Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology
Abstract Background Over the years, excessive use of chemical pesticides to control plant pathogens has caused environmental problems. Therefore, biological solutions such as the use of microorganisms with antimicrobial capacity become indispensable. To inhibit the growth of plant pathogens, biologi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-05-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-023-00519-4 |
| _version_ | 1797822774729768960 |
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| author | Pelias Rafanomezantsoa Samia Gharbi Noureddine Karkachi Mebrouk Kihal |
| author_facet | Pelias Rafanomezantsoa Samia Gharbi Noureddine Karkachi Mebrouk Kihal |
| author_sort | Pelias Rafanomezantsoa |
| collection | DOAJ |
| description | Abstract Background Over the years, excessive use of chemical pesticides to control plant pathogens has caused environmental problems. Therefore, biological solutions such as the use of microorganisms with antimicrobial capacity become indispensable. To inhibit the growth of plant pathogens, biological control agents use different mechanisms, including the production of hydrolytic enzymes. In this study, the production of amylase, an enzyme important for the prevention and control of plant diseases, by a biological control agent Bacillus halotolerans RFP74 was optimized using response surface methodology. Results Bacillus halotolerans RFP74 inhibited the growth of various phytopathogens including Alternaria and Bipolaris with an inhibition rate of more than 60%. In addition, it also demonstrated an essential production of amylase. Based on previous studies of amylase production in Bacillus, three parameters were considered significant: initial pH of the medium, incubation time, and temperature. Using the central composite design with Design Expert software, the optimized amylase production for B. halotolerans RFP74 is at a temperature of 37 °C, incubation time 51 h and pH 6. Conclusion The biological control agent B. halotolerans RFP74 inhibited the growth of Alternaria and Bipolaris, demonstrating its broad spectrum of activity. Knowledge of the optimal condition required for the production of hydrolytic enzymes such as amylase provides information on the most effective application of this biological control agent. |
| first_indexed | 2024-03-13T10:14:08Z |
| format | Article |
| id | doaj.art-f54a8eee73114b18bc5ac6f50fb8d233 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-03-13T10:14:08Z |
| publishDate | 2023-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-f54a8eee73114b18bc5ac6f50fb8d2332023-05-21T11:23:21ZengSpringerOpenJournal of Genetic Engineering and Biotechnology2090-59202023-05-0121111010.1186/s43141-023-00519-4Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodologyPelias Rafanomezantsoa0Samia Gharbi1Noureddine Karkachi2Mebrouk Kihal3Department of Biological Science, Applied Microbiology LaboratoryDepartment of Biotechnology, University of Science and Technology of Oran Mohamed BoudiafDepartment of Biological Science, Applied Microbiology LaboratoryDepartment of Biological Science, Applied Microbiology LaboratoryAbstract Background Over the years, excessive use of chemical pesticides to control plant pathogens has caused environmental problems. Therefore, biological solutions such as the use of microorganisms with antimicrobial capacity become indispensable. To inhibit the growth of plant pathogens, biological control agents use different mechanisms, including the production of hydrolytic enzymes. In this study, the production of amylase, an enzyme important for the prevention and control of plant diseases, by a biological control agent Bacillus halotolerans RFP74 was optimized using response surface methodology. Results Bacillus halotolerans RFP74 inhibited the growth of various phytopathogens including Alternaria and Bipolaris with an inhibition rate of more than 60%. In addition, it also demonstrated an essential production of amylase. Based on previous studies of amylase production in Bacillus, three parameters were considered significant: initial pH of the medium, incubation time, and temperature. Using the central composite design with Design Expert software, the optimized amylase production for B. halotolerans RFP74 is at a temperature of 37 °C, incubation time 51 h and pH 6. Conclusion The biological control agent B. halotolerans RFP74 inhibited the growth of Alternaria and Bipolaris, demonstrating its broad spectrum of activity. Knowledge of the optimal condition required for the production of hydrolytic enzymes such as amylase provides information on the most effective application of this biological control agent.https://doi.org/10.1186/s43141-023-00519-4AntifungalAmylaseBacillusBiocontrolOptimization |
| spellingShingle | Pelias Rafanomezantsoa Samia Gharbi Noureddine Karkachi Mebrouk Kihal Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology Journal of Genetic Engineering and Biotechnology Antifungal Amylase Bacillus Biocontrol Optimization |
| title | Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology |
| title_full | Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology |
| title_fullStr | Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology |
| title_full_unstemmed | Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology |
| title_short | Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology |
| title_sort | optimization of amylase production by the biological control agent bacillus halotolerans rfp74 using response surface methodology |
| topic | Antifungal Amylase Bacillus Biocontrol Optimization |
| url | https://doi.org/10.1186/s43141-023-00519-4 |
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