Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo
Mammary tumors affect intact and elderly female dogs, and almost 50% of these cases are malignant. Cell culture offers a promising preclinical model to study this disease and creates the opportunity to deposit cell lines at a cell bank to allow greater assay reproducibility and more reliable validat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.583874/full |
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| author | Patrícia de Faria Lainetti Andressa Brandi Antonio Fernando Leis Filho Maria Carolina Mangini Prado Priscila Emiko Kobayashi Renée Laufer-Amorim Carlos Eduardo Fonseca-Alves Carlos Eduardo Fonseca-Alves |
| author_facet | Patrícia de Faria Lainetti Andressa Brandi Antonio Fernando Leis Filho Maria Carolina Mangini Prado Priscila Emiko Kobayashi Renée Laufer-Amorim Carlos Eduardo Fonseca-Alves Carlos Eduardo Fonseca-Alves |
| author_sort | Patrícia de Faria Lainetti |
| collection | DOAJ |
| description | Mammary tumors affect intact and elderly female dogs, and almost 50% of these cases are malignant. Cell culture offers a promising preclinical model to study this disease and creates the opportunity to deposit cell lines at a cell bank to allow greater assay reproducibility and more reliable validation of the results. Another important aspect is the possibility of establishing models and improving our understanding of tumor characteristics, such as vasculogenic mimicry. Because of the importance of cancer cell lines in preclinical models, the present study established and characterized primary cell lines from canine mammary gland tumors. Cell cultures were evaluated for morphology, phenotype, vasculogenic mimicry (VM), and tumorigenicity abilities. We collected 17 primary mammary carcinoma and three metastases and obtained satisfactory results from 10 samples. The cells were transplanted to a xenograft model. All cell lines exhibited a spindle-shaped or polygonal morphology and expressed concomitant pancytokeratin and cytokeratin 8/18. Four cell lines had vasculogenic mimicry ability in vitro, and two cell lines showed in vivo tumorigenicity and VM in the xenotransplanted tumor. Cellular characterization will help create a database to increase our knowledge of mammary carcinomas in dogs, including studies of tumor behavior and the identification of new therapeutic targets. |
| first_indexed | 2024-12-13T17:41:13Z |
| format | Article |
| id | doaj.art-f5371948eeba48e89817f8c92e5d06bd |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-13T17:41:13Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-f5371948eeba48e89817f8c92e5d06bd2022-12-21T23:36:45ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-10-01710.3389/fvets.2020.583874583874Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivoPatrícia de Faria Lainetti0Andressa Brandi1Antonio Fernando Leis Filho2Maria Carolina Mangini Prado3Priscila Emiko Kobayashi4Renée Laufer-Amorim5Carlos Eduardo Fonseca-Alves6Carlos Eduardo Fonseca-Alves7School of Veterinary Medicine and Animal Science, São Paulo State University—UNESP, Botucatu, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University—UNESP, Botucatu, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University—UNESP, Botucatu, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University—UNESP, Botucatu, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University—UNESP, Botucatu, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University—UNESP, Botucatu, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University—UNESP, Botucatu, BrazilInstitute of Health Sciences, Universidade Paulista-UNIP, Bauru, BrazilMammary tumors affect intact and elderly female dogs, and almost 50% of these cases are malignant. Cell culture offers a promising preclinical model to study this disease and creates the opportunity to deposit cell lines at a cell bank to allow greater assay reproducibility and more reliable validation of the results. Another important aspect is the possibility of establishing models and improving our understanding of tumor characteristics, such as vasculogenic mimicry. Because of the importance of cancer cell lines in preclinical models, the present study established and characterized primary cell lines from canine mammary gland tumors. Cell cultures were evaluated for morphology, phenotype, vasculogenic mimicry (VM), and tumorigenicity abilities. We collected 17 primary mammary carcinoma and three metastases and obtained satisfactory results from 10 samples. The cells were transplanted to a xenograft model. All cell lines exhibited a spindle-shaped or polygonal morphology and expressed concomitant pancytokeratin and cytokeratin 8/18. Four cell lines had vasculogenic mimicry ability in vitro, and two cell lines showed in vivo tumorigenicity and VM in the xenotransplanted tumor. Cellular characterization will help create a database to increase our knowledge of mammary carcinomas in dogs, including studies of tumor behavior and the identification of new therapeutic targets.https://www.frontiersin.org/articles/10.3389/fvets.2020.583874/fullfemale dogcell culturemammary tumorveterinaryoncology |
| spellingShingle | Patrícia de Faria Lainetti Andressa Brandi Antonio Fernando Leis Filho Maria Carolina Mangini Prado Priscila Emiko Kobayashi Renée Laufer-Amorim Carlos Eduardo Fonseca-Alves Carlos Eduardo Fonseca-Alves Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo Frontiers in Veterinary Science female dog cell culture mammary tumor veterinary oncology |
| title | Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo |
| title_full | Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo |
| title_fullStr | Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo |
| title_full_unstemmed | Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo |
| title_short | Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo |
| title_sort | establishment and characterization of canine mammary gland carcinoma cell lines with vasculogenic mimicry ability in vitro and in vivo |
| topic | female dog cell culture mammary tumor veterinary oncology |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2020.583874/full |
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