Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populations
Abstract Background Heterosis has been widely used in maize breeding. However, we know little about the heterotic quantitative trait loci and their roles in genomic prediction. In this study, we sought to identify heterotic quantitative trait loci for seedling biomass-related traits using triple tes...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13007-021-00785-8 |
| _version_ | 1830483638824730624 |
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| author | Tifu Zhang Lu Jiang Long Ruan Yiliang Qian Shuaiqiang Liang Feng Lin Haiyan Lu Huixue Dai Han Zhao |
| author_facet | Tifu Zhang Lu Jiang Long Ruan Yiliang Qian Shuaiqiang Liang Feng Lin Haiyan Lu Huixue Dai Han Zhao |
| author_sort | Tifu Zhang |
| collection | DOAJ |
| description | Abstract Background Heterosis has been widely used in maize breeding. However, we know little about the heterotic quantitative trait loci and their roles in genomic prediction. In this study, we sought to identify heterotic quantitative trait loci for seedling biomass-related traits using triple testcross design and compare their prediction accuracies by fitting molecular markers and heterotic quantitative trait loci. Results A triple testcross population comprised of 366 genotypes was constructed by crossing each of 122 intermated B73 × Mo17 genotypes with B73, Mo17, and B73 × Mo17. The mid-parent heterosis of seedling biomass-related traits involved in leaf length, leaf width, leaf area, and seedling dry weight displayed a large range, from less than 50 to ~ 150%. Relationships between heterosis of seedling biomass-related traits showed congruency with that between performances. Based on a linkage map comprised of 1631 markers, 14 augmented additive, two augmented dominance, and three dominance × additive epistatic quantitative trait loci for heterosis of seedling biomass-related traits were identified, with each individually explaining 4.1–20.5% of the phenotypic variation. All modes of gene action, i.e., additive, partially dominant, dominant, and overdominant modes were observed. In addition, ten additive × additive and six dominance × dominance epistatic interactions were identified. By implementing the general and special combining ability model, we found that prediction accuracy ranged from 0.29 for leaf length to 0.56 for leaf width. Different number of marker analysis showed that ~ 800 markers almost capture the largest prediction accuracies. When incorporating the heterotic quantitative trait loci into the model, we did not find the significant change of prediction accuracy, with only leaf length showing the marginal improvement by 1.7%. Conclusions Our results demonstrated that the triple testcross design is suitable for detecting heterotic quantitative trait loci and evaluating the prediction accuracy. Seedling leaf width can be used as the representative trait for seedling prediction. The heterotic quantitative trait loci are not necessary for genomic prediction of seedling biomass-related traits. |
| first_indexed | 2024-12-21T17:54:42Z |
| format | Article |
| id | doaj.art-f542baffc5464881aaeee5f5babafb68 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-12-21T17:54:42Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-f542baffc5464881aaeee5f5babafb682022-12-21T18:55:14ZengBMCPlant Methods1746-48112021-07-0117111110.1186/s13007-021-00785-8Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populationsTifu Zhang0Lu Jiang1Long Ruan2Yiliang Qian3Shuaiqiang Liang4Feng Lin5Haiyan Lu6Huixue Dai7Han Zhao8Jiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural SciencesJiangsu Provincial Key Laboratory of Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural SciencesInstitute of Tobacco, Anhui Academy of Agricultural SciencesInstitute of Tobacco, Anhui Academy of Agricultural SciencesJiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural SciencesJiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural SciencesJiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural SciencesNanjing Institute of Vegetable SciencesJiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural SciencesAbstract Background Heterosis has been widely used in maize breeding. However, we know little about the heterotic quantitative trait loci and their roles in genomic prediction. In this study, we sought to identify heterotic quantitative trait loci for seedling biomass-related traits using triple testcross design and compare their prediction accuracies by fitting molecular markers and heterotic quantitative trait loci. Results A triple testcross population comprised of 366 genotypes was constructed by crossing each of 122 intermated B73 × Mo17 genotypes with B73, Mo17, and B73 × Mo17. The mid-parent heterosis of seedling biomass-related traits involved in leaf length, leaf width, leaf area, and seedling dry weight displayed a large range, from less than 50 to ~ 150%. Relationships between heterosis of seedling biomass-related traits showed congruency with that between performances. Based on a linkage map comprised of 1631 markers, 14 augmented additive, two augmented dominance, and three dominance × additive epistatic quantitative trait loci for heterosis of seedling biomass-related traits were identified, with each individually explaining 4.1–20.5% of the phenotypic variation. All modes of gene action, i.e., additive, partially dominant, dominant, and overdominant modes were observed. In addition, ten additive × additive and six dominance × dominance epistatic interactions were identified. By implementing the general and special combining ability model, we found that prediction accuracy ranged from 0.29 for leaf length to 0.56 for leaf width. Different number of marker analysis showed that ~ 800 markers almost capture the largest prediction accuracies. When incorporating the heterotic quantitative trait loci into the model, we did not find the significant change of prediction accuracy, with only leaf length showing the marginal improvement by 1.7%. Conclusions Our results demonstrated that the triple testcross design is suitable for detecting heterotic quantitative trait loci and evaluating the prediction accuracy. Seedling leaf width can be used as the representative trait for seedling prediction. The heterotic quantitative trait loci are not necessary for genomic prediction of seedling biomass-related traits.https://doi.org/10.1186/s13007-021-00785-8Seedling biomass-related traitsHeterotic quantitative trait lociGenomic predictionTriple testcrossMaize |
| spellingShingle | Tifu Zhang Lu Jiang Long Ruan Yiliang Qian Shuaiqiang Liang Feng Lin Haiyan Lu Huixue Dai Han Zhao Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populations Plant Methods Seedling biomass-related traits Heterotic quantitative trait loci Genomic prediction Triple testcross Maize |
| title | Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populations |
| title_full | Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populations |
| title_fullStr | Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populations |
| title_full_unstemmed | Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populations |
| title_short | Heterotic quantitative trait loci analysis and genomic prediction of seedling biomass-related traits in maize triple testcross populations |
| title_sort | heterotic quantitative trait loci analysis and genomic prediction of seedling biomass related traits in maize triple testcross populations |
| topic | Seedling biomass-related traits Heterotic quantitative trait loci Genomic prediction Triple testcross Maize |
| url | https://doi.org/10.1186/s13007-021-00785-8 |
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