Electrochemical Immunosensor for in Situ Detection of Brassinolide
ObjectiveBrassinolide is an important endogenous plant hormone. In this work, an electrochemical immunosensor for in situ detection of brassinolide was constructed using screen-printed electrode (SPE).MethodsAu nanoparticles (AuNPs) were firstly electrodeposited on the surface of SPE electrode by el...
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| Format: | Article |
| Language: | English |
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Editorial Office of Smart Agriculture
2024-01-01
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| Series: | 智慧农业 |
| Subjects: | |
| Online Access: | http://www.smartag.net.cn/CN/10.12133/j.smartag.SA202311001 |
| _version_ | 1797216135597260800 |
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| author | WEI Qian GAO Yuanyuan LI Aixue |
| author_facet | WEI Qian GAO Yuanyuan LI Aixue |
| author_sort | WEI Qian |
| collection | DOAJ |
| description | ObjectiveBrassinolide is an important endogenous plant hormone. In this work, an electrochemical immunosensor for in situ detection of brassinolide was constructed using screen-printed electrode (SPE).MethodsAu nanoparticles (AuNPs) were firstly electrodeposited on the surface of SPE electrode by electrochemical workstation, and then CuCl2 nanowires (CuCl2 NWs) were added to the electrode, which can not only increase the conductivity of the electrode, but also Cu2+ can be used as a REDOX probe for the sensor. Finally, Mxene and polydopamine nanocomposite (Mxene@PDA) were selected as the modification materials for SPE electrodes because Mxene has the advantages of large surface area and good electrical conductivity, which can further amplify Cu2+ signals. However, Mxene is easily oxidized and unstable in air. Polydopamine (PDA) contains a large number of catechol and amino groups, which are coated on the surface of Mxene after self-polymerization by dopamine, cutting off the path of oxygen penetration, making Mxene difficult to be oxidized. Mxene@PDA can also be used as a coupling agent to fix more antibodies on the electrode surface, improving the overall biocompatibility, and improve the overall biocompatibility.Results and DiscussionsThe sensor has a wide linear detection range: 0.1 pg/mL to 1 mg/mL, and the detection limit was 0.015 pg/ml (S/N=3). In addition, the content of endogenous brassinolide in wheat was detected by SPE electrodes in vitro and the recovery rate was 98.13% to 104.74%.While verifying the accuracy of the sensor, it also demonstrated its superior stability and sensitivity. Besides, the sensor also showed excellent application potential in the in situ detection of brassinosteroids from wheat leaves. Compared with other brassinolide detection methods, the immunosensor developed in this study has better analytical performance.ConclusionsAn electrochemical immunosensor for in situ detection of brassinolide was developed for the first time, providing a good electrochemical platform for in situ determination of brassinolide in plant leaves, which has great application potential in precision agriculture. |
| first_indexed | 2024-04-24T11:41:09Z |
| format | Article |
| id | doaj.art-f54cbd3ecdd14da480a9d97478b85870 |
| institution | Directory Open Access Journal |
| issn | 2096-8094 |
| language | English |
| last_indexed | 2024-04-24T11:41:09Z |
| publishDate | 2024-01-01 |
| publisher | Editorial Office of Smart Agriculture |
| record_format | Article |
| series | 智慧农业 |
| spelling | doaj.art-f54cbd3ecdd14da480a9d97478b858702024-04-10T02:14:34ZengEditorial Office of Smart Agriculture智慧农业2096-80942024-01-0161768810.12133/j.smartag.SA202311001SA202311001Electrochemical Immunosensor for in Situ Detection of BrassinolideWEI Qian0GAO Yuanyuan1LI Aixue2College of Agricultural Engineering, Jiangsu University, Zhenjiang 212000, ChinaCollege of Agricultural Engineering, Jiangsu University, Zhenjiang 212000, ChinaCollege of Agricultural Engineering, Jiangsu University, Zhenjiang 212000, ChinaObjectiveBrassinolide is an important endogenous plant hormone. In this work, an electrochemical immunosensor for in situ detection of brassinolide was constructed using screen-printed electrode (SPE).MethodsAu nanoparticles (AuNPs) were firstly electrodeposited on the surface of SPE electrode by electrochemical workstation, and then CuCl2 nanowires (CuCl2 NWs) were added to the electrode, which can not only increase the conductivity of the electrode, but also Cu2+ can be used as a REDOX probe for the sensor. Finally, Mxene and polydopamine nanocomposite (Mxene@PDA) were selected as the modification materials for SPE electrodes because Mxene has the advantages of large surface area and good electrical conductivity, which can further amplify Cu2+ signals. However, Mxene is easily oxidized and unstable in air. Polydopamine (PDA) contains a large number of catechol and amino groups, which are coated on the surface of Mxene after self-polymerization by dopamine, cutting off the path of oxygen penetration, making Mxene difficult to be oxidized. Mxene@PDA can also be used as a coupling agent to fix more antibodies on the electrode surface, improving the overall biocompatibility, and improve the overall biocompatibility.Results and DiscussionsThe sensor has a wide linear detection range: 0.1 pg/mL to 1 mg/mL, and the detection limit was 0.015 pg/ml (S/N=3). In addition, the content of endogenous brassinolide in wheat was detected by SPE electrodes in vitro and the recovery rate was 98.13% to 104.74%.While verifying the accuracy of the sensor, it also demonstrated its superior stability and sensitivity. Besides, the sensor also showed excellent application potential in the in situ detection of brassinosteroids from wheat leaves. Compared with other brassinolide detection methods, the immunosensor developed in this study has better analytical performance.ConclusionsAn electrochemical immunosensor for in situ detection of brassinolide was developed for the first time, providing a good electrochemical platform for in situ determination of brassinolide in plant leaves, which has great application potential in precision agriculture.http://www.smartag.net.cn/CN/10.12133/j.smartag.SA202311001brassinolidein situ detectionelectrochemicalaunpsimmunosensor |
| spellingShingle | WEI Qian GAO Yuanyuan LI Aixue Electrochemical Immunosensor for in Situ Detection of Brassinolide 智慧农业 brassinolide in situ detection electrochemical aunps immunosensor |
| title | Electrochemical Immunosensor for in Situ Detection of Brassinolide |
| title_full | Electrochemical Immunosensor for in Situ Detection of Brassinolide |
| title_fullStr | Electrochemical Immunosensor for in Situ Detection of Brassinolide |
| title_full_unstemmed | Electrochemical Immunosensor for in Situ Detection of Brassinolide |
| title_short | Electrochemical Immunosensor for in Situ Detection of Brassinolide |
| title_sort | electrochemical immunosensor for in situ detection of brassinolide |
| topic | brassinolide in situ detection electrochemical aunps immunosensor |
| url | http://www.smartag.net.cn/CN/10.12133/j.smartag.SA202311001 |
| work_keys_str_mv | AT weiqian electrochemicalimmunosensorforinsitudetectionofbrassinolide AT gaoyuanyuan electrochemicalimmunosensorforinsitudetectionofbrassinolide AT liaixue electrochemicalimmunosensorforinsitudetectionofbrassinolide |