A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette Smokers
Tobacco consumption alters many biological parameters, including α-amylase activity. This study reports the effect of cigarette smoking on salivary α-amylase activities of some male heavy smokers (100) in apparent good health compared with (40) male non-smokers as control. The salivary α-amylase ac...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Omar Al-Mukhtar University
2020-09-01
|
| Series: | مجلة المختار للعلوم |
| Subjects: | |
| Online Access: | https://omu.edu.ly/journals/index.php/mjsc/article/view/295 |
| _version_ | 1797713319049560064 |
|---|---|
| author | Khaled S. Al salhen Omalsaad E. I. Omar Latefa A. Aljradmi |
| author_facet | Khaled S. Al salhen Omalsaad E. I. Omar Latefa A. Aljradmi |
| author_sort | Khaled S. Al salhen |
| collection | DOAJ |
| description |
Tobacco consumption alters many biological parameters, including α-amylase activity. This study reports the effect of cigarette smoking on salivary α-amylase activities of some male heavy smokers (100) in apparent good health compared with (40) male non-smokers as control. The salivary α-amylase activity was assayed using the standard colorimetric method. The α-amylase enzyme was characterized from the saliva samples collected from healthy adult smokers and non-smokers (control) to determine the effects of temperature, pH, and substrate concentration on the kinetic parameters of the enzyme. The analysis of the saliva samples showed that there were significant increases (P<0.05) in the α-amylase activity values in the saliva of cigarette smokers when compared with non-smokers at optimum conditions (2.74±0.37 nmol/min/mg-protein and 2.01±0.16 nmol/min/mg-protein, respectively). The Michaelis constant (Km) values of 0.5±0.02 mM and 1.12±0.08 mM observed for non-smokers and smokers were obtained from the double reciprocal plot of initial velocity (1/V) and substrate concentration (1/[S]). The maximum activities obtained at an optimum temperature of 37ºC, and an apparent pH of 7.0 for both subjects were 31.25±8.24 nmol/min/mg protein and 18.10±4.06 nmol/min/mg protein for non-smokers and smokers respectively. Measurements of salivary α-amylase activity could be useful in the early detection and diagnosis of parotitis. Subsequent studies are required to specify the mechanisms responsible for such a risk.
|
| first_indexed | 2024-03-12T07:34:39Z |
| format | Article |
| id | doaj.art-f545ec6b42744184b726311f028cb3bf |
| institution | Directory Open Access Journal |
| issn | 2617-2178 2617-2186 |
| language | English |
| last_indexed | 2024-03-12T07:34:39Z |
| publishDate | 2020-09-01 |
| publisher | Omar Al-Mukhtar University |
| record_format | Article |
| series | مجلة المختار للعلوم |
| spelling | doaj.art-f545ec6b42744184b726311f028cb3bf2023-09-02T21:36:26ZengOmar Al-Mukhtar Universityمجلة المختار للعلوم2617-21782617-21862020-09-0135310.54172/mjsc.v35i3.295A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette SmokersKhaled S. Al salhen0Omalsaad E. I. Omar1Latefa A. Aljradmi2Departmentof Chemistry Faculty of Science, Omar Al-Mukhater University, El-Beida, LibyaBiochemistry Department, Faculty of Medicine, El-Beida, LibyaDepartmentof Chemistry Faculty of Science, Omar Al-Mukhater University, El-Beida, Libya Tobacco consumption alters many biological parameters, including α-amylase activity. This study reports the effect of cigarette smoking on salivary α-amylase activities of some male heavy smokers (100) in apparent good health compared with (40) male non-smokers as control. The salivary α-amylase activity was assayed using the standard colorimetric method. The α-amylase enzyme was characterized from the saliva samples collected from healthy adult smokers and non-smokers (control) to determine the effects of temperature, pH, and substrate concentration on the kinetic parameters of the enzyme. The analysis of the saliva samples showed that there were significant increases (P<0.05) in the α-amylase activity values in the saliva of cigarette smokers when compared with non-smokers at optimum conditions (2.74±0.37 nmol/min/mg-protein and 2.01±0.16 nmol/min/mg-protein, respectively). The Michaelis constant (Km) values of 0.5±0.02 mM and 1.12±0.08 mM observed for non-smokers and smokers were obtained from the double reciprocal plot of initial velocity (1/V) and substrate concentration (1/[S]). The maximum activities obtained at an optimum temperature of 37ºC, and an apparent pH of 7.0 for both subjects were 31.25±8.24 nmol/min/mg protein and 18.10±4.06 nmol/min/mg protein for non-smokers and smokers respectively. Measurements of salivary α-amylase activity could be useful in the early detection and diagnosis of parotitis. Subsequent studies are required to specify the mechanisms responsible for such a risk. https://omu.edu.ly/journals/index.php/mjsc/article/view/295Cigarette SmokersSalivary α-AmylaseKinetic ParametersOptimum Conditions |
| spellingShingle | Khaled S. Al salhen Omalsaad E. I. Omar Latefa A. Aljradmi A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette Smokers مجلة المختار للعلوم Cigarette Smokers Salivary α-Amylase Kinetic Parameters Optimum Conditions |
| title | A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette Smokers |
| title_full | A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette Smokers |
| title_fullStr | A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette Smokers |
| title_full_unstemmed | A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette Smokers |
| title_short | A Biochemical Study of α-Amylase Activity in Saliva of Some Libyan Cigarette Smokers |
| title_sort | biochemical study of α amylase activity in saliva of some libyan cigarette smokers |
| topic | Cigarette Smokers Salivary α-Amylase Kinetic Parameters Optimum Conditions |
| url | https://omu.edu.ly/journals/index.php/mjsc/article/view/295 |
| work_keys_str_mv | AT khaledsalsalhen abiochemicalstudyofaamylaseactivityinsalivaofsomelibyancigarettesmokers AT omalsaadeiomar abiochemicalstudyofaamylaseactivityinsalivaofsomelibyancigarettesmokers AT latefaaaljradmi abiochemicalstudyofaamylaseactivityinsalivaofsomelibyancigarettesmokers AT khaledsalsalhen biochemicalstudyofaamylaseactivityinsalivaofsomelibyancigarettesmokers AT omalsaadeiomar biochemicalstudyofaamylaseactivityinsalivaofsomelibyancigarettesmokers AT latefaaaljradmi biochemicalstudyofaamylaseactivityinsalivaofsomelibyancigarettesmokers |