Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in rats
This experiment was designed to explore the effect and mechanism of electroacupuncture (EA) for hyperlipidemia and hepatic cholesterol synthesis in rats. Liver and adipose tissues were assessed histologically, and body and liver weight, serum and liver lipid levels, expression of mTOR/ubiquitin-spec...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023082130 |
| _version_ | 1827780268969164800 |
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| author | Shu-wen Jin Yong-li Han Xiao-li Pan Hong-xing Zhang |
| author_facet | Shu-wen Jin Yong-li Han Xiao-li Pan Hong-xing Zhang |
| author_sort | Shu-wen Jin |
| collection | DOAJ |
| description | This experiment was designed to explore the effect and mechanism of electroacupuncture (EA) for hyperlipidemia and hepatic cholesterol synthesis in rats. Liver and adipose tissues were assessed histologically, and body and liver weight, serum and liver lipid levels, expression of mTOR/ubiquitin-specific peptidase 20 (USP20)/recombinant 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), and phosphorylation of mTOR and USP20 were measured. In vitro deubiquitination assays with liver cytosol were conducted. EA at Fenglong point ameliorated hyperlipidemia and hepatocyte steatosis, and decreased p-USP20, p-mTOR and HMGCR expression in the liver by reducing deubiquitination. Furthermore, EA decreased feeding-induced lipid biosynthesis in the liver. Concomitantly, EA prevented the induction of phosphorylated USP20 and mTOR, and HMGCR expression; and reduced the deubiquitination of HMGCR after re-feeding. This experiment demonstrated that EA can effectively improve hyperlipidemia and reduce hepatic cholesterol synthesis by counteracting the deubiquitination activity of HMGCR in hyperlipidemic rats. |
| first_indexed | 2024-03-11T15:02:19Z |
| format | Article |
| id | doaj.art-f556ab1a31cb4295b14587ef7cd2c44d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T15:02:19Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f556ab1a31cb4295b14587ef7cd2c44d2023-10-30T06:08:05ZengElsevierHeliyon2405-84402023-10-01910e21005Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in ratsShu-wen Jin0Yong-li Han1Xiao-li Pan2Hong-xing Zhang3College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, 430061, ChinaCollege of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, 430061, ChinaCollege of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, 430061, China; Corresponding authorCollege of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, No. 188 Tanhualin Road, Wuhan, Hubei 430061, ChinaCollege of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, 430061, China; School of Medicine, Jianghan University, Wuhan, Hubei, 430056, China; Corresponding authorCollege of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, No. 188 Tanhualin Road, Wuhan, Hubei 430061, ChinaThis experiment was designed to explore the effect and mechanism of electroacupuncture (EA) for hyperlipidemia and hepatic cholesterol synthesis in rats. Liver and adipose tissues were assessed histologically, and body and liver weight, serum and liver lipid levels, expression of mTOR/ubiquitin-specific peptidase 20 (USP20)/recombinant 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), and phosphorylation of mTOR and USP20 were measured. In vitro deubiquitination assays with liver cytosol were conducted. EA at Fenglong point ameliorated hyperlipidemia and hepatocyte steatosis, and decreased p-USP20, p-mTOR and HMGCR expression in the liver by reducing deubiquitination. Furthermore, EA decreased feeding-induced lipid biosynthesis in the liver. Concomitantly, EA prevented the induction of phosphorylated USP20 and mTOR, and HMGCR expression; and reduced the deubiquitination of HMGCR after re-feeding. This experiment demonstrated that EA can effectively improve hyperlipidemia and reduce hepatic cholesterol synthesis by counteracting the deubiquitination activity of HMGCR in hyperlipidemic rats.http://www.sciencedirect.com/science/article/pii/S2405844023082130HyperlipidemiaHepatic cholesterol synthesisElectroacupunctureDeubiquitinationRecombinant 3-hydroxy-3-Methylglutaryl coenzyme A reductase |
| spellingShingle | Shu-wen Jin Yong-li Han Xiao-li Pan Hong-xing Zhang Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in rats Heliyon Hyperlipidemia Hepatic cholesterol synthesis Electroacupuncture Deubiquitination Recombinant 3-hydroxy-3-Methylglutaryl coenzyme A reductase |
| title | Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in rats |
| title_full | Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in rats |
| title_fullStr | Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in rats |
| title_full_unstemmed | Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in rats |
| title_short | Electroacupuncture regulates hepatic cholesterol synthesis by HMGCR deubiquitination in rats |
| title_sort | electroacupuncture regulates hepatic cholesterol synthesis by hmgcr deubiquitination in rats |
| topic | Hyperlipidemia Hepatic cholesterol synthesis Electroacupuncture Deubiquitination Recombinant 3-hydroxy-3-Methylglutaryl coenzyme A reductase |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023082130 |
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