Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
Abstract Background Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-018-5423-1 |
| _version_ | 1828766435547021312 |
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| author | Guilherme Campos Tavares Felipe Luiz Pereira Gustavo Morais Barony Cristiana Perdigão Rezende Wanderson Marques da Silva Gustavo Henrique Martins Ferreira de Souza Thiago Verano-Braga Vasco Ariston de Carvalho Azevedo Carlos Augusto Gomes Leal Henrique César Pereira Figueiredo |
| author_facet | Guilherme Campos Tavares Felipe Luiz Pereira Gustavo Morais Barony Cristiana Perdigão Rezende Wanderson Marques da Silva Gustavo Henrique Martins Ferreira de Souza Thiago Verano-Braga Vasco Ariston de Carvalho Azevedo Carlos Augusto Gomes Leal Henrique César Pereira Figueiredo |
| author_sort | Guilherme Campos Tavares |
| collection | DOAJ |
| description | Abstract Background Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid chromatography-ultra definition mass spectrometry (nanoLC-UDMSE) approach and to compare the differential abundance of proteins identified in strains isolated from GBS-infected fishes and humans. Results A total of 1070 protein clusters were identified by nanoLC-UDMSE in 5 fish-adapted GBS strains belonging to sequence types ST-260 and ST-927 and the non-typeable (NT) lineage and 1 human GBS strain (ST-23). A total of 1065 protein clusters corresponded to the pan-proteome of fish-adapted GBS strains; 989 of these were identified in all fish-adapted GBS strains (core proteome), and 62 were shared by at least two strains (accessory proteome). Proteins involved in the stress response and in the regulation of gene expression, metabolism and virulence were detected, reflecting the adaptive ability of fish-adapted GBS strains in response to stressor factors that affect bacterial survival in the aquatic environment and bacterial survival and multiplication inside the host cell. Measurement of protein abundance among different hosts showed that 5 and 26 proteins were exclusively found in the human- and fish-adapted GBS strains, respectively; the proteins exclusively identified in fish isolates were mainly related to virulence factors. Furthermore, 215 and 269 proteins were up- and down-regulated, respectively, in the fish-adapted GBS strains in comparison to the human isolate. Conclusions Our study showed that the core proteome of fish-adapted GBS strains is conserved and demonstrated high similarity of the proteins expressed by fish-adapted strains to the proteome of the human GBS strain. This high degree of proteome conservation of different STs suggests that, a monovalent vaccine may be effective against these variants. |
| first_indexed | 2024-12-11T07:05:55Z |
| format | Article |
| id | doaj.art-f562bde391394c5b8ba7eb980ec5b43d |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T07:05:55Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-f562bde391394c5b8ba7eb980ec5b43d2022-12-22T01:16:30ZengBMCBMC Genomics1471-21642019-01-0120112210.1186/s12864-018-5423-1Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approachGuilherme Campos Tavares0Felipe Luiz Pereira1Gustavo Morais Barony2Cristiana Perdigão Rezende3Wanderson Marques da Silva4Gustavo Henrique Martins Ferreira de Souza5Thiago Verano-Braga6Vasco Ariston de Carvalho Azevedo7Carlos Augusto Gomes Leal8Henrique César Pereira Figueiredo9AQUACEN – National Reference Laboratory of Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Veterinary School, Federal University of Minas GeraisAQUACEN – National Reference Laboratory of Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Veterinary School, Federal University of Minas GeraisAQUACEN – National Reference Laboratory of Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Veterinary School, Federal University of Minas GeraisAQUACEN – National Reference Laboratory of Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Veterinary School, Federal University of Minas GeraisLaboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas GeraisWaters Technologies Brazil, MS Applications Laboratory, Waters CorporationDepartment of Physiology and Biophysics, Institute of Biological Science, Federal University of Minas GeraisLaboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas GeraisAQUACEN – National Reference Laboratory of Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Veterinary School, Federal University of Minas GeraisAQUACEN – National Reference Laboratory of Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Veterinary School, Federal University of Minas GeraisAbstract Background Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid chromatography-ultra definition mass spectrometry (nanoLC-UDMSE) approach and to compare the differential abundance of proteins identified in strains isolated from GBS-infected fishes and humans. Results A total of 1070 protein clusters were identified by nanoLC-UDMSE in 5 fish-adapted GBS strains belonging to sequence types ST-260 and ST-927 and the non-typeable (NT) lineage and 1 human GBS strain (ST-23). A total of 1065 protein clusters corresponded to the pan-proteome of fish-adapted GBS strains; 989 of these were identified in all fish-adapted GBS strains (core proteome), and 62 were shared by at least two strains (accessory proteome). Proteins involved in the stress response and in the regulation of gene expression, metabolism and virulence were detected, reflecting the adaptive ability of fish-adapted GBS strains in response to stressor factors that affect bacterial survival in the aquatic environment and bacterial survival and multiplication inside the host cell. Measurement of protein abundance among different hosts showed that 5 and 26 proteins were exclusively found in the human- and fish-adapted GBS strains, respectively; the proteins exclusively identified in fish isolates were mainly related to virulence factors. Furthermore, 215 and 269 proteins were up- and down-regulated, respectively, in the fish-adapted GBS strains in comparison to the human isolate. Conclusions Our study showed that the core proteome of fish-adapted GBS strains is conserved and demonstrated high similarity of the proteins expressed by fish-adapted strains to the proteome of the human GBS strain. This high degree of proteome conservation of different STs suggests that, a monovalent vaccine may be effective against these variants.http://link.springer.com/article/10.1186/s12864-018-5423-1Comparative proteomicsStreptococcus agalactiaeGBSGenotypesFishHuman |
| spellingShingle | Guilherme Campos Tavares Felipe Luiz Pereira Gustavo Morais Barony Cristiana Perdigão Rezende Wanderson Marques da Silva Gustavo Henrique Martins Ferreira de Souza Thiago Verano-Braga Vasco Ariston de Carvalho Azevedo Carlos Augusto Gomes Leal Henrique César Pereira Figueiredo Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach BMC Genomics Comparative proteomics Streptococcus agalactiae GBS Genotypes Fish Human |
| title | Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach |
| title_full | Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach |
| title_fullStr | Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach |
| title_full_unstemmed | Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach |
| title_short | Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach |
| title_sort | delineation of the pan proteome of fish pathogenic streptococcus agalactiae strains using a label free shotgun approach |
| topic | Comparative proteomics Streptococcus agalactiae GBS Genotypes Fish Human |
| url | http://link.springer.com/article/10.1186/s12864-018-5423-1 |
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