Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines
C-type natriuretic peptide (CNP) is the most conserved member of the mammalian natriuretic peptide family, and is implicated in the endocrine regulation of growth, metabolism and reproduction. CNP is expressed throughout the body, but is particularly abundant in the central nervous system and anteri...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/9/1086 |
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| author | Samantha M Mirczuk Andrew J Lessey Alice R Catterick Rebecca M Perrett Christopher J Scudder Jordan E Read Victoria J Lipscomb Stijn J Niessen Andrew J Childs Craig A McArdle Imelda M McGonnell Robert C Fowkes |
| author_facet | Samantha M Mirczuk Andrew J Lessey Alice R Catterick Rebecca M Perrett Christopher J Scudder Jordan E Read Victoria J Lipscomb Stijn J Niessen Andrew J Childs Craig A McArdle Imelda M McGonnell Robert C Fowkes |
| author_sort | Samantha M Mirczuk |
| collection | DOAJ |
| description | C-type natriuretic peptide (CNP) is the most conserved member of the mammalian natriuretic peptide family, and is implicated in the endocrine regulation of growth, metabolism and reproduction. CNP is expressed throughout the body, but is particularly abundant in the central nervous system and anterior pituitary gland. Pituitary gonadotropes are regulated by pulsatile release of gonadotropin releasing hormone (GnRH) from the hypothalamus, to control reproductive function. GnRH and CNP reciprocally regulate their respective signalling pathways in αT3-1 gonadotrope cells, but effects of pulsatile GnRH stimulation on CNP expression has not been explored. Here, we examine the sensitivity of the natriuretic peptide system in LβT2 and αT3-1 gonadotrope cell lines to continuous and pulsatile GnRH stimulation, and investigate putative CNP target genes in gonadotropes. Multiplex RT-qPCR assays confirmed that primary mouse pituitary tissue express <i>Nppc,</i> <i>Npr2</i> (encoding CNP and guanylyl cyclase B (GC-B), respectively) and <i>Furin</i> (a CNP processing enzyme), but failed to express transcripts for <i>Nppa</i> or <i>Nppb</i> (encoding ANP and BNP, respectively). Pulsatile, but not continuous, GnRH stimulation of LβT2 cells caused significant increases in <i>Nppc</i> and <i>Npr2</i> expression within 4 h, but failed to alter natriuretic peptide gene expression in αT3-1 cells. CNP enhanced expression of <i>cJun</i>, <i>Egr1</i>, <i>Nr5a1</i> and <i>Nr0b1</i>, within 8 h in LβT2 cells, but inhibited <i>Nr5a1</i> expression in αT3-1 cells. Collectively, these data show the gonadotrope natriuretic peptide system is sensitive to pulsatile GnRH signalling, and gonadotrope transcription factors are putative CNP-target genes. Such findings represent additional mechanisms by which CNP may regulate reproductive function. |
| first_indexed | 2024-03-12T19:13:38Z |
| format | Article |
| id | doaj.art-f54fc781a0414f39b5e1f910f41b64d7 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T19:13:38Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-f54fc781a0414f39b5e1f910f41b64d72023-08-02T05:42:42ZengMDPI AGCells2073-44092019-09-0189108610.3390/cells8091086cells8091086Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell LinesSamantha M Mirczuk0Andrew J Lessey1Alice R Catterick2Rebecca M Perrett3Christopher J Scudder4Jordan E Read5Victoria J Lipscomb6Stijn J Niessen7Andrew J Childs8Craig A McArdle9Imelda M McGonnell10Robert C Fowkes11Endocrine Signalling Group, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKEndocrine Signalling Group, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKEndocrine Signalling Group, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKLaboratories for Integrative Neuroscience and Endocrinology, Department of Clinical Sciences at South Bristol, University of Bristol, Whitson Street, Bristol BS13NY, UKEndocrine Signalling Group, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKEndocrine Signalling Group, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKClinical Science and Services, Royal Veterinary College, Hertfordshire AL9 7TA, UKClinical Science and Services, Royal Veterinary College, Hertfordshire AL9 7TA, UKComparative Biomedical Sciences, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKLaboratories for Integrative Neuroscience and Endocrinology, Department of Clinical Sciences at South Bristol, University of Bristol, Whitson Street, Bristol BS13NY, UKComparative Biomedical Sciences, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKEndocrine Signalling Group, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UKC-type natriuretic peptide (CNP) is the most conserved member of the mammalian natriuretic peptide family, and is implicated in the endocrine regulation of growth, metabolism and reproduction. CNP is expressed throughout the body, but is particularly abundant in the central nervous system and anterior pituitary gland. Pituitary gonadotropes are regulated by pulsatile release of gonadotropin releasing hormone (GnRH) from the hypothalamus, to control reproductive function. GnRH and CNP reciprocally regulate their respective signalling pathways in αT3-1 gonadotrope cells, but effects of pulsatile GnRH stimulation on CNP expression has not been explored. Here, we examine the sensitivity of the natriuretic peptide system in LβT2 and αT3-1 gonadotrope cell lines to continuous and pulsatile GnRH stimulation, and investigate putative CNP target genes in gonadotropes. Multiplex RT-qPCR assays confirmed that primary mouse pituitary tissue express <i>Nppc,</i> <i>Npr2</i> (encoding CNP and guanylyl cyclase B (GC-B), respectively) and <i>Furin</i> (a CNP processing enzyme), but failed to express transcripts for <i>Nppa</i> or <i>Nppb</i> (encoding ANP and BNP, respectively). Pulsatile, but not continuous, GnRH stimulation of LβT2 cells caused significant increases in <i>Nppc</i> and <i>Npr2</i> expression within 4 h, but failed to alter natriuretic peptide gene expression in αT3-1 cells. CNP enhanced expression of <i>cJun</i>, <i>Egr1</i>, <i>Nr5a1</i> and <i>Nr0b1</i>, within 8 h in LβT2 cells, but inhibited <i>Nr5a1</i> expression in αT3-1 cells. Collectively, these data show the gonadotrope natriuretic peptide system is sensitive to pulsatile GnRH signalling, and gonadotrope transcription factors are putative CNP-target genes. Such findings represent additional mechanisms by which CNP may regulate reproductive function.https://www.mdpi.com/2073-4409/8/9/1086natriuretic peptidesgonadotropin releasing hormone (GnRH) signalinggonadotropegene expressioncGMP |
| spellingShingle | Samantha M Mirczuk Andrew J Lessey Alice R Catterick Rebecca M Perrett Christopher J Scudder Jordan E Read Victoria J Lipscomb Stijn J Niessen Andrew J Childs Craig A McArdle Imelda M McGonnell Robert C Fowkes Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines Cells natriuretic peptides gonadotropin releasing hormone (GnRH) signaling gonadotrope gene expression cGMP |
| title | Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines |
| title_full | Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines |
| title_fullStr | Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines |
| title_full_unstemmed | Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines |
| title_short | Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines |
| title_sort | regulation and function of c type natriuretic peptide cnp in gonadotrope derived cell lines |
| topic | natriuretic peptides gonadotropin releasing hormone (GnRH) signaling gonadotrope gene expression cGMP |
| url | https://www.mdpi.com/2073-4409/8/9/1086 |
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