Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation
Cell sheet engineering, a scaffold-free tissue fabrication technique, has proven to be an important breakthrough technology in regenerative medicine. Over the past two decades, the field has developed rapidly in terms of investigating fabrication techniques and multipurpose applications in regenerat...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/10/2/211 |
| _version_ | 1797622317677805568 |
|---|---|
| author | Parichut Thummarati Wanida Laiwattanapaisal Rikiya Nitta Megumi Fukuda Artchaya Hassametto Masahiro Kino-oka |
| author_facet | Parichut Thummarati Wanida Laiwattanapaisal Rikiya Nitta Megumi Fukuda Artchaya Hassametto Masahiro Kino-oka |
| author_sort | Parichut Thummarati |
| collection | DOAJ |
| description | Cell sheet engineering, a scaffold-free tissue fabrication technique, has proven to be an important breakthrough technology in regenerative medicine. Over the past two decades, the field has developed rapidly in terms of investigating fabrication techniques and multipurpose applications in regenerative medicine and biological research. This review highlights the most important achievements in cell sheet engineering to date. We first discuss cell sheet harvesting systems, which have been introduced in temperature-responsive surfaces and other systems to overcome the limitations of conventional cell harvesting methods. In addition, we describe several techniques of cell sheet transfer for preclinical (in vitro and in vivo) and clinical trials. This review also covers cell sheet cryopreservation, which allows short- and long-term storage of cells. Subsequently, we discuss the cell sheet properties of angiogenic cytokines and vasculogenesis. Finally, we discuss updates to various applications, from biological research to clinical translation. We believe that the present review, which shows and compares fundamental technologies and recent advances in cell engineering, can potentially be helpful for new and experienced researchers to promote the further development of tissue engineering in different applications. |
| first_indexed | 2024-03-11T09:09:31Z |
| format | Article |
| id | doaj.art-f55fab0eb158427682ecde8df1acfcd6 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T09:09:31Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-f55fab0eb158427682ecde8df1acfcd62023-11-16T19:11:13ZengMDPI AGBioengineering2306-53542023-02-0110221110.3390/bioengineering10020211Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical TranslationParichut Thummarati0Wanida Laiwattanapaisal1Rikiya Nitta2Megumi Fukuda3Artchaya Hassametto4Masahiro Kino-oka5Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanBiosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanDepartment of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanCell sheet engineering, a scaffold-free tissue fabrication technique, has proven to be an important breakthrough technology in regenerative medicine. Over the past two decades, the field has developed rapidly in terms of investigating fabrication techniques and multipurpose applications in regenerative medicine and biological research. This review highlights the most important achievements in cell sheet engineering to date. We first discuss cell sheet harvesting systems, which have been introduced in temperature-responsive surfaces and other systems to overcome the limitations of conventional cell harvesting methods. In addition, we describe several techniques of cell sheet transfer for preclinical (in vitro and in vivo) and clinical trials. This review also covers cell sheet cryopreservation, which allows short- and long-term storage of cells. Subsequently, we discuss the cell sheet properties of angiogenic cytokines and vasculogenesis. Finally, we discuss updates to various applications, from biological research to clinical translation. We believe that the present review, which shows and compares fundamental technologies and recent advances in cell engineering, can potentially be helpful for new and experienced researchers to promote the further development of tissue engineering in different applications.https://www.mdpi.com/2306-5354/10/2/211cell sheet techniquetissue engineeringregenerative medicinetemperature-responsive polymertransplantation |
| spellingShingle | Parichut Thummarati Wanida Laiwattanapaisal Rikiya Nitta Megumi Fukuda Artchaya Hassametto Masahiro Kino-oka Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation Bioengineering cell sheet technique tissue engineering regenerative medicine temperature-responsive polymer transplantation |
| title | Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation |
| title_full | Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation |
| title_fullStr | Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation |
| title_full_unstemmed | Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation |
| title_short | Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation |
| title_sort | recent advances in cell sheet engineering from fabrication to clinical translation |
| topic | cell sheet technique tissue engineering regenerative medicine temperature-responsive polymer transplantation |
| url | https://www.mdpi.com/2306-5354/10/2/211 |
| work_keys_str_mv | AT parichutthummarati recentadvancesincellsheetengineeringfromfabricationtoclinicaltranslation AT wanidalaiwattanapaisal recentadvancesincellsheetengineeringfromfabricationtoclinicaltranslation AT rikiyanitta recentadvancesincellsheetengineeringfromfabricationtoclinicaltranslation AT megumifukuda recentadvancesincellsheetengineeringfromfabricationtoclinicaltranslation AT artchayahassametto recentadvancesincellsheetengineeringfromfabricationtoclinicaltranslation AT masahirokinooka recentadvancesincellsheetengineeringfromfabricationtoclinicaltranslation |