Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration
Abstract Background The immobilized technology for microbial or cells has the advantages of high microbial activity, high microbial density per unit space, good tolerance, strong shock, load resistance, high processing efficiency, and high reuse rate. It is now widely used in environmental remediati...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-022-01995-y |
| _version_ | 1797977232946233344 |
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| author | Zhi-Hao Zhong Yu-Qing Zhang |
| author_facet | Zhi-Hao Zhong Yu-Qing Zhang |
| author_sort | Zhi-Hao Zhong |
| collection | DOAJ |
| description | Abstract Background The immobilized technology for microbial or cells has the advantages of high microbial activity, high microbial density per unit space, good tolerance, strong shock, load resistance, high processing efficiency, and high reuse rate. It is now widely used in environmental remediation, water quality treatment, biodegradation, food industry, chemical analysis, energy development, medicine and pharmaceuticals, and other fields. Results A novel Escherichia coli cell-immobilizing polyvinyl alcohol hydrogel membrane (ECI-PVAHM) was prepared by unidirectional nanopore dehydration (UND) from a 10% polyvinyl alcohol (PVA) aqueous solution containing enhanced green fluorescent protein-labeled E. coli. This bacteria-loaded film has high water stability, flexibility, transparency, and mechanical robustness. Its tensile strength, elongation rate, and swelling rate are in the ranges 0.66–0.90 MPa, 300–390%, and 330–800%, respectively. The effective bacterial load of ECI-PVAHM is 2.375 × 109–1010 CFU/g (dry weight), which does not affect the original crystal structure of the PVAHM. This biofilm has a porous network structure with pore sizes between 0.2 and 1.0 μm, and these cells are embedded in the PVAHM network. When the immobilized cells were continuously cultured for 20 days, and the medium was renewed twice daily, their relative proliferation efficiency after 40 cycles could still be maintained at ~ 91%. Conclusion The above results show that the cell division, proliferation ability, and metabolic activity of immobilized E. coli were not affected by the physical barrier of the porous network structure of the hydrogel. This UND-based ECI-PVAHM has potential applications in molecular biology, biopharmaceutical expression and production, bioreactors, and fuel cells. |
| first_indexed | 2024-04-11T05:03:41Z |
| format | Article |
| id | doaj.art-f563e8ec51e64551b64325bd68e5e832 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-11T05:03:41Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-f563e8ec51e64551b64325bd68e5e8322022-12-25T12:34:49ZengBMCMicrobial Cell Factories1475-28592022-12-0121111310.1186/s12934-022-01995-yGreen immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydrationZhi-Hao Zhong0Yu-Qing Zhang1School of Biology and Basic Medical Sciences, Medical College, Soochow UniversitySchool of Biology and Basic Medical Sciences, Medical College, Soochow UniversityAbstract Background The immobilized technology for microbial or cells has the advantages of high microbial activity, high microbial density per unit space, good tolerance, strong shock, load resistance, high processing efficiency, and high reuse rate. It is now widely used in environmental remediation, water quality treatment, biodegradation, food industry, chemical analysis, energy development, medicine and pharmaceuticals, and other fields. Results A novel Escherichia coli cell-immobilizing polyvinyl alcohol hydrogel membrane (ECI-PVAHM) was prepared by unidirectional nanopore dehydration (UND) from a 10% polyvinyl alcohol (PVA) aqueous solution containing enhanced green fluorescent protein-labeled E. coli. This bacteria-loaded film has high water stability, flexibility, transparency, and mechanical robustness. Its tensile strength, elongation rate, and swelling rate are in the ranges 0.66–0.90 MPa, 300–390%, and 330–800%, respectively. The effective bacterial load of ECI-PVAHM is 2.375 × 109–1010 CFU/g (dry weight), which does not affect the original crystal structure of the PVAHM. This biofilm has a porous network structure with pore sizes between 0.2 and 1.0 μm, and these cells are embedded in the PVAHM network. When the immobilized cells were continuously cultured for 20 days, and the medium was renewed twice daily, their relative proliferation efficiency after 40 cycles could still be maintained at ~ 91%. Conclusion The above results show that the cell division, proliferation ability, and metabolic activity of immobilized E. coli were not affected by the physical barrier of the porous network structure of the hydrogel. This UND-based ECI-PVAHM has potential applications in molecular biology, biopharmaceutical expression and production, bioreactors, and fuel cells.https://doi.org/10.1186/s12934-022-01995-yEscherichia coliImmobilizationPolyvinyl alcoholUnidirectional nanopore dehydrationHydrogelProliferation |
| spellingShingle | Zhi-Hao Zhong Yu-Qing Zhang Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration Microbial Cell Factories Escherichia coli Immobilization Polyvinyl alcohol Unidirectional nanopore dehydration Hydrogel Proliferation |
| title | Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration |
| title_full | Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration |
| title_fullStr | Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration |
| title_full_unstemmed | Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration |
| title_short | Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration |
| title_sort | green immobilization and efficient proliferation of escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration |
| topic | Escherichia coli Immobilization Polyvinyl alcohol Unidirectional nanopore dehydration Hydrogel Proliferation |
| url | https://doi.org/10.1186/s12934-022-01995-y |
| work_keys_str_mv | AT zhihaozhong greenimmobilizationandefficientproliferationofescherichiacolicellsinpolyvinylalcoholhydrogelmembranesbyunidirectionalnanoporedehydration AT yuqingzhang greenimmobilizationandefficientproliferationofescherichiacolicellsinpolyvinylalcoholhydrogelmembranesbyunidirectionalnanoporedehydration |