A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasion
<p>Abstract</p> <p>Background</p> <p>Proliferative vitreoretinopathy (PVR) is a leading cause of blindness after failed retinal reattachment surgery. PVR is characterized by the proliferation, migration and contraction of retinal pigmented epithelial cells (RPE), and th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-06-01
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| Series: | BMC Ophthalmology |
| Online Access: | http://www.biomedcentral.com/1471-2415/5/16 |
| _version_ | 1811311762341888000 |
|---|---|
| author | Wiedemann Peter Ehren Marianne Jin Manlin He Shikun Hoffmann Stephan Ryan Stephen J Hinton David R |
| author_facet | Wiedemann Peter Ehren Marianne Jin Manlin He Shikun Hoffmann Stephan Ryan Stephen J Hinton David R |
| author_sort | Wiedemann Peter |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Proliferative vitreoretinopathy (PVR) is a leading cause of blindness after failed retinal reattachment surgery. PVR is characterized by the proliferation, migration and contraction of retinal pigmented epithelial cells (RPE), and these cellular responses are influenced by the expression and function of integrin receptors. The effect of a cyclic integrin antagonist containing the amino acid sequence Arg-Gly-Asp-D-Phe-Val (RGDfV), specific for the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5</sub>, was investigated on basic fibroblast growth factor (bFGF), platelet derived growth factor-BB (PDGF-BB), and serum induced human RPE proliferation, migration, invasion and attachment to the extracellular matrix. Furthermore, the effects of bFGF and PDGF-BB regulated expression of integrins α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>on RPE cells was examined.</p> <p>Methods</p> <p>The effect of a cyclic integrin antagonist and a control peptide (0.01 μg/ml to 300 μg/ml) was investigated on serum or cytokine (bFGF or PDGF-BB pretreatment) induced human fetal RPE cell proliferation by H<sup>3</sup>-thymidine uptake. The effect of the cyclic integrin antagonist on RPE cell attachment onto different extracellular matrices (laminin, collagen IV, fibronectin), RPE cell invasion stimulated by PDGF-BB or serum, and migration stimulated by PDGF-BB, vascular endothelial growth factor (VEGF) or serum was explored. PDGF-BB and bFGF modulation of the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>was evaluated by flow cytometry.</p> <p>Results</p> <p>The integrin antagonist did not inhibit DNA synthesis stimulated by serum, bFGF, or PDGF-BB treatment. RPE attachment onto fibronectin was inhibited in a concentration range of 1–10 μg/ml (p < 0.05). Attachment of the RPE cells onto collagen IV and laminin was inhibited in a range of 3–10 μg/ml (p < 0.05). Serum and PDGF-BB stimulated migration was inhibited by the cyclic integrin antagonist in a concentration range of 1–10 μg/ml (p < 0.05). Furthermore, the cyclic integrin antagonist inhibited PDGF-BB stimulated RPE cell invasion through fibronectin (3μg/ml: 66% inhibition, p < 0.001). In each of these experiments, the control peptides had no significant effects. PDGF-BB and bFGF pretreatment of RPE cells increased the expression of integrin receptors α<sub>v</sub>β<sub>3 </sub>(bFGF: 1.9 fold, PDGF-BB: 2.3 fold) and α<sub>v</sub>β<sub>5 </sub>(bFGF: 2.9 fold, PDGF-BB: 1.5 fold).</p> <p>Conclusion</p> <p>A selective inhibition of the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>through a cyclic integrin antagonist is able to inhibit RPE cell attachment, migration and invasion. Since these steps are of importance for the progression of PVR, a cyclic integrin antagonist should be further evaluated for the treatment of this disease.</p> |
| first_indexed | 2024-04-13T10:24:36Z |
| format | Article |
| id | doaj.art-f54b08f399a94c069944bc414cc3733e |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-04-13T10:24:36Z |
