation, and low cost. |
| first_indexed | 2024-03-13T02:20:33Z |
| format | Article |
| id | doaj.art-f5323d2c69c144c0821134a0f4df6fbe |
| institution | Directory Open Access Journal |
| issn | 2177-3491 |
| language | English |
| last_indexed | 2024-03-13T02:20:33Z |
| publishDate | 2023-06-01 |
| publisher | Universidade Federal De Viçosa |
| record_format | Article |
| series | Revista Ceres |
| spelling | doaj.art-f5323d2c69c144c0821134a0f4df6fbe2023-06-30T10:47:14ZengUniversidade Federal De ViçosaRevista Ceres2177-34912023-06-017039110410.1590/0034-737x202370030010Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and BrevibacillusGabriela Teodoro Rochahttps://orcid.org/0000-0001-7313-8922Sandro Coelho Linhares MontalvãoPaulo Roberto Martins QueirozMarcelo Rodrigues BerçotAna Cristina Meneses Mendes GomesRose Gomes MonneratABSTRACT The objective of this work was to characterize reference bacteria strains, belonging to the genus Bacillus and species of correlated genera, by simplified morphological and biochemical methods. The morphological characterization is based on the aspects of the colonies, as well as cytomorphology of the species, by optical and scanning microscopy. For biochemical characterization, the sensitivity test to antimicrobials by disk-diffusion is performed. Moreover, the strains were characterized by extracting intracellular proteins. Characteristics such as shape, color, and consistency of the colonies, in addition to the type of spore and production of protein crystals were determinants for the morphological characterization of these species. The antibiogram revealed high resistance to β-lactam group antibiotics, in species of Bacillus cereus s.l group. In Bacillus subtilis s.l. group there was high susceptibility to antibiograms, mainly for species of B. subtilis. The protein profile provided specific protein patterns for some species, mainly bands of 130 e 65 kDa for B. thuringiensis, 140 e 130 kDa for Lysinibacillus sphaericus, and 115 kDa for Brevibacillus laterosporus. Our results showed that the morphological and biochemical characterizations, provided a simple identification, with easy interpretation, and low cost.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2023000300091&tlng=enhome productionBacillus thuringiensisprotein profileantibiogram |
| spellingShingle | Gabriela Teodoro Rocha Sandro Coelho Linhares Montalvão Paulo Roberto Martins Queiroz Marcelo Rodrigues Berçot Ana Cristina Meneses Mendes Gomes Rose Gomes Monnerat Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus Revista Ceres home production Bacillus thuringiensis protein profile antibiogram |
| title | Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus |
| title_full | Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus |
| title_fullStr | Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus |
| title_full_unstemmed | Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus |
| title_short | Morphological and biochemical characterization of bacterial species of Bacillus, Lysinibacillus and Brevibacillus |
| title_sort | morphological and biochemical characterization of bacterial species of bacillus lysinibacillus and brevibacillus |
| topic | home production Bacillus thuringiensis protein profile antibiogram |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2023000300091&tlng=en |
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