Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological funct...
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MDPI AG
2020-07-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/7/772 |
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| author | Li Zhang Yao Zhang Yijie Fan Haixia Guo Huihui Guo Jianfei Wu Hongmei Wang Yunlei Zhao Xin Lian Zhongyuan Gou Yuxiao Sun Congcong Zheng Cuixia Chen Fanchang Zeng |
| author_facet | Li Zhang Yao Zhang Yijie Fan Haixia Guo Huihui Guo Jianfei Wu Hongmei Wang Yunlei Zhao Xin Lian Zhongyuan Gou Yuxiao Sun Congcong Zheng Cuixia Chen Fanchang Zeng |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the <i>GhLETM1</i> gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the <i>GhLETM1</i> gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of <i>GhLETM1</i>. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties. |
| first_indexed | 2024-03-10T18:36:05Z |
| format | Article |
| id | doaj.art-f55439ce57f64cbea8effbdafeb14dd7 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T18:36:05Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-f55439ce57f64cbea8effbdafeb14dd72023-11-20T06:17:36ZengMDPI AGGenes2073-44252020-07-0111777210.3390/genes11070772Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in CottonLi Zhang0Yao Zhang1Yijie Fan2Haixia Guo3Huihui Guo4Jianfei Wu5Hongmei Wang6Yunlei Zhao7Xin Lian8Zhongyuan Gou9Yuxiao Sun10Congcong Zheng11Cuixia Chen12Fanchang Zeng13State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaThe homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the <i>GhLETM1</i> gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the <i>GhLETM1</i> gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of <i>GhLETM1</i>. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties.https://www.mdpi.com/2073-4425/11/7/772cotton biotechnologymale sterilityshortened filamentsindehiscent antherspollen abortiongene expression regulation |
| spellingShingle | Li Zhang Yao Zhang Yijie Fan Haixia Guo Huihui Guo Jianfei Wu Hongmei Wang Yunlei Zhao Xin Lian Zhongyuan Gou Yuxiao Sun Congcong Zheng Cuixia Chen Fanchang Zeng Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton Genes cotton biotechnology male sterility shortened filaments indehiscent anthers pollen abortion gene expression regulation |
| title | Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
| title_full | Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
| title_fullStr | Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
| title_full_unstemmed | Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
| title_short | Ectopic Expressions of the <i>GhLETM1</i> Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
| title_sort | ectopic expressions of the i ghletm1 i gene reveal sensitive dose effects on precise stamen development and male fertility in cotton |
| topic | cotton biotechnology male sterility shortened filaments indehiscent anthers pollen abortion gene expression regulation |
| url | https://www.mdpi.com/2073-4425/11/7/772 |
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