Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous mats
Developing novel, lightweight and biocompatible mesh for pelvic organ prolapse, a gynecological disease, has attracted the global interest of biomedical researchers. In our study, polycaprolactone and nylon 6 mats with excellent mechanical properties were fabricated via co-electrospinning. Positivel...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127521002690 |
| _version_ | 1818437412849713152 |
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| author | Fangfang Dai Jia Yu Mengqin Yuan Zhimin Deng Yanqing Wang Yaqi Fan Hongbing Deng Yanxiang Cheng |
| author_facet | Fangfang Dai Jia Yu Mengqin Yuan Zhimin Deng Yanqing Wang Yaqi Fan Hongbing Deng Yanxiang Cheng |
| author_sort | Fangfang Dai |
| collection | DOAJ |
| description | Developing novel, lightweight and biocompatible mesh for pelvic organ prolapse, a gynecological disease, has attracted the global interest of biomedical researchers. In our study, polycaprolactone and nylon 6 mats with excellent mechanical properties were fabricated via co-electrospinning. Positively charged chitosan with antimicrobial properties and negatively charged collagen with good biocompatibility were then assembled on the nanofiber mats via layer by layer (LBL) technology. SEM and mechanical property tests showed that LBL deposition increased the nanofibers’ diameter and elastic modulus. To evaluate the successful deposition process, the zeta potential, The Fourier-transform infrared, X-ray diffraction, and X-ray photoelectron spectroscopy analyses were conducted to systematically study the mats’ chemical characterization. The results indicated that the mats’ composition and structure significantly changed after LBL modification. Cell biology experiments demonstrated that the LBL-structured mats can facilitate cell growth, proliferation, and adhesion. Antibacterial tests confirmed their excellent antibacterial activity. Chitosan/Collagen-coated Polycaprolactone/Nylon 6 mats demonstrate considerable potential as implant materials for pelvic reestablishment in pelvic organ prolapse patients. |
| first_indexed | 2024-12-14T17:24:16Z |
| format | Article |
| id | doaj.art-f54a8bb176614f9cb34d32bc5ce66ffd |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-14T17:24:16Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-f54a8bb176614f9cb34d32bc5ce66ffd2022-12-21T22:53:16ZengElsevierMaterials & Design0264-12752021-07-01205109717Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous matsFangfang Dai0Jia Yu1Mengqin Yuan2Zhimin Deng3Yanqing Wang4Yaqi Fan5Hongbing Deng6Yanxiang Cheng7Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Engineering Center of Natural Polymers-based Medical Materials, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Hubei Key Laboratory of Digestive System Disease, Wuhan, Hubei 430060, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Engineering Center of Natural Polymers-based Medical Materials, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaShanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200050, ChinaHubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Engineering Center of Natural Polymers-based Medical Materials, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China; Corresponding authors.Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; Corresponding authors.Developing novel, lightweight and biocompatible mesh for pelvic organ prolapse, a gynecological disease, has attracted the global interest of biomedical researchers. In our study, polycaprolactone and nylon 6 mats with excellent mechanical properties were fabricated via co-electrospinning. Positively charged chitosan with antimicrobial properties and negatively charged collagen with good biocompatibility were then assembled on the nanofiber mats via layer by layer (LBL) technology. SEM and mechanical property tests showed that LBL deposition increased the nanofibers’ diameter and elastic modulus. To evaluate the successful deposition process, the zeta potential, The Fourier-transform infrared, X-ray diffraction, and X-ray photoelectron spectroscopy analyses were conducted to systematically study the mats’ chemical characterization. The results indicated that the mats’ composition and structure significantly changed after LBL modification. Cell biology experiments demonstrated that the LBL-structured mats can facilitate cell growth, proliferation, and adhesion. Antibacterial tests confirmed their excellent antibacterial activity. Chitosan/Collagen-coated Polycaprolactone/Nylon 6 mats demonstrate considerable potential as implant materials for pelvic reestablishment in pelvic organ prolapse patients.http://www.sciencedirect.com/science/article/pii/S0264127521002690Pelvic organ prolapseNylonCollagenLBL-structured matsBiocompatibility |
| spellingShingle | Fangfang Dai Jia Yu Mengqin Yuan Zhimin Deng Yanqing Wang Yaqi Fan Hongbing Deng Yanxiang Cheng Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous mats Materials & Design Pelvic organ prolapse Nylon Collagen LBL-structured mats Biocompatibility |
| title | Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous mats |
| title_full | Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous mats |
| title_fullStr | Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous mats |
| title_full_unstemmed | Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous mats |
| title_short | Enhanced cellular compatibility of chitosan/collagen multilayers LBL modified nanofibrous mats |
| title_sort | enhanced cellular compatibility of chitosan collagen multilayers lbl modified nanofibrous mats |
| topic | Pelvic organ prolapse Nylon Collagen LBL-structured mats Biocompatibility |
| url | http://www.sciencedirect.com/science/article/pii/S0264127521002690 |
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