16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis.
We aim to evaluate the accuracy of the 16S ribosomal ribonucleic acid (rRNA) gene polymerase chain reaction (PCR) test in the diagnosis of bloodstream infections through a systematic review and meta-analysis.A computerized literature search was conducted to identify studies that assessed the diagnos...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4440735?pdf=render |
| _version_ | 1828386157269876736 |
|---|---|
| author | Guoming Su Zhuqing Fu Liren Hu Yueying Wang Zuguo Zhao Weiqing Yang |
| author_facet | Guoming Su Zhuqing Fu Liren Hu Yueying Wang Zuguo Zhao Weiqing Yang |
| author_sort | Guoming Su |
| collection | DOAJ |
| description | We aim to evaluate the accuracy of the 16S ribosomal ribonucleic acid (rRNA) gene polymerase chain reaction (PCR) test in the diagnosis of bloodstream infections through a systematic review and meta-analysis.A computerized literature search was conducted to identify studies that assessed the diagnostic value of 16S rRNA gene PCR test for bloodstream infections. Study quality was assessed using the revised Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. We calculated the sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and their 95% confidence intervals (95% CI) for each study. Summary receiver operating characteristic (SROC) curve was used to summarize overall test performance. Statistical analysis was performed in Meta-DiSc 1.4 and Stata/SE 12.0 software.Twenty-eight studies were included in our meta-analysis. Using random-effect model analysis, the pooled sensitivity, specificity, PLR, NLR, and DOR were 0.87 (95% CI, 0.85-0.89), 0.94 (95% CI, 0.93-0.95), 12.65 (95% CI, 8.04-19.90), 0.14 (95% CI, 0.08-0.24), and 116.76 (95% CI, 52.02-262.05), respectively. The SROC curve indicated that the area under the curve (AUC) was 0.9690 and the maximum joint sensitivity and specificity (Q*) was 0.9183. In addition, heterogeneity was statistically significant but was not caused by the threshold effect.Existing data suggest that 16S rRNA gene PCR test is a practical tool for the rapid screening of sepsis. Further prospective studies are needed to assess the diagnostic value of PCR amplification and DNA microarray hybridization of 16S rRNA gene in the future. |
| first_indexed | 2024-12-10T05:32:22Z |
| format | Article |
| id | doaj.art-f53a5aa6e0364d59829b10b7c54099d1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:32:22Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f53a5aa6e0364d59829b10b7c54099d12022-12-22T02:00:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012719510.1371/journal.pone.012719516S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis.Guoming SuZhuqing FuLiren HuYueying WangZuguo ZhaoWeiqing YangWe aim to evaluate the accuracy of the 16S ribosomal ribonucleic acid (rRNA) gene polymerase chain reaction (PCR) test in the diagnosis of bloodstream infections through a systematic review and meta-analysis.A computerized literature search was conducted to identify studies that assessed the diagnostic value of 16S rRNA gene PCR test for bloodstream infections. Study quality was assessed using the revised Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. We calculated the sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and their 95% confidence intervals (95% CI) for each study. Summary receiver operating characteristic (SROC) curve was used to summarize overall test performance. Statistical analysis was performed in Meta-DiSc 1.4 and Stata/SE 12.0 software.Twenty-eight studies were included in our meta-analysis. Using random-effect model analysis, the pooled sensitivity, specificity, PLR, NLR, and DOR were 0.87 (95% CI, 0.85-0.89), 0.94 (95% CI, 0.93-0.95), 12.65 (95% CI, 8.04-19.90), 0.14 (95% CI, 0.08-0.24), and 116.76 (95% CI, 52.02-262.05), respectively. The SROC curve indicated that the area under the curve (AUC) was 0.9690 and the maximum joint sensitivity and specificity (Q*) was 0.9183. In addition, heterogeneity was statistically significant but was not caused by the threshold effect.Existing data suggest that 16S rRNA gene PCR test is a practical tool for the rapid screening of sepsis. Further prospective studies are needed to assess the diagnostic value of PCR amplification and DNA microarray hybridization of 16S rRNA gene in the future.http://europepmc.org/articles/PMC4440735?pdf=render |
| spellingShingle | Guoming Su Zhuqing Fu Liren Hu Yueying Wang Zuguo Zhao Weiqing Yang 16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis. PLoS ONE |
| title | 16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis. |
| title_full | 16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis. |
| title_fullStr | 16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis. |
| title_full_unstemmed | 16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis. |
| title_short | 16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis. |
| title_sort | 16s ribosomal ribonucleic acid gene polymerase chain reaction in the diagnosis of bloodstream infections a systematic review and meta analysis |
| url | http://europepmc.org/articles/PMC4440735?pdf=render |
| work_keys_str_mv | AT guomingsu 16sribosomalribonucleicacidgenepolymerasechainreactioninthediagnosisofbloodstreaminfectionsasystematicreviewandmetaanalysis AT zhuqingfu 16sribosomalribonucleicacidgenepolymerasechainreactioninthediagnosisofbloodstreaminfectionsasystematicreviewandmetaanalysis AT lirenhu 16sribosomalribonucleicacidgenepolymerasechainreactioninthediagnosisofbloodstreaminfectionsasystematicreviewandmetaanalysis AT yueyingwang 16sribosomalribonucleicacidgenepolymerasechainreactioninthediagnosisofbloodstreaminfectionsasystematicreviewandmetaanalysis AT zuguozhao 16sribosomalribonucleicacidgenepolymerasechainreactioninthediagnosisofbloodstreaminfectionsasystematicreviewandmetaanalysis AT weiqingyang 16sribosomalribonucleicacidgenepolymerasechainreactioninthediagnosisofbloodstreaminfectionsasystematicreviewandmetaanalysis |