Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study
(1) In recent years, there has been a significant increase in the availability of denture adhesives for stabilizing removable dentures. The aim of the present study was to assess the cytotoxicity of three denture adhesives on human fibroblasts. (2) Methods: Three denture adhesives were analyzed. Fib...
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| Online Access: | https://www.mdpi.com/1996-1944/15/4/1583 |
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| author | Ewa Sobolewska Piotr Makowiecki Justyna Drozdowska Ireneusz Dziuba Alicja Nowicka Marzena Wyganowska-Świątkowska Joanna Janiszewska-Olszowska Katarzyna Grocholewicz |
| author_facet | Ewa Sobolewska Piotr Makowiecki Justyna Drozdowska Ireneusz Dziuba Alicja Nowicka Marzena Wyganowska-Świątkowska Joanna Janiszewska-Olszowska Katarzyna Grocholewicz |
| author_sort | Ewa Sobolewska |
| collection | DOAJ |
| description | (1) In recent years, there has been a significant increase in the availability of denture adhesives for stabilizing removable dentures. The aim of the present study was to assess the cytotoxicity of three denture adhesives on human fibroblasts. (2) Methods: Three denture adhesives were analyzed. Fibroblast cultures were established for the study and control groups in order to assess the incidence of necrosis and to evaluate the microscopic intracellular alterations induced. Following incubation with (study groups) or without adhesives (control group), trypan blue dye exclusion assay was used to determine the number of viable and/or dead cells. Microscopic specimens were stained with haematoxylin and eosin, scanned, digitally processed and then analyzed by a histopathologist. (3) Results: All three denture adhesives analyzed demonstrated various toxic effects in vitro on human fibroblast: quantitative evaluation—45.87–61.13% reduction of cell viability (<i>p</i> = 0.0001) and slight to moderate cytotoxicity in qualitative evaluation. (4) Conclusions: Denture adhesive creams demonstrated a toxic effect on human fibroblasts in vitro in quantitative and qualitative evaluation. In vivo observations are needed to find out if denture adhesives present a cytotoxic effect in patients. |
| first_indexed | 2024-03-09T21:31:58Z |
| format | Article |
| id | doaj.art-f546050891af4c0985bebbb062614692 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T21:31:58Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-f546050891af4c0985bebbb0626146922023-11-23T20:55:36ZengMDPI AGMaterials1996-19442022-02-01154158310.3390/ma15041583Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro StudyEwa Sobolewska0Piotr Makowiecki1Justyna Drozdowska2Ireneusz Dziuba3Alicja Nowicka4Marzena Wyganowska-Świątkowska5Joanna Janiszewska-Olszowska6Katarzyna Grocholewicz7Department of Dental Prosthetics, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Radiology, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Interdisciplinary Dentistry, Pomeranian Medical University, 70-111 Szczecin, PolandFaculty of Medicine, University of Technology, 40-555 Katowice, PolandDepartment of Conservative Dentistry and Endodontics, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Oral Surgery and Periodontology, Poznan University of Medical Sciences, 60-812 Poznan, PolandDepartment of Interdisciplinary Dentistry, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Interdisciplinary Dentistry, Pomeranian Medical University, 70-111 Szczecin, Poland(1) In recent years, there has been a significant increase in the availability of denture adhesives for stabilizing removable dentures. The aim of the present study was to assess the cytotoxicity of three denture adhesives on human fibroblasts. (2) Methods: Three denture adhesives were analyzed. Fibroblast cultures were established for the study and control groups in order to assess the incidence of necrosis and to evaluate the microscopic intracellular alterations induced. Following incubation with (study groups) or without adhesives (control group), trypan blue dye exclusion assay was used to determine the number of viable and/or dead cells. Microscopic specimens were stained with haematoxylin and eosin, scanned, digitally processed and then analyzed by a histopathologist. (3) Results: All three denture adhesives analyzed demonstrated various toxic effects in vitro on human fibroblast: quantitative evaluation—45.87–61.13% reduction of cell viability (<i>p</i> = 0.0001) and slight to moderate cytotoxicity in qualitative evaluation. (4) Conclusions: Denture adhesive creams demonstrated a toxic effect on human fibroblasts in vitro in quantitative and qualitative evaluation. In vivo observations are needed to find out if denture adhesives present a cytotoxic effect in patients.https://www.mdpi.com/1996-1944/15/4/1583denturesdenture adhesiveshuman fibroblastscytotoxicity |
| spellingShingle | Ewa Sobolewska Piotr Makowiecki Justyna Drozdowska Ireneusz Dziuba Alicja Nowicka Marzena Wyganowska-Świątkowska Joanna Janiszewska-Olszowska Katarzyna Grocholewicz Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study Materials dentures denture adhesives human fibroblasts cytotoxicity |
| title | Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study |
| title_full | Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study |
| title_fullStr | Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study |
| title_full_unstemmed | Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study |
| title_short | Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study |
| title_sort | cytotoxic potential of denture adhesives on human fibroblasts in vitro study |
| topic | dentures denture adhesives human fibroblasts cytotoxicity |
| url | https://www.mdpi.com/1996-1944/15/4/1583 |
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