ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells
Abstract Long non-coding RNAs (lncRNAs) play important roles in many pathophysiological processes, including cancer progression. Namely, lncRNA Receptor-tyrosine-kinase-like orphan receptor-1 antisense 1 (ROR1-AS1) is crucial for cancer occurrence and progression in organs such as the liver or bladd...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-023-11412-1 |
| _version_ | 1797559147717197824 |
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| author | Xueliang Li Zhaowei Sun Li Wang Qinlei Wang Maobing Wang Jingyun Guo Haoran Li MenShou Chen Guanghua Cao Yanan Yu Haochen Zhong Hao Zou Kai Ma Bingyuan Zhang Guolei Wang Yujie Feng |
| author_facet | Xueliang Li Zhaowei Sun Li Wang Qinlei Wang Maobing Wang Jingyun Guo Haoran Li MenShou Chen Guanghua Cao Yanan Yu Haochen Zhong Hao Zou Kai Ma Bingyuan Zhang Guolei Wang Yujie Feng |
| author_sort | Xueliang Li |
| collection | DOAJ |
| description | Abstract Long non-coding RNAs (lncRNAs) play important roles in many pathophysiological processes, including cancer progression. Namely, lncRNA Receptor-tyrosine-kinase-like orphan receptor-1 antisense 1 (ROR1-AS1) is crucial for cancer occurrence and progression in organs such as the liver or bladder. However, its expression and role in cholangiocarcinoma (CCA) have not been thoroughly explored. Firstly, we assessed cell viability, proliferation, invasion, and migration using three cell lines (HuCCT-1, QBC399, and RBE) to explore the biological characteristics of ROR1-AS1 in CCA. Secondly, to determine the in vivo effect of ROR1-AS1 on tumor growth, ROR1-AS1 knockdown (KD) HuCCT-1 cells were subcutaneously injected into nude mice to evaluate tumor growth. Finally, we conducted a bioinformatic analysis to confirm the role of ROR1-AS1 in the prognosis and immunity of CCA. In this study, we found that lncRNA ROR1-AS1 was increased in CCA samples and patients with higher ROR1-AS1 expression had a shorter overall survival period. siRNA-mediated KD of ROR1-AS1 significantly reduced cell proliferation and inhibited the migration of CCA cells. In addition, ROR1-AS1 KD HuCCT-1 cells injected into nude mice grew slower than normal CCA cells. In summary, our results show that ROR1-AS1 can promote CCA progression and might serve as a new target for diagnosis and treatment of CCA. |
| first_indexed | 2024-03-10T17:41:20Z |
| format | Article |
| id | doaj.art-f547cd5583564412bc18ba29d717b805 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-10T17:41:20Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-f547cd5583564412bc18ba29d717b8052023-11-20T09:43:10ZengBMCBMC Cancer1471-24072023-09-0123111210.1186/s12885-023-11412-1ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cellsXueliang Li0Zhaowei Sun1Li Wang2Qinlei Wang3Maobing Wang4Jingyun Guo5Haoran Li6MenShou Chen7Guanghua Cao8Yanan Yu9Haochen Zhong10Hao Zou11Kai Ma12Bingyuan Zhang13Guolei Wang14Yujie Feng15Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of operating theater, the Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, HuiKang Hospital of the Affiliated Hospital of Qingdao University of QingdaoDepartment of Internal Medicine-Cardiovascular, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, HuiKang Hospital of the Affiliated Hospital of Qingdao University of QingdaoDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityAbstract Long non-coding RNAs (lncRNAs) play important roles in many pathophysiological processes, including cancer progression. Namely, lncRNA Receptor-tyrosine-kinase-like orphan receptor-1 antisense 1 (ROR1-AS1) is crucial for cancer occurrence and progression in organs such as the liver or bladder. However, its expression and role in cholangiocarcinoma (CCA) have not been thoroughly explored. Firstly, we assessed cell viability, proliferation, invasion, and migration using three cell lines (HuCCT-1, QBC399, and RBE) to explore the biological characteristics of ROR1-AS1 in CCA. Secondly, to determine the in vivo effect of ROR1-AS1 on tumor growth, ROR1-AS1 knockdown (KD) HuCCT-1 cells were subcutaneously injected into nude mice to evaluate tumor growth. Finally, we conducted a bioinformatic analysis to confirm the role of ROR1-AS1 in the prognosis and immunity of CCA. In this study, we found that lncRNA ROR1-AS1 was increased in CCA samples and patients with higher ROR1-AS1 expression had a shorter overall survival period. siRNA-mediated KD of ROR1-AS1 significantly reduced cell proliferation and inhibited the migration of CCA cells. In addition, ROR1-AS1 KD HuCCT-1 cells injected into nude mice grew slower than normal CCA cells. In summary, our results show that ROR1-AS1 can promote CCA progression and might serve as a new target for diagnosis and treatment of CCA.https://doi.org/10.1186/s12885-023-11412-1CholangiocarcinomaImmune infiltrationEnrichment analysisPrognosisTumor proliferationMigration and invasion |
| spellingShingle | Xueliang Li Zhaowei Sun Li Wang Qinlei Wang Maobing Wang Jingyun Guo Haoran Li MenShou Chen Guanghua Cao Yanan Yu Haochen Zhong Hao Zou Kai Ma Bingyuan Zhang Guolei Wang Yujie Feng ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells BMC Cancer Cholangiocarcinoma Immune infiltration Enrichment analysis Prognosis Tumor proliferation Migration and invasion |
| title | ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells |
| title_full | ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells |
| title_fullStr | ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells |
| title_full_unstemmed | ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells |
| title_short | ROR1-AS1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells |
| title_sort | ror1 as1 might promote in vivo and in vitro proliferation and invasion of cholangiocarcinoma cells |
| topic | Cholangiocarcinoma Immune infiltration Enrichment analysis Prognosis Tumor proliferation Migration and invasion |
| url | https://doi.org/10.1186/s12885-023-11412-1 |
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