Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it>
<p>Summary</p> <p>Background</p> <p><it>Escherichia coli </it>induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the b...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-04-01
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| Series: | Microbial Cell Factories |
| Online Access: | http://www.microbialcellfactories.com/content/4/1/9 |
| _version_ | 1818806232959418368 |
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| author | Vasala Antti Panula Johanna Weinel Stefan Böhm Daniela Mayer Christina Falschlehner Christina Soini Jaakko Neubauer Peter |
| author_facet | Vasala Antti Panula Johanna Weinel Stefan Böhm Daniela Mayer Christina Falschlehner Christina Soini Jaakko Neubauer Peter |
| author_sort | Vasala Antti |
| collection | DOAJ |
| description | <p>Summary</p> <p>Background</p> <p><it>Escherichia coli </it>induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuous function of the cell. Whereas it has been observed in eukaryotic cells, that the ATP level immediately decreased after the temperature shift, no data are available for <it>E. coli </it>about the adenosine nucleotide levels during the narrow time range of minutes after a temperature up-shift.</p> <p>Results</p> <p>The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake. Also the mRNA level of typical heat shock genes increases within only one minute after the heat-shock.</p> <p>Conclusion</p> <p>The presented data prove the very fast response of <it>E. coli </it>to a heat-shock and that the initial response includes the increase of the ATP pool which is important to fulfil the need of the cell for new syntheses, as well as for the function of chaperones and proteases.</p> |
| first_indexed | 2024-12-18T19:06:30Z |
| format | Article |
| id | doaj.art-f546a5745f7f4a1a835bcb77cd21ad4d |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-18T19:06:30Z |
| publishDate | 2005-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-f546a5745f7f4a1a835bcb77cd21ad4d2022-12-21T20:56:24ZengBMCMicrobial Cell Factories1475-28592005-04-0141910.1186/1475-2859-4-9Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it>Vasala AnttiPanula JohannaWeinel StefanBöhm DanielaMayer ChristinaFalschlehner ChristinaSoini JaakkoNeubauer Peter<p>Summary</p> <p>Background</p> <p><it>Escherichia coli </it>induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuous function of the cell. Whereas it has been observed in eukaryotic cells, that the ATP level immediately decreased after the temperature shift, no data are available for <it>E. coli </it>about the adenosine nucleotide levels during the narrow time range of minutes after a temperature up-shift.</p> <p>Results</p> <p>The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake. Also the mRNA level of typical heat shock genes increases within only one minute after the heat-shock.</p> <p>Conclusion</p> <p>The presented data prove the very fast response of <it>E. coli </it>to a heat-shock and that the initial response includes the increase of the ATP pool which is important to fulfil the need of the cell for new syntheses, as well as for the function of chaperones and proteases.</p>http://www.microbialcellfactories.com/content/4/1/9 |
| spellingShingle | Vasala Antti Panula Johanna Weinel Stefan Böhm Daniela Mayer Christina Falschlehner Christina Soini Jaakko Neubauer Peter Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it> Microbial Cell Factories |
| title | Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it> |
| title_full | Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it> |
| title_fullStr | Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it> |
| title_full_unstemmed | Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it> |
| title_short | Transient increase of ATP as a response to temperature up-shift in <it>Escherichia coli</it> |
| title_sort | transient increase of atp as a response to temperature up shift in it escherichia coli it |
| url | http://www.microbialcellfactories.com/content/4/1/9 |
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