| publishDate | 2005-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-f54b08f399a94c069944bc414cc3733e2022-12-22T02:50:23ZengBMCBMC Ophthalmology1471-24152005-06-01511610.1186/1471-2415-5-16A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasionWiedemann PeterEhren MarianneJin ManlinHe ShikunHoffmann StephanRyan Stephen JHinton David R<p>Abstract</p> <p>Background</p> <p>Proliferative vitreoretinopathy (PVR) is a leading cause of blindness after failed retinal reattachment surgery. PVR is characterized by the proliferation, migration and contraction of retinal pigmented epithelial cells (RPE), and these cellular responses are influenced by the expression and function of integrin receptors. The effect of a cyclic integrin antagonist containing the amino acid sequence Arg-Gly-Asp-D-Phe-Val (RGDfV), specific for the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5</sub>, was investigated on basic fibroblast growth factor (bFGF), platelet derived growth factor-BB (PDGF-BB), and serum induced human RPE proliferation, migration, invasion and attachment to the extracellular matrix. Furthermore, the effects of bFGF and PDGF-BB regulated expression of integrins α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>on RPE cells was examined.</p> <p>Methods</p> <p>The effect of a cyclic integrin antagonist and a control peptide (0.01 μg/ml to 300 μg/ml) was investigated on serum or cytokine (bFGF or PDGF-BB pretreatment) induced human fetal RPE cell proliferation by H<sup>3</sup>-thymidine uptake. The effect of the cyclic integrin antagonist on RPE cell attachment onto different extracellular matrices (laminin, collagen IV, fibronectin), RPE cell invasion stimulated by PDGF-BB or serum, and migration stimulated by PDGF-BB, vascular endothelial growth factor (VEGF) or serum was explored. PDGF-BB and bFGF modulation of the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>was evaluated by flow cytometry.</p> <p>Results</p> <p>The integrin antagonist did not inhibit DNA synthesis stimulated by serum, bFGF, or PDGF-BB treatment. RPE attachment onto fibronectin was inhibited in a concentration range of 1–10 μg/ml (p < 0.05). Attachment of the RPE cells onto collagen IV and laminin was inhibited in a range of 3–10 μg/ml (p < 0.05). Serum and PDGF-BB stimulated migration was inhibited by the cyclic integrin antagonist in a concentration range of 1–10 μg/ml (p < 0.05). Furthermore, the cyclic integrin antagonist inhibited PDGF-BB stimulated RPE cell invasion through fibronectin (3μg/ml: 66% inhibition, p < 0.001). In each of these experiments, the control peptides had no significant effects. PDGF-BB and bFGF pretreatment of RPE cells increased the expression of integrin receptors α<sub>v</sub>β<sub>3 </sub>(bFGF: 1.9 fold, PDGF-BB: 2.3 fold) and α<sub>v</sub>β<sub>5 </sub>(bFGF: 2.9 fold, PDGF-BB: 1.5 fold).</p> <p>Conclusion</p> <p>A selective inhibition of the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>through a cyclic integrin antagonist is able to inhibit RPE cell attachment, migration and invasion. Since these steps are of importance for the progression of PVR, a cyclic integrin antagonist should be further evaluated for the treatment of this disease.</p>http://www.biomedcentral.com/1471-2415/5/16 |
| spellingShingle | Wiedemann Peter Ehren Marianne Jin Manlin He Shikun Hoffmann Stephan Ryan Stephen J Hinton David R A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasion BMC Ophthalmology |
| title | A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasion |
| title_full | A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasion |
| title_fullStr | A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasion |
| title_full_unstemmed | A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasion |
| title_short | A selective cyclic integrin antagonist blocks the integrin receptors α<sub>v</sub>β<sub>3 </sub>and α<sub>v</sub>β<sub>5 </sub>and inhibits retinal pigment epithelium cell attachment, migration and invasion |
| title_sort | selective cyclic integrin antagonist blocks the integrin receptors α sub v sub β sub 3 sub and α sub v sub β sub 5 sub and inhibits retinal pigment epithelium cell attachment migration and invasion |
| url | http://www.biomedcentral.com/1471-2415/5/16 |
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