Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology tests
BackgroundHuman bocavirus 1 (HBoV1), first discovered in 2005, was positive in symptomatic and healthy children and co-detected with other respiratory viruses. It is a long journey to decisively demonstrate the unique viral pathogenic function of acute respiratory tract infection (ARTI) in pediatric...
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.932858/full |
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| author | Ri De Ri De Ke-Xiang Zhang Ke-Xiang Zhang Fang Wang Yu-Tong Zhou Yu Sun Dong-Mei Chen Ru-Nan Zhu Qi Guo Shuang Liu Dong Qu Yuan Qian Lin-Qing Zhao Lin-Qing Zhao |
| author_facet | Ri De Ri De Ke-Xiang Zhang Ke-Xiang Zhang Fang Wang Yu-Tong Zhou Yu Sun Dong-Mei Chen Ru-Nan Zhu Qi Guo Shuang Liu Dong Qu Yuan Qian Lin-Qing Zhao Lin-Qing Zhao |
| author_sort | Ri De |
| collection | DOAJ |
| description | BackgroundHuman bocavirus 1 (HBoV1), first discovered in 2005, was positive in symptomatic and healthy children and co-detected with other respiratory viruses. It is a long journey to decisively demonstrate the unique viral pathogenic function of acute respiratory tract infection (ARTI) in pediatric patients.MethodsRespiratory specimens collected from pediatric patients with ARTI from January 2017 to December 2021 were screened by a capillary electrophoresis-based multiplex PCR (CEMP) assay, then genotyped by PCR and sequencing for HBoV1. For the antigen test, a part of HBoV1 DNA positive nasopharyngeal aspirates (NPAs) was used as an antigen, while a rabbit anti-HBoV1 DR2 specific to HBoV1 was used as an antibody in the indirect-immunofluorescence assay (IFA). Finally, the levels of IgG specific to HBoV1 in acute and convalescent sera selected retrospectively from only HBoV1 DNA-positive patients were evaluated by IFA.ResultsAmong 9,899 specimens, 681 were positive for HBoV1 DNA (6.88%, 681/9899), which included 336 positives only for HBoV1 (49.34%, 336/681) and 345 (50.66%, 345/681) positives also for other pathogens. In the antigen test, there were 37 among 47 NPAs determined as HBoV1 antigen-positive (78.72%, 37/47), including 18 (48.65%, 18/37) positives solely for HBoV1 DNA. Among 4 pediatric patients with both acute and convalescent sera, there was one positive for HBoV1 antigen (D8873) and 2 lack the antigen results (D1474 and D10792), which showed seroconversion with a ≥ 4-fold increase in IgG levels.ConclusionsThe combination results of nucleic acid, antigen, and serology tests answered that HBoV1 is a genuine pathogen for ARTI in pediatric patients. |
| first_indexed | 2024-04-12T08:29:12Z |
| format | Article |
| id | doaj.art-f541b08d374a48cfa97c4f0d7f178b46 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T08:29:12Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-f541b08d374a48cfa97c4f0d7f178b462022-12-22T03:40:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.932858932858Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology testsRi De0Ri De1Ke-Xiang Zhang2Ke-Xiang Zhang3Fang Wang4Yu-Tong Zhou5Yu Sun6Dong-Mei Chen7Ru-Nan Zhu8Qi Guo9Shuang Liu10Dong Qu11Yuan Qian12Lin-Qing Zhao13Lin-Qing Zhao14Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaGraduate School of Peking Union Medical College, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaGraduate School of Peking Union Medical College, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Intensive Care Unit, Affiliated Children's Hospital, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Intensive Care Unit, Affiliated Children's Hospital, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaGraduate School of Peking Union Medical College, Beijing, ChinaBackgroundHuman bocavirus 1 (HBoV1), first discovered in 2005, was positive in symptomatic and healthy children and co-detected with other respiratory viruses. It is a long journey to decisively demonstrate the unique viral pathogenic function of acute respiratory tract infection (ARTI) in pediatric patients.MethodsRespiratory specimens collected from pediatric patients with ARTI from January 2017 to December 2021 were screened by a capillary electrophoresis-based multiplex PCR (CEMP) assay, then genotyped by PCR and sequencing for HBoV1. For the antigen test, a part of HBoV1 DNA positive nasopharyngeal aspirates (NPAs) was used as an antigen, while a rabbit anti-HBoV1 DR2 specific to HBoV1 was used as an antibody in the indirect-immunofluorescence assay (IFA). Finally, the levels of IgG specific to HBoV1 in acute and convalescent sera selected retrospectively from only HBoV1 DNA-positive patients were evaluated by IFA.ResultsAmong 9,899 specimens, 681 were positive for HBoV1 DNA (6.88%, 681/9899), which included 336 positives only for HBoV1 (49.34%, 336/681) and 345 (50.66%, 345/681) positives also for other pathogens. In the antigen test, there were 37 among 47 NPAs determined as HBoV1 antigen-positive (78.72%, 37/47), including 18 (48.65%, 18/37) positives solely for HBoV1 DNA. Among 4 pediatric patients with both acute and convalescent sera, there was one positive for HBoV1 antigen (D8873) and 2 lack the antigen results (D1474 and D10792), which showed seroconversion with a ≥ 4-fold increase in IgG levels.ConclusionsThe combination results of nucleic acid, antigen, and serology tests answered that HBoV1 is a genuine pathogen for ARTI in pediatric patients.https://www.frontiersin.org/articles/10.3389/fmicb.2022.932858/fullhuman bocavirus 1pediatric patientspathogenic roleacute respiratory tract infection (ARTI)nasopharyngeal aspirates |
| spellingShingle | Ri De Ri De Ke-Xiang Zhang Ke-Xiang Zhang Fang Wang Yu-Tong Zhou Yu Sun Dong-Mei Chen Ru-Nan Zhu Qi Guo Shuang Liu Dong Qu Yuan Qian Lin-Qing Zhao Lin-Qing Zhao Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology tests Frontiers in Microbiology human bocavirus 1 pediatric patients pathogenic role acute respiratory tract infection (ARTI) nasopharyngeal aspirates |
| title | Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology tests |
| title_full | Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology tests |
| title_fullStr | Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology tests |
| title_full_unstemmed | Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology tests |
| title_short | Human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid, antigen, and serology tests |
| title_sort | human bocavirus 1 is a genuine pathogen for acute respiratory tract infection in pediatric patients determined by nucleic acid antigen and serology tests |
| topic | human bocavirus 1 pediatric patients pathogenic role acute respiratory tract infection (ARTI) nasopharyngeal aspirates |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.932858/full |
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