CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Abstract Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ ...

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Bibliographic Details
Main Authors: Kyra L. Feuer, Marah H. Wahbeh, Christian Yovo, Eman Rabie, Anh-Thu N. Lam, Sara Abdollahi, Lindsay J. Young, Bailey Rike, Akul Umamageswaran, Dimitrios Avramopoulos
Format: Article
Language:English
Published: Nature Portfolio 2022-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-16004-w

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https://doi.org/10.1038/s41598-022-16004-w

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