CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing
Abstract Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ ...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16004-w |
| _version_ | 1818196633416892416 |
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| author | Kyra L. Feuer Marah H. Wahbeh Christian Yovo Eman Rabie Anh-Thu N. Lam Sara Abdollahi Lindsay J. Young Bailey Rike Akul Umamageswaran Dimitrios Avramopoulos |
| author_facet | Kyra L. Feuer Marah H. Wahbeh Christian Yovo Eman Rabie Anh-Thu N. Lam Sara Abdollahi Lindsay J. Young Bailey Rike Akul Umamageswaran Dimitrios Avramopoulos |
| author_sort | Kyra L. Feuer |
| collection | DOAJ |
| description | Abstract Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing. |
| first_indexed | 2024-12-12T01:37:11Z |
| format | Article |
| id | doaj.art-f5326552857e4db0aa072037c3580d5b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T01:37:11Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f5326552857e4db0aa072037c3580d5b2022-12-22T00:42:48ZengNature PortfolioScientific Reports2045-23222022-07-011211410.1038/s41598-022-16004-wCRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editingKyra L. Feuer0Marah H. Wahbeh1Christian Yovo2Eman Rabie3Anh-Thu N. Lam4Sara Abdollahi5Lindsay J. Young6Bailey Rike7Akul Umamageswaran8Dimitrios Avramopoulos9McKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMedical Molecular Genetics Department, Human Genetics and Genome Research Division, National Research CentreMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineMcKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of MedicineAbstract Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.https://doi.org/10.1038/s41598-022-16004-w |
| spellingShingle | Kyra L. Feuer Marah H. Wahbeh Christian Yovo Eman Rabie Anh-Thu N. Lam Sara Abdollahi Lindsay J. Young Bailey Rike Akul Umamageswaran Dimitrios Avramopoulos CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing Scientific Reports |
| title | CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing |
| title_full | CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing |
| title_fullStr | CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing |
| title_full_unstemmed | CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing |
| title_short | CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing |
| title_sort | crispr del rei a simple flexible and efficient pipeline for scarless genome editing |
| url | https://doi.org/10.1038/s41598-022-16004-w |
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