High resolution optical mapping of cardiac electrophysiology in pre-clinical models
Abstract Optical mapping of animal models is a widely used technique in pre-clinical cardiac research. It has several advantages over other methods, including higher spatial resolution, contactless recording and direct visualisation of action potentials and calcium transients. Optical mapping enable...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-022-01253-1 |
| _version_ | 1797350171312390144 |
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| author | Christopher O’Shea James Winter S. Nashitha Kabir Molly O’Reilly Simon P Wells Olivia Baines Laura C. Sommerfeld Joao Correia Ming Lei Paulus Kirchhof Andrew P. Holmes Larissa Fabritz Kashif Rajpoot Davor Pavlovic |
| author_facet | Christopher O’Shea James Winter S. Nashitha Kabir Molly O’Reilly Simon P Wells Olivia Baines Laura C. Sommerfeld Joao Correia Ming Lei Paulus Kirchhof Andrew P. Holmes Larissa Fabritz Kashif Rajpoot Davor Pavlovic |
| author_sort | Christopher O’Shea |
| collection | DOAJ |
| description | Abstract Optical mapping of animal models is a widely used technique in pre-clinical cardiac research. It has several advantages over other methods, including higher spatial resolution, contactless recording and direct visualisation of action potentials and calcium transients. Optical mapping enables simultaneous study of action potential and calcium transient morphology, conduction dynamics, regional heterogeneity, restitution and arrhythmogenesis. In this dataset, we have optically mapped Langendorff perfused isolated whole hearts (mouse and guinea pig) and superfused isolated atria (mouse). Raw datasets (consisting of over 400 files) can be combined with open-source software for processing and analysis. We have generated a comprehensive post-processed dataset characterising the baseline cardiac electrophysiology in these widely used pre-clinical models. This dataset also provides reference information detailing the effect of heart rate, clinically used anti-arrhythmic drugs, ischaemia-reperfusion and sympathetic nervous stimulation on cardiac electrophysiology. The effects of these interventions can be studied in a global or regional manner, enabling new insights into the prevention and initiation of arrhythmia. |
| first_indexed | 2024-03-08T12:40:54Z |
| format | Article |
| id | doaj.art-f56558f5ecfb42e4809246dad06922f8 |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-03-08T12:40:54Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj.art-f56558f5ecfb42e4809246dad06922f82024-01-21T12:10:19ZengNature PortfolioScientific Data2052-44632022-03-019111210.1038/s41597-022-01253-1High resolution optical mapping of cardiac electrophysiology in pre-clinical modelsChristopher O’Shea0James Winter1S. Nashitha Kabir2Molly O’Reilly3Simon P Wells4Olivia Baines5Laura C. Sommerfeld6Joao Correia7Ming Lei8Paulus Kirchhof9Andrew P. Holmes10Larissa Fabritz11Kashif Rajpoot12Davor Pavlovic13Institute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamDepartment of Pharmacology, University of OxfordInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamSchool of Computer Science, University of BirminghamInstitute of Cardiovascular Sciences, University of BirminghamAbstract Optical mapping of animal models is a widely used technique in pre-clinical cardiac research. It has several advantages over other methods, including higher spatial resolution, contactless recording and direct visualisation of action potentials and calcium transients. Optical mapping enables simultaneous study of action potential and calcium transient morphology, conduction dynamics, regional heterogeneity, restitution and arrhythmogenesis. In this dataset, we have optically mapped Langendorff perfused isolated whole hearts (mouse and guinea pig) and superfused isolated atria (mouse). Raw datasets (consisting of over 400 files) can be combined with open-source software for processing and analysis. We have generated a comprehensive post-processed dataset characterising the baseline cardiac electrophysiology in these widely used pre-clinical models. This dataset also provides reference information detailing the effect of heart rate, clinically used anti-arrhythmic drugs, ischaemia-reperfusion and sympathetic nervous stimulation on cardiac electrophysiology. The effects of these interventions can be studied in a global or regional manner, enabling new insights into the prevention and initiation of arrhythmia.https://doi.org/10.1038/s41597-022-01253-1 |
| spellingShingle | Christopher O’Shea James Winter S. Nashitha Kabir Molly O’Reilly Simon P Wells Olivia Baines Laura C. Sommerfeld Joao Correia Ming Lei Paulus Kirchhof Andrew P. Holmes Larissa Fabritz Kashif Rajpoot Davor Pavlovic High resolution optical mapping of cardiac electrophysiology in pre-clinical models Scientific Data |
| title | High resolution optical mapping of cardiac electrophysiology in pre-clinical models |
| title_full | High resolution optical mapping of cardiac electrophysiology in pre-clinical models |
| title_fullStr | High resolution optical mapping of cardiac electrophysiology in pre-clinical models |
| title_full_unstemmed | High resolution optical mapping of cardiac electrophysiology in pre-clinical models |
| title_short | High resolution optical mapping of cardiac electrophysiology in pre-clinical models |
| title_sort | high resolution optical mapping of cardiac electrophysiology in pre clinical models |
| url | https://doi.org/10.1038/s41597-022-01253-1 |
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