INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS
Pomegranate peel contains bioactive ingredients such as flavonoids, ellagitannins, phenolics and proanthocyanidin compounds with high antioxidant activity. Pomegranate peel has antiapoptotic, antioxidant and anti-inflammatory effects due to its high punicalagin content. We aimed to determine the eff...
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| Format: | Article |
| Language: | English |
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Trakya University
2023-04-01
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| Series: | Trakya University Journal of Natural Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/2545093 |
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| author | Duygu Köse Muhammed Yayla Pinar Aksu Kılıçle Zekai Halıcı Elif Çadırcı Erdem Toktay Rüstem Anıl Uğan Tuğba Nurcan Yüksel |
| author_facet | Duygu Köse Muhammed Yayla Pinar Aksu Kılıçle Zekai Halıcı Elif Çadırcı Erdem Toktay Rüstem Anıl Uğan Tuğba Nurcan Yüksel |
| author_sort | Duygu Köse |
| collection | DOAJ |
| description | Pomegranate peel contains bioactive ingredients such as flavonoids, ellagitannins, phenolics and proanthocyanidin compounds with high antioxidant activity. Pomegranate peel has antiapoptotic, antioxidant and anti-inflammatory effects due to its high punicalagin content. We aimed to determine the effect of pomegranate peel extract (PPE) on lipopolysaccharide (LPS)-induced uveitis. Sixty rats were seperated randomly into twelve groups (n = 5). The healthy group received intraperitoneal normal saline, the uveitis group received 200 μg/kg LPS, the dexamethasone (DEX) group received 200 μg/kg LPS plus 1 mg/kg DEX, the PPE100, PPE300 and PPE500 groups received 200 μg/kg LPS plus 100, 300 and 500 mg/kg PPE, respectively. The eye tissues were collected at 3rd and 24th hour. and investigated molecularly (Relative quantification of gene expression), biochemically (Superoxide dismutase activity, Glutathione and Malondialdehyde levels) and histopathologically (staining with Harris Hematoxylin and Eosin Y). Tumor Necrosis Factor-α, vascular endothelial growth factor, and Caspase-3 levels markedly decreased in a dose-dependent manner in the uveitic rats following PPE administration. PPE administration significantly ameliorated uveitic disorders in oxidative stress factors including Glutathione, Superoxide dismutase and Malondialdehyde, with its effects raising in a dose-dependent manner. PPE eliminated histopathological changes in eye tissues due to uveitis. PPE can be a promising agent by contributing to alternative preventive treatment methods for uveitis with its anti-inflammatory, antioxidative, antiapoptotic and antiangiogenic effects. |
| first_indexed | 2024-03-11T12:01:14Z |
| format | Article |
| id | doaj.art-f550c17525264a93885c68f549ca15be |
| institution | Directory Open Access Journal |
| issn | 2528-9691 |
| language | English |
| last_indexed | 2024-03-11T12:01:14Z |
| publishDate | 2023-04-01 |
| publisher | Trakya University |
| record_format | Article |
| series | Trakya University Journal of Natural Sciences |
| spelling | doaj.art-f550c17525264a93885c68f549ca15be2023-11-08T07:27:55ZengTrakya UniversityTrakya University Journal of Natural Sciences2528-96912023-04-01241112010.23902/trkjnat.114546269INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATSDuygu Köse0Muhammed Yayla1Pinar Aksu Kılıçle2Zekai Halıcı3Elif Çadırcı4Erdem Toktay5Rüstem Anıl Uğan6Tuğba Nurcan Yüksel7KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİKAFKAS ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, TIBBİ FARMAKOLOJİ ANABİLİM DALIKAFKAS ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR.ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, TIBBİ FARMAKOLOJİ ANABİLİM DALIATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, TIBBİ FARMAKOLOJİ ANABİLİM DALIKAFKAS ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALIATATÜRK ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, ECZACILIK MESLEK BİLİMLERİ BÖLÜMÜ, FARMAKOLOJİ ANABİLİM DALITekirdağ Namık Kemal ÜniversitesiPomegranate peel contains bioactive ingredients such as flavonoids, ellagitannins, phenolics and proanthocyanidin compounds with high antioxidant activity. Pomegranate peel has antiapoptotic, antioxidant and anti-inflammatory effects due to its high punicalagin content. We aimed to determine the effect of pomegranate peel extract (PPE) on lipopolysaccharide (LPS)-induced uveitis. Sixty rats were seperated randomly into twelve groups (n = 5). The healthy group received intraperitoneal normal saline, the uveitis group received 200 μg/kg LPS, the dexamethasone (DEX) group received 200 μg/kg LPS plus 1 mg/kg DEX, the PPE100, PPE300 and PPE500 groups received 200 μg/kg LPS plus 100, 300 and 500 mg/kg PPE, respectively. The eye tissues were collected at 3rd and 24th hour. and investigated molecularly (Relative quantification of gene expression), biochemically (Superoxide dismutase activity, Glutathione and Malondialdehyde levels) and histopathologically (staining with Harris Hematoxylin and Eosin Y). Tumor Necrosis Factor-α, vascular endothelial growth factor, and Caspase-3 levels markedly decreased in a dose-dependent manner in the uveitic rats following PPE administration. PPE administration significantly ameliorated uveitic disorders in oxidative stress factors including Glutathione, Superoxide dismutase and Malondialdehyde, with its effects raising in a dose-dependent manner. PPE eliminated histopathological changes in eye tissues due to uveitis. PPE can be a promising agent by contributing to alternative preventive treatment methods for uveitis with its anti-inflammatory, antioxidative, antiapoptotic and antiangiogenic effects.https://dergipark.org.tr/en/download/article-file/2545093anti-inflammatoryantioxidantlps-induced uveitispomegranate peelpunicalagintnf-αanti-inflammatoryantioxidantlps-induced uveitispomegranate peelpunicalagintnf-α |
| spellingShingle | Duygu Köse Muhammed Yayla Pinar Aksu Kılıçle Zekai Halıcı Elif Çadırcı Erdem Toktay Rüstem Anıl Uğan Tuğba Nurcan Yüksel INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS Trakya University Journal of Natural Sciences anti-inflammatory antioxidant lps-induced uveitis pomegranate peel punicalagin tnf-α anti-inflammatory antioxidant lps-induced uveitis pomegranate peel punicalagin tnf-α |
| title | INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS |
| title_full | INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS |
| title_fullStr | INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS |
| title_full_unstemmed | INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS |
| title_short | INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS |
| title_sort | investigation of the protective effects of pomegranate punica granatum l peel extract on lipopolysaccharide induced uveitis in rats |
| topic | anti-inflammatory antioxidant lps-induced uveitis pomegranate peel punicalagin tnf-α anti-inflammatory antioxidant lps-induced uveitis pomegranate peel punicalagin tnf-α |
| url | https://dergipark.org.tr/en/download/article-file/2545093 |
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