Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System
Cell perforation is a critical step for intracellular drug delivery and real-time biosensing of intracellular signals. In recent years, the nanostraws system has been developed to achieve intracellular drug delivery with minimal invasiveness to the cells. Repeated cell perforation via nano-system co...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/7/522 |
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| author | Aihua Zhang Jiaru Fang Ji Wang Xi Xie Hui-Jiuan Chen Gen He |
| author_facet | Aihua Zhang Jiaru Fang Ji Wang Xi Xie Hui-Jiuan Chen Gen He |
| author_sort | Aihua Zhang |
| collection | DOAJ |
| description | Cell perforation is a critical step for intracellular drug delivery and real-time biosensing of intracellular signals. In recent years, the nanostraws system has been developed to achieve intracellular drug delivery with minimal invasiveness to the cells. Repeated cell perforation via nano-system could allow delivery of multiple drugs into cells for cell editing, but the biosafety is rarely explored. In this work, a nanostraw-mediated nano-electroporation system was developed, which allowed repeated perforation of the same set of cells in a minimally invasive manner, while the biosafety aspect of this system was investigated. Highly controllable fabrication of Al<sub>2</sub>O<sub>3</sub> nanostraw arrays based on a porous polyethylene terephthalate (PET) membrane was integrated with a microfluidic device to construct the nanostraw-electroporation system. The pulse conditions and intervals of nano-electroporation were systematically optimized to achieve efficient cells perforation and maintain the viability of the cells. The cells proliferation, the early apoptosis activities after nanostraw-electroporation and the changes of gene functions and gene pathways of cells after repeated nano-electroporation were comprehensively analyzed. These results revealed that the repeated nanostraw-electroporation did not induce obvious negative effects on the cells. This work demonstrates the feasibility of repeated nano-electroporation on cells and provides a promising strategy for future biomedical applications. |
| first_indexed | 2024-03-09T12:10:10Z |
| format | Article |
| id | doaj.art-f553e9fbf2c04323b6f79fa5b16fe6d5 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T12:10:10Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f553e9fbf2c04323b6f79fa5b16fe6d52023-11-30T22:53:18ZengMDPI AGBiosensors2079-63742022-07-0112752210.3390/bios12070522Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw SystemAihua Zhang0Jiaru Fang1Ji Wang2Xi Xie3Hui-Jiuan Chen4Gen He5State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information, Technology, Sun Yat-sen University, Guangzhou 510006, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information, Technology, Sun Yat-sen University, Guangzhou 510006, ChinaThe First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information, Technology, Sun Yat-sen University, Guangzhou 510006, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information, Technology, Sun Yat-sen University, Guangzhou 510006, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information, Technology, Sun Yat-sen University, Guangzhou 510006, ChinaCell perforation is a critical step for intracellular drug delivery and real-time biosensing of intracellular signals. In recent years, the nanostraws system has been developed to achieve intracellular drug delivery with minimal invasiveness to the cells. Repeated cell perforation via nano-system could allow delivery of multiple drugs into cells for cell editing, but the biosafety is rarely explored. In this work, a nanostraw-mediated nano-electroporation system was developed, which allowed repeated perforation of the same set of cells in a minimally invasive manner, while the biosafety aspect of this system was investigated. Highly controllable fabrication of Al<sub>2</sub>O<sub>3</sub> nanostraw arrays based on a porous polyethylene terephthalate (PET) membrane was integrated with a microfluidic device to construct the nanostraw-electroporation system. The pulse conditions and intervals of nano-electroporation were systematically optimized to achieve efficient cells perforation and maintain the viability of the cells. The cells proliferation, the early apoptosis activities after nanostraw-electroporation and the changes of gene functions and gene pathways of cells after repeated nano-electroporation were comprehensively analyzed. These results revealed that the repeated nanostraw-electroporation did not induce obvious negative effects on the cells. This work demonstrates the feasibility of repeated nano-electroporation on cells and provides a promising strategy for future biomedical applications.https://www.mdpi.com/2079-6374/12/7/522nanostraw devicenano-electroporationrepeated electroporationcellular safetymicrofluidic device |
| spellingShingle | Aihua Zhang Jiaru Fang Ji Wang Xi Xie Hui-Jiuan Chen Gen He Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System Biosensors nanostraw device nano-electroporation repeated electroporation cellular safety microfluidic device |
| title | Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System |
| title_full | Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System |
| title_fullStr | Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System |
| title_full_unstemmed | Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System |
| title_short | Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System |
| title_sort | interrogation on the cellular nano interface and biosafety of repeated nano electroporation by nanostraw system |
| topic | nanostraw device nano-electroporation repeated electroporation cellular safety microfluidic device |
| url | https://www.mdpi.com/2079-6374/12/7/522 |
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