Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients
Senile osteoporosis (SOP) is a worldwide age-related disease characterized by the loss of bone mass and decrease in bone strength. Bone mesenchymal stem cells (BMSCs) play an important role in the pathology of senile osteoporosis. Abnormal expression and regulation of non-coding RNA (ncRNA) are invo...
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| Format: | Article |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.776984/full |
| _version_ | 1819095758937260032 |
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| author | Yiyun Geng Yiyun Geng Jinfu Chen Chongfei Chang Yifen Zhang Li Duan Li Duan Weimin Zhu Lisha Mou Jianyi Xiong Jianyi Xiong Daping Wang Daping Wang |
| author_facet | Yiyun Geng Yiyun Geng Jinfu Chen Chongfei Chang Yifen Zhang Li Duan Li Duan Weimin Zhu Lisha Mou Jianyi Xiong Jianyi Xiong Daping Wang Daping Wang |
| author_sort | Yiyun Geng |
| collection | DOAJ |
| description | Senile osteoporosis (SOP) is a worldwide age-related disease characterized by the loss of bone mass and decrease in bone strength. Bone mesenchymal stem cells (BMSCs) play an important role in the pathology of senile osteoporosis. Abnormal expression and regulation of non-coding RNA (ncRNA) are involved in a variety of human diseases. In the present study, we aimed to identify differentially expressed mRNAs and ncRNAs in senile osteoporosis patient-derived BMSCs via high-throughput transcriptome sequencing in combination with bioinformatics analysis. As a result, 415 mRNAs, 30 lncRNAs, 6 circRNAs and 27 miRNAs were found to be significantly changed in the senile osteoporosis group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were applied to analyze the function of differentially expressed mRNAs and ncRNAs. The circRNA–miRNA–mRNA regulatory network was constructed using the cytoHubba plugin based on the Cytoscape software. Interestingly, circRNA008876-miR-150-5p-mRNA was the sole predicted circRNA-miRNA-mRNA network. The differential expression profile of this ceRNA network was further verified by qRT-PCR. The biological function of this network was validated by overexpression and knockdown experiments. In conclusion, circRNA008876-miR-150-5p-mRNA could be an important ceRNA network involved in senile osteoporosis, which provides potential biomarkers and therapeutic targets for senile osteoporosis. |
| first_indexed | 2024-12-21T23:48:24Z |
| format | Article |
| id | doaj.art-f54285e9eb694f05b7046f9ad43f7316 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-21T23:48:24Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-f54285e9eb694f05b7046f9ad43f73162022-12-21T18:46:00ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-12-011210.3389/fgene.2021.776984776984Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis PatientsYiyun Geng0Yiyun Geng1Jinfu Chen2Chongfei Chang3Yifen Zhang4Li Duan5Li Duan6Weimin Zhu7Lisha Mou8Jianyi Xiong9Jianyi Xiong10Daping Wang11Daping Wang12Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaSchool of Biotechnology and Food Engineering, Changshu Institute of Technology, Suzhou, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen, ChinaShenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, ChinaGuangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen, ChinaSenile osteoporosis (SOP) is a worldwide age-related disease characterized by the loss of bone mass and decrease in bone strength. Bone mesenchymal stem cells (BMSCs) play an important role in the pathology of senile osteoporosis. Abnormal expression and regulation of non-coding RNA (ncRNA) are involved in a variety of human diseases. In the present study, we aimed to identify differentially expressed mRNAs and ncRNAs in senile osteoporosis patient-derived BMSCs via high-throughput transcriptome sequencing in combination with bioinformatics analysis. As a result, 415 mRNAs, 30 lncRNAs, 6 circRNAs and 27 miRNAs were found to be significantly changed in the senile osteoporosis group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were applied to analyze the function of differentially expressed mRNAs and ncRNAs. The circRNA–miRNA–mRNA regulatory network was constructed using the cytoHubba plugin based on the Cytoscape software. Interestingly, circRNA008876-miR-150-5p-mRNA was the sole predicted circRNA-miRNA-mRNA network. The differential expression profile of this ceRNA network was further verified by qRT-PCR. The biological function of this network was validated by overexpression and knockdown experiments. In conclusion, circRNA008876-miR-150-5p-mRNA could be an important ceRNA network involved in senile osteoporosis, which provides potential biomarkers and therapeutic targets for senile osteoporosis.https://www.frontiersin.org/articles/10.3389/fgene.2021.776984/fullsenile osteoporosishuman bone marrow mesenchymal stem cellswhole transcriptome sequencingnon-coding RNAceRNA network |
| spellingShingle | Yiyun Geng Yiyun Geng Jinfu Chen Chongfei Chang Yifen Zhang Li Duan Li Duan Weimin Zhu Lisha Mou Jianyi Xiong Jianyi Xiong Daping Wang Daping Wang Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients Frontiers in Genetics senile osteoporosis human bone marrow mesenchymal stem cells whole transcriptome sequencing non-coding RNA ceRNA network |
| title | Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients |
| title_full | Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients |
| title_fullStr | Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients |
| title_full_unstemmed | Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients |
| title_short | Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients |
| title_sort | systematic analysis of mrnas and ncrnas in bmscs of senile osteoporosis patients |
| topic | senile osteoporosis human bone marrow mesenchymal stem cells whole transcriptome sequencing non-coding RNA ceRNA network |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.776984/full |
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