Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i>
The aim of this study was to use diffuse coplanar surface barrier discharge (DCSBD) non-thermal plasma for the disinfection of pine seed surfaces infected with <i>Fusarium oxysporum</i> spores. Artificially infected seeds of Scots pine (<i>Pinus sylvestris</i> L.) were treate...
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MDPI AG
2020-08-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/11/8/837 |
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| author | Magdalena Świecimska Mirela Tulik Božena Šerá Patrycja Golińska Juliána Tomeková Veronika Medvecká Helena Bujdáková Tomasz Oszako Anna Zahoranová Michal Šerý |
| author_facet | Magdalena Świecimska Mirela Tulik Božena Šerá Patrycja Golińska Juliána Tomeková Veronika Medvecká Helena Bujdáková Tomasz Oszako Anna Zahoranová Michal Šerý |
| author_sort | Magdalena Świecimska |
| collection | DOAJ |
| description | The aim of this study was to use diffuse coplanar surface barrier discharge (DCSBD) non-thermal plasma for the disinfection of pine seed surfaces infected with <i>Fusarium oxysporum</i> spores. Artificially infected seeds of Scots pine (<i>Pinus sylvestris</i> L.) were treated with plasma for the following exposure times: 1 s, 3 s, 5 s, 10 s, 15 s, 20 s, 30 s, and 60 s, and subsequently germinated on agar medium in Petri dishes at room temperature for the estimation of seed germination and disinfection effect of plasma treatment. Results of the treated samples were compared to the control samples, which were prepared as follows: seeds uninfected and non-treated with plasma (first control); seeds infected with <i>F. oxysporum</i> and non-treated with plasma (second control); and seeds infected with <i>F. oxysporum</i>, non-treated with plasma, but sterilized with 30% perhydrol (third control). Obtained results indicate that 3 s plasma treatment was an optimal time to inhibit <i>F. oxysporum</i> growth, and at the same time, increase the seed germination. In addition, our results are the first to show the practical application of non-thermal plasma in disinfecting infected Scots pine seeds and improving their germination. According to the results of this study, non-thermal plasma can serve as a seed surface disinfectant in the regeneration of different pine species. |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T18:02:42Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-f54bb3dae0044817b59fbbdd8774fbe72023-11-20T08:45:22ZengMDPI AGForests1999-49072020-08-0111883710.3390/f11080837Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i>Magdalena Świecimska0Mirela Tulik1Božena Šerá2Patrycja Golińska3Juliána Tomeková4Veronika Medvecká5Helena Bujdáková6Tomasz Oszako7Anna Zahoranová8Michal Šerý9Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87-100 Torun, PolandDepartment of Forest Botany, Warsaw University of Life Sciences (SGGW-WULS), Nowoursynowska 159, 02-776 Warsaw, PolandFaculty of Natural Sciences, Department of Landscape Ecology, Comenius University in Bratislava, Ilkovičova 6, 842 15 Bratislava, Slovak RepublicDepartment of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87-100 Torun, PolandFaculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Comenius University in Bratislava, Mlynská dolina, 842 48 Bratislava, Slovak RepublicFaculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Comenius University in Bratislava, Mlynská dolina, 842 48 Bratislava, Slovak RepublicFaculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovičova 6, 842 15 Bratislava, Slovak RepublicInstitute of Forest Research, Forest Protection Department, Braci Leśnej 3, 05-090 Sękocin Stary, PolandFaculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Comenius University in Bratislava, Mlynská dolina, 842 48 Bratislava, Slovak RepublicFaculty of Education, Department of Physics, University of South Bohemia, Jeronýmova 10, 371 15 České Budějovice, Czech RepublicThe aim of this study was to use diffuse coplanar surface barrier discharge (DCSBD) non-thermal plasma for the disinfection of pine seed surfaces infected with <i>Fusarium oxysporum</i> spores. Artificially infected seeds of Scots pine (<i>Pinus sylvestris</i> L.) were treated with plasma for the following exposure times: 1 s, 3 s, 5 s, 10 s, 15 s, 20 s, 30 s, and 60 s, and subsequently germinated on agar medium in Petri dishes at room temperature for the estimation of seed germination and disinfection effect of plasma treatment. Results of the treated samples were compared to the control samples, which were prepared as follows: seeds uninfected and non-treated with plasma (first control); seeds infected with <i>F. oxysporum</i> and non-treated with plasma (second control); and seeds infected with <i>F. oxysporum</i>, non-treated with plasma, but sterilized with 30% perhydrol (third control). Obtained results indicate that 3 s plasma treatment was an optimal time to inhibit <i>F. oxysporum</i> growth, and at the same time, increase the seed germination. In addition, our results are the first to show the practical application of non-thermal plasma in disinfecting infected Scots pine seeds and improving their germination. According to the results of this study, non-thermal plasma can serve as a seed surface disinfectant in the regeneration of different pine species.https://www.mdpi.com/1999-4907/11/8/837fungal diseaseplant pathogenseed germinationpinenon-thermal plasma treatment |
| spellingShingle | Magdalena Świecimska Mirela Tulik Božena Šerá Patrycja Golińska Juliána Tomeková Veronika Medvecká Helena Bujdáková Tomasz Oszako Anna Zahoranová Michal Šerý Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i> Forests fungal disease plant pathogen seed germination pine non-thermal plasma treatment |
| title | Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i> |
| title_full | Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i> |
| title_fullStr | Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i> |
| title_full_unstemmed | Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i> |
| title_short | Non-Thermal Plasma Can Be Used in Disinfection of Scots Pine (<i>Pinus sylvestris</i> L.) Seeds Infected with <i>Fusarium oxysporum</i> |
| title_sort | non thermal plasma can be used in disinfection of scots pine i pinus sylvestris i l seeds infected with i fusarium oxysporum i |
| topic | fungal disease plant pathogen seed germination pine non-thermal plasma treatment |
| url | https://www.mdpi.com/1999-4907/11/8/837 |
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