| Summary: | In this study, the mitochondrial genome of Mileewa margheritae (Hemiptera: Cicadellidae: Mileewinae) was sequenced and annotated. The mitogenome of M. margheritae is 15376 bp in length, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The A + T content in the mitogenome was 79%. Most of PCGs started with ATN and stopped with TAA while ATP8 and ND5 started with TTG, COX2, ND1 stopped with incomplete T, Cytb stopped with TAG. We further constructed a Bayesian phylogenetic tree among M. margheritae, M. albovittata and other Cicadellidae species. The constructed phylogenetic tree suggests the close evolutionary relationship between M. margheritae and M. albovittata.
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