Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo
Deer tendon, a deer processing byproduct, is an excellent protein source for the preparation of peptides for improving osteoporosis by its high protein content and high nutritional value. The optimal process of collagen acid extraction was implemented and the results showed that the acid concentrati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/17/6275 |
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| author | Chaoting Wen Dan Wang Zhiyi Zhang Guoyan Liu Li Liang Xiaofang Liu Jixian Zhang Youdong Li Xin Xu |
| author_facet | Chaoting Wen Dan Wang Zhiyi Zhang Guoyan Liu Li Liang Xiaofang Liu Jixian Zhang Youdong Li Xin Xu |
| author_sort | Chaoting Wen |
| collection | DOAJ |
| description | Deer tendon, a deer processing byproduct, is an excellent protein source for the preparation of peptides for improving osteoporosis by its high protein content and high nutritional value. The optimal process of collagen acid extraction was implemented and the results showed that the acid concentration was 7%, the material–liquid ratio was 1:25, and the soaking time was 48 h. DTCHs could promote MC3T3-E1 cell proliferation and increase alkaline phosphatase activities in vitro. In addition, compared with the model group, the DTCHs treatment groups with an oral dosage of 350, 750, and 1500 mg/kg rat/day could significantly improve the shape, weight, bone mechanics, and alkaline phosphatase activities of tail-suspended mice. Bone microstructure and mineralization also recovered significantly in vivo. This result is expected to provide the structural and biological information for DTCHs-based functional foods. |
| first_indexed | 2024-03-10T23:17:59Z |
| format | Article |
| id | doaj.art-f547bf49716446028fe5535a04fc192d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T23:17:59Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f547bf49716446028fe5535a04fc192d2023-11-19T08:33:44ZengMDPI AGMolecules1420-30492023-08-012817627510.3390/molecules28176275Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In VivoChaoting Wen0Dan Wang1Zhiyi Zhang2Guoyan Liu3Li Liang4Xiaofang Liu5Jixian Zhang6Youdong Li7Xin Xu8College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaDeer tendon, a deer processing byproduct, is an excellent protein source for the preparation of peptides for improving osteoporosis by its high protein content and high nutritional value. The optimal process of collagen acid extraction was implemented and the results showed that the acid concentration was 7%, the material–liquid ratio was 1:25, and the soaking time was 48 h. DTCHs could promote MC3T3-E1 cell proliferation and increase alkaline phosphatase activities in vitro. In addition, compared with the model group, the DTCHs treatment groups with an oral dosage of 350, 750, and 1500 mg/kg rat/day could significantly improve the shape, weight, bone mechanics, and alkaline phosphatase activities of tail-suspended mice. Bone microstructure and mineralization also recovered significantly in vivo. This result is expected to provide the structural and biological information for DTCHs-based functional foods.https://www.mdpi.com/1420-3049/28/17/6275collagen protein hydrolysatesdegree of hydrolysisintervention osteoporosis activitiesMC3T3-E1mouse tail-suspension test |
| spellingShingle | Chaoting Wen Dan Wang Zhiyi Zhang Guoyan Liu Li Liang Xiaofang Liu Jixian Zhang Youdong Li Xin Xu Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo Molecules collagen protein hydrolysates degree of hydrolysis intervention osteoporosis activities MC3T3-E1 mouse tail-suspension test |
| title | Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo |
| title_full | Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo |
| title_fullStr | Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo |
| title_full_unstemmed | Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo |
| title_short | Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo |
| title_sort | intervention effects of deer tendon collagen hydrolysates on osteoporosis in vitro and in vivo |
| topic | collagen protein hydrolysates degree of hydrolysis intervention osteoporosis activities MC3T3-E1 mouse tail-suspension test |
| url | https://www.mdpi.com/1420-3049/28/17/6275 |
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