Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i>
Recently, the extensive spread of some algae along coastlines has surged into unmanageable thick decomposition layers. This study aimed to demonstrate the use of <i>Sargassum horneri</i> as a biomass resource for ethanol production through the continuous hydrolysis, enzymatic saccharific...
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MDPI AG
2023-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/12/1004 |
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| author | InYung Sunwoo Yoojin Kim Jieun Kim HyunJin Cho Gwi-Taek Jeong |
| author_facet | InYung Sunwoo Yoojin Kim Jieun Kim HyunJin Cho Gwi-Taek Jeong |
| author_sort | InYung Sunwoo |
| collection | DOAJ |
| description | Recently, the extensive spread of some algae along coastlines has surged into unmanageable thick decomposition layers. This study aimed to demonstrate the use of <i>Sargassum horneri</i> as a biomass resource for ethanol production through the continuous hydrolysis, enzymatic saccharification, and fermentation process. Sugars from <i>S. horneri</i> were obtained using a combination of thermal acid hydrolysis and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis involved a 10% (<i>w</i>/<i>v</i>) <i>S. horneri</i> slurry treated with 100 mM H<sub>2</sub>SO<sub>4</sub> at 121 °C for 60 min; enzymatic saccharification using 16 U/mL Cellic CTec2 further boosted the monosaccharide concentration to 23.53 g/L. Fermentation experiments were conducted with mannitol-adapted <i>Saccharomyces cerevisiae</i> BY4741 using <i>S. horneri</i> hydrolysate. Enhanced ethanol production was observed in the hydrolysate, particularly with mannitol-adapted <i>S. cerevisiae</i> BY4741, which yielded 10.06 g/L ethanol. Non-adapted <i>S. cerevisiae</i> produced 8.12 g/L ethanol, as it primarily utilized glucose and not mannitol. Regarding ethanol fermentation using 5 L- and 500 L-scale fermenters, the ethanol concentrations reached 10.56 g/L and 7.88 g/L with yields of 0.51 and 0.45, respectively, at 48 h. This study confirmed the economic viability of ethanol production using waste seaweed with optimized pretreatment conditions and the adaptive evolution of <i>S. cerevisiae</i> to mannitol. |
| first_indexed | 2024-03-08T20:47:21Z |
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| language | English |
| last_indexed | 2024-03-08T20:47:21Z |
| publishDate | 2023-11-01 |
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| series | Fermentation |
| spelling | doaj.art-f565caad86124952bda0aa7167da4eab2023-12-22T14:07:51ZengMDPI AGFermentation2311-56372023-11-01912100410.3390/fermentation9121004Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i>InYung Sunwoo0Yoojin Kim1Jieun Kim2HyunJin Cho3Gwi-Taek Jeong4Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of KoreaDepartment of Biotechnology, Pukyong National University, Busan 48513, Republic of KoreaMarine Biotechnology and Bioresource Research Department, Korea Institute of Ocean Science and Technology (KIOST), Busan 49111, Republic of KoreaDepartment of Biotechnology, Pukyong National University, Busan 48513, Republic of KoreaDepartment of Biotechnology, Pukyong National University, Busan 48513, Republic of KoreaRecently, the extensive spread of some algae along coastlines has surged into unmanageable thick decomposition layers. This study aimed to demonstrate the use of <i>Sargassum horneri</i> as a biomass resource for ethanol production through the continuous hydrolysis, enzymatic saccharification, and fermentation process. Sugars from <i>S. horneri</i> were obtained using a combination of thermal acid hydrolysis and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis involved a 10% (<i>w</i>/<i>v</i>) <i>S. horneri</i> slurry treated with 100 mM H<sub>2</sub>SO<sub>4</sub> at 121 °C for 60 min; enzymatic saccharification using 16 U/mL Cellic CTec2 further boosted the monosaccharide concentration to 23.53 g/L. Fermentation experiments were conducted with mannitol-adapted <i>Saccharomyces cerevisiae</i> BY4741 using <i>S. horneri</i> hydrolysate. Enhanced ethanol production was observed in the hydrolysate, particularly with mannitol-adapted <i>S. cerevisiae</i> BY4741, which yielded 10.06 g/L ethanol. Non-adapted <i>S. cerevisiae</i> produced 8.12 g/L ethanol, as it primarily utilized glucose and not mannitol. Regarding ethanol fermentation using 5 L- and 500 L-scale fermenters, the ethanol concentrations reached 10.56 g/L and 7.88 g/L with yields of 0.51 and 0.45, respectively, at 48 h. This study confirmed the economic viability of ethanol production using waste seaweed with optimized pretreatment conditions and the adaptive evolution of <i>S. cerevisiae</i> to mannitol.https://www.mdpi.com/2311-5637/9/12/1004<i>S. horneri</i>ethanol productionoptimizationadaptive evolutioncontinuous hydrolysis enzymatic saccharification and fermentation (CHEF)economical evaluation of bioenergy production |
| spellingShingle | InYung Sunwoo Yoojin Kim Jieun Kim HyunJin Cho Gwi-Taek Jeong Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i> Fermentation <i>S. horneri</i> ethanol production optimization adaptive evolution continuous hydrolysis enzymatic saccharification and fermentation (CHEF) economical evaluation of bioenergy production |
| title | Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i> |
| title_full | Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i> |
| title_fullStr | Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i> |
| title_full_unstemmed | Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i> |
| title_short | Optimization, Scale-Up, and Economic Analysis of the Ethanol Production Process Using <i>Sargassum horneri</i> |
| title_sort | optimization scale up and economic analysis of the ethanol production process using i sargassum horneri i |
| topic | <i>S. horneri</i> ethanol production optimization adaptive evolution continuous hydrolysis enzymatic saccharification and fermentation (CHEF) economical evaluation of bioenergy production |
| url | https://www.mdpi.com/2311-5637/9/12/1004 |
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