Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer
BACKGROUND: Parkia speciosa seeds have been reported to have an anticancer property due to the presence of various antioxidant compounds. Since the potential uses of P. speciosa for the tongue cancer has not been clearly disclosed, we conducted a study to investigate anticancer properties of P. spec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Secretariat of The Indonesian Biomedical Journal
2022-06-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/1800 |
| _version_ | 1811344402505793536 |
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| author | Erlina Sih Mahanani Ikhsan Nur Arifin Arya Nur Ihsan Yusrina Lukitasari Ferry Sandra |
| author_facet | Erlina Sih Mahanani Ikhsan Nur Arifin Arya Nur Ihsan Yusrina Lukitasari Ferry Sandra |
| author_sort | Erlina Sih Mahanani |
| collection | DOAJ |
| description | BACKGROUND: Parkia speciosa seeds have been reported to have an anticancer property due to the presence of various antioxidant compounds. Since the potential uses of P. speciosa for the tongue cancer has not been clearly disclosed, we conducted a study to investigate anticancer properties of P. speciosa seed ethanol extract (PSSEE) as well as its effect on cardiac cells.
METHODS: Tongue cancer rat model were treated with/without Doxorubicin and various concentrations of PSSEE. After treatment, tongue and heart samples were collected and processed further for histological examinations. Tongue epithelium thickness and damaged heart tissues was observed by HE staining, while tongue cancer cell proliferation was assessed by Ki-67 immunohistochemistry. Analyses were performed under an upright light microscope to measure tongue epithelium thickness, state of cancer cell proliferation, and degree of heart tissue damage.
RESULTS: Addition of 400 mg/kg body weight (BW) PSSEE to 4.6 mg/kg BW Doxorubicin reduced the average tongue epithelial thickness and Ki-67+ cells number. Upon addition of PSSEE to Doxorubicin, the damage of heart tissue was reduced in a concentration dependent manner. Among all groups, the group of tongue cancer treated with 4.6 mg/kg BW Doxorubicin and 400 mg/kg BW PSSEE had the lowest percentage as well as the lowest degree of heart tissue damage.
CONCLUSION: Since addition of PSSEE to Doxorubicin reduced epithelial thickness, number of Ki-67+ cells and heart tissue damage, PSSEE could be a potential co-chemotherapeutic agent for Doxorubicin toward tongue cancer.
KEYWORDS: Parkia speciosa, Doxorubicin, tongue cancer, epithelial thickness, Ki-67, cardiotoxicity, co-chemotherapy |
| first_indexed | 2024-04-13T19:46:47Z |
| format | Article |
| id | doaj.art-f5394b59850044c5a5030229ba83c0ac |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-04-13T19:46:47Z |
| publishDate | 2022-06-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj.art-f5394b59850044c5a5030229ba83c0ac2022-12-22T02:32:43ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792022-06-011421869210.18585/inabj.v14i2.1800460Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue CancerErlina Sih Mahanani0Ikhsan Nur Arifin1Arya Nur Ihsan2Yusrina Lukitasari3Ferry Sandra4Department of Dental Biomedical, School of Dentistry, Universitas Muhammadiyah Yogyakarta, Jl. Brawijaya, Kasihan, Bantul, Yogyakarta 55183Dental Hospital of Universitas Muhammadiyah Yogyakarta, Jl. HOS Cokroaminoto No.17, Pakuncen, Yogyakarta 55252Dental Hospital of Universitas Muhammadiyah Yogyakarta, Jl. HOS Cokroaminoto No.17, Pakuncen, Yogyakarta 55252Dental Hospital of Universitas Muhammadiyah Yogyakarta, Jl. HOS Cokroaminoto No.17, Pakuncen, Yogyakarta 55252Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, JakartaBACKGROUND: Parkia speciosa seeds have been reported to have an anticancer property due to the presence of various antioxidant compounds. Since the potential uses of P. speciosa for the tongue cancer has not been clearly disclosed, we conducted a study to investigate anticancer properties of P. speciosa seed ethanol extract (PSSEE) as well as its effect on cardiac cells. METHODS: Tongue cancer rat model were treated with/without Doxorubicin and various concentrations of PSSEE. After treatment, tongue and heart samples were collected and processed further for histological examinations. Tongue epithelium thickness and damaged heart tissues was observed by HE staining, while tongue cancer cell proliferation was assessed by Ki-67 immunohistochemistry. Analyses were performed under an upright light microscope to measure tongue epithelium thickness, state of cancer cell proliferation, and degree of heart tissue damage. RESULTS: Addition of 400 mg/kg body weight (BW) PSSEE to 4.6 mg/kg BW Doxorubicin reduced the average tongue epithelial thickness and Ki-67+ cells number. Upon addition of PSSEE to Doxorubicin, the damage of heart tissue was reduced in a concentration dependent manner. Among all groups, the group of tongue cancer treated with 4.6 mg/kg BW Doxorubicin and 400 mg/kg BW PSSEE had the lowest percentage as well as the lowest degree of heart tissue damage. CONCLUSION: Since addition of PSSEE to Doxorubicin reduced epithelial thickness, number of Ki-67+ cells and heart tissue damage, PSSEE could be a potential co-chemotherapeutic agent for Doxorubicin toward tongue cancer. KEYWORDS: Parkia speciosa, Doxorubicin, tongue cancer, epithelial thickness, Ki-67, cardiotoxicity, co-chemotherapyhttps://inabj.org/index.php/ibj/article/view/1800 |
| spellingShingle | Erlina Sih Mahanani Ikhsan Nur Arifin Arya Nur Ihsan Yusrina Lukitasari Ferry Sandra Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer Indonesian Biomedical Journal |
| title | Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer |
| title_full | Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer |
| title_fullStr | Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer |
| title_full_unstemmed | Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer |
| title_short | Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer |
| title_sort | parkia speciosa seeds ethanol extract as co chemotherapeutic agent for doxorubicin toward tongue cancer |
| url | https://inabj.org/index.php/ibj/article/view/1800 |
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