Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study
AbstractPurpose Feline soft tissue sarcoma (STS) and injection site sarcoma (fISS) are rapidly growing tumors with low metastatic potential, but locally aggressive behavior. Histotripsy is a non-invasive focused ultrasound therapy using controlled acoustic cavitation to mechanically disintegrate tis...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | International Journal of Hyperthermia |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/02656736.2023.2210272 |
| _version_ | 1797374262440361984 |
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| author | Lauren Ruger Ester Yang Sheryl Coutermarsh-Ott Elliana Vickers Jessica Gannon Marlie Nightengale Andy Hsueh Brittany Ciepluch Nikolaos Dervisis Eli Vlaisavljevich Shawna Klahn |
| author_facet | Lauren Ruger Ester Yang Sheryl Coutermarsh-Ott Elliana Vickers Jessica Gannon Marlie Nightengale Andy Hsueh Brittany Ciepluch Nikolaos Dervisis Eli Vlaisavljevich Shawna Klahn |
| author_sort | Lauren Ruger |
| collection | DOAJ |
| description | AbstractPurpose Feline soft tissue sarcoma (STS) and injection site sarcoma (fISS) are rapidly growing tumors with low metastatic potential, but locally aggressive behavior. Histotripsy is a non-invasive focused ultrasound therapy using controlled acoustic cavitation to mechanically disintegrate tissue. In this study, we investigated the in vivo safety and feasibility of histotripsy to treat fISS using a custom 1 MHz transducer.Materials and Methods Three cats with naturally-occurring STS were treated with histotripsy before surgical removal of the tumor 3 to 6 days later. Gross and histological analyses were used to characterize the ablation efficacy of the treatment, and routine immunohistochemistry and batched cytokine analysis were used to investigate the acute immunological effects of histotripsy.Results Results showed that histotripsy ablation was achievable and well-tolerated in all three cats. Precise cavitation bubble clouds were generated in all patients, and hematoxylin & eosin stained tissues revealed ablative damage in targeted regions. Immunohistochemical results identified an increase in IBA-1 positive cells in treated tissues, and no significant changes in cytokine concentrations were identified post-treatment.Conclusions Overall, the results of this study demonstrate the safety and feasibility of histotripsy to target and ablate superficial feline STS and fISS tumors and guide the clinical development of histotripsy devices for this application. |
| first_indexed | 2024-03-08T19:03:19Z |
| format | Article |
| id | doaj.art-f54ff1e12a374cfb90ceb8c6a34b6b58 |
| institution | Directory Open Access Journal |
| issn | 0265-6736 1464-5157 |
| language | English |
| last_indexed | 2024-03-08T19:03:19Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Hyperthermia |
| spelling | doaj.art-f54ff1e12a374cfb90ceb8c6a34b6b582023-12-28T04:15:43ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572023-12-0140110.1080/02656736.2023.2210272Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility studyLauren Ruger0Ester Yang1Sheryl Coutermarsh-Ott2Elliana Vickers3Jessica Gannon4Marlie Nightengale5Andy Hsueh6Brittany Ciepluch7Nikolaos Dervisis8Eli Vlaisavljevich9Shawna Klahn10Department of Biomedical Engineering and Mechanics, VA Polytechnic Institute and State University, Blacksburg, VA, USADepartment of Small Animal Clinical Sciences, Virginia-MD College of Veterinary Medicine, Blacksburg, VA, USABiomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USADepartment of Biomedical Engineering and Mechanics, VA Polytechnic Institute and State University, Blacksburg, VA, USADepartment of Biomedical Engineering and Mechanics, VA Polytechnic Institute and State University, Blacksburg, VA, USADepartment of Small Animal Clinical Sciences, Virginia-MD College of Veterinary Medicine, Blacksburg, VA, USADepartment of Small Animal Clinical Sciences, Virginia-MD College of Veterinary Medicine, Blacksburg, VA, USADepartment of Small Animal Clinical Sciences, Virginia-MD College of Veterinary Medicine, Blacksburg, VA, USADepartment of Small Animal Clinical Sciences, Virginia-MD College of Veterinary Medicine, Blacksburg, VA, USADepartment of Biomedical Engineering and Mechanics, VA Polytechnic Institute and State University, Blacksburg, VA, USADepartment of Small Animal Clinical Sciences, Virginia-MD College of Veterinary Medicine, Blacksburg, VA, USAAbstractPurpose Feline soft tissue sarcoma (STS) and injection site sarcoma (fISS) are rapidly growing tumors with low metastatic potential, but locally aggressive behavior. Histotripsy is a non-invasive focused ultrasound therapy using controlled acoustic cavitation to mechanically disintegrate tissue. In this study, we investigated the in vivo safety and feasibility of histotripsy to treat fISS using a custom 1 MHz transducer.Materials and Methods Three cats with naturally-occurring STS were treated with histotripsy before surgical removal of the tumor 3 to 6 days later. Gross and histological analyses were used to characterize the ablation efficacy of the treatment, and routine immunohistochemistry and batched cytokine analysis were used to investigate the acute immunological effects of histotripsy.Results Results showed that histotripsy ablation was achievable and well-tolerated in all three cats. Precise cavitation bubble clouds were generated in all patients, and hematoxylin & eosin stained tissues revealed ablative damage in targeted regions. Immunohistochemical results identified an increase in IBA-1 positive cells in treated tissues, and no significant changes in cytokine concentrations were identified post-treatment.Conclusions Overall, the results of this study demonstrate the safety and feasibility of histotripsy to target and ablate superficial feline STS and fISS tumors and guide the clinical development of histotripsy devices for this application.https://www.tandfonline.com/doi/10.1080/02656736.2023.2210272Soft tissue sarcomafelinehistotripsyfocused ultrasoundcancerablation |
| spellingShingle | Lauren Ruger Ester Yang Sheryl Coutermarsh-Ott Elliana Vickers Jessica Gannon Marlie Nightengale Andy Hsueh Brittany Ciepluch Nikolaos Dervisis Eli Vlaisavljevich Shawna Klahn Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study International Journal of Hyperthermia Soft tissue sarcoma feline histotripsy focused ultrasound cancer ablation |
| title | Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study |
| title_full | Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study |
| title_fullStr | Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study |
| title_full_unstemmed | Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study |
| title_short | Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study |
| title_sort | histotripsy ablation for the treatment of feline injection site sarcomas a first in cat in vivo feasibility study |
| topic | Soft tissue sarcoma feline histotripsy focused ultrasound cancer ablation |
| url | https://www.tandfonline.com/doi/10.1080/02656736.2023.2210272 |
